Abstract
We present a review of recent theoretical and experimental work aimed at investigating the potential use of two-phase aqueous surfactant systems for the purification of hydrophilic proteins by liquid-liquid extraction techniques. The systems studied include (1) a two-phase aqueous nonionic surfactant (n-decyl tetra(ethylene oxide), C 10 E 4) system, and (2) a two-phase aqueous zwitterionic surfactant (dioctanoyl phosphatidylcholine, C 8-lecithin) system. The theoretical formulation assumes that excluded-volume interactions between the hydrophilic proteins and the surfactant micelles present in the solution play the dominant role in determining the experimentally observed partitioning behavior, and it incorporates (i) the self-assembling character of micelles, which allows them to grow into long, cylindrical microstructures with varying temperature and/or surfactant concentration, and (ii) a broad polydisperse distribution of micellar sizes. The theoretically predicted protein partitioning is compared with experimental measurements of the partitioning of several hydrophilic proteins, including cytochrome c, soybean trypsin inhibitor, ovalbumin, bovine serum albumin, and catalase, in two-phase aqueous C 10 E 4 and C 8-lecithin systems, and is found to be in good agreement. The results of this investigation suggest that two-phase aqueous surfactant systems of the type considered in this paper are potentially useful as extractant phases for the liquid-liquid extraction of proteins and other biomaterials.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
C. Bordier, Phase separation of integral membrane proteins in Triton X-114 solution, J. Biol. Chem.,256:1604 (1981).
J.G. Pryde, Triton X-114: a detergent that has come in from the cold, TIBS, 5:160 (1986).
J.G. Pryde and J.H. Phillips, Fractionation of membrane proteins by temperature-induced phase separation in Triton X-I I4, Biochem. J.,233:525 (1986).
C. Holm, G. Fredrikson, and P. Belfrage, Demonstration of the amphiphilic character of hormone-sensitive lipase by temperature-induced phase separation in Triton X-114 and charge-shift electrophoresis, J. Biol. Chem., 261:15659 (1986).
R.A. Ramelmeier, G.C. Terstappen, and M.-R. Kula, The partitioning of cholesterol oxidase in Triton X-114-based aqueous two-phase systems, Bioseparation, 2:315 (1991).
T. Saitoh and W.L. Hinze, Concentration of hydrophobic organic compounds and extraction of protein using alkylammoniosulfate zwitterionic surfactant mediated phase separations (cloud point extractions), Anal. Chem., 63:2520 (1991).
T. Saitoh and W.L. Hinze, Use of surfactant-mediated phase separation (cloud point extraction) with affinity ligands for the extraction of hydrophilic proteins, Preprint (1992).
Y.J. Nikas, C.-L. Liu, T. Srivastava, N.L. Abbott, and D. Blankschtein, Protein partitioning in two-phase aqueous nonionic micellar solutions, Macromolecules, 25:4794 (1992).
C.-L. Liu, Y.J. Nikas, and D. Blankschtein, Partitioning of proteins using two-phase aqueous surfactant systems, AIChE J., in press (1994).
P.-Å. Albertsson, Partition of Cell Particles and Macromolecules, Wiley-Interscience, New York (1986).
H. Walter, D.E. Brooks, and D. Fisher, ed., Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology, Academic Press, Orlando (1985).
D. Fisher and I.A. Sutherland, ed., Separations Using Aqueous Phase Systems: Applications in Cell Biology and Biotechnology, Plenum Press, New York (1989).
H. Walter, G. Johansson, and D.E. Brooks, Partitioning in aqueous two-phase systems: recent results, Anal. Biochem.,197:1 (1991).
N.L. Abbott, D. Blankschtein, and T.A. Hatton, On protein partitioning in two-phase aqueous polymer systems, Bioseparation, 1:191 (1990).
N.L. Abbott, D. Blankschtein, and T.A. Hatton, Protein partitioning in two-phase aqueous polymer systems.
N.L. Abbott, D. Blankschtein, and T.A. Hatton Novel physical pictures and a scaling-thermodynamic formulation, Macromolecules, 24:4334 (1991);
N.L. Abbott, D. Blankschtein, and T.A. Hatton On the free energy of mixing globular colloids and flexible polymers, Macromolecules, 25:3917 (1992);
N.L. Abbott, D. Blankschtein, and T.A. Hatton A neutron scattering investigation of the polymer solution structure and protein-polymer interactions, Macromolecules, 25:3932 (1992);
N.L. Abbott, D. Blankschtein, and T.A. Hatton Proteins in solutions of entangled polymers, Macromolecules, 25:5192 (1992);
N.L. Abbott, D. Blankschtein, and T.A. Hatton Decoupling of the effects of protein concentration, salt type, and polymer molecular weight, Macromolecules, 26:825 (1993).
D. Blankschtein, G.M. Thurston, and G.B. Benedek, Phenomenological theory of equilibrium thermodynamic properties and phase separation of micellar solutions, J. Chem. Phys., 85:7268 (1986).
S. Puvvada and D. Blankschtein, Molecular-thermodynamic approach to predict micellization, phase behavior and phase separation of micellar solutions. I. Application to nonionic surfactants, J. Chem. Phys., 92:3710 (1990).
B. Lindman and H. Wennerström, Nonionic micelles grow with increasing temperature, J. Phys. Chem., 95:6053 (1991).
A. Helenius and K. Simons, The binding of detergents to lipophilic and hydrophilic proteins, J. Biol. Chem., 247:3656 (1972).
S. Makino, J.A. Reynolds, and C. Tanford, The binding of deoxycholate and Triton X-100 to proteins, J. Biol. Chem., 248:4926 (1973).
L.D. Landau and E.M. Lifshitz, Statistical Physics, 2nd ed., Vol. 1, Pergamon Press, Oxford (1980).
A. Naor, S. Puvvada, and D. Blankschtein, An analytical expression for the free energy of micellization, J. Phys. Chem., 96:7830 (1992).
C. Sarmoria and D. Blankschtein, Conformational characteristics of short poly(ethylene oxide) chains terminally attached to a wall and free in aqueous solution, J. Phys. Chem., 96:1978 (1992).
P.L. Dubin and J.M. Principi, Optimization of size-exclusion separation of proteins on a Superose column, J. Chromatography, 479:159 (1989).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Liu, CL., Nikas, Y.J., Blankschtein, D. (1995). Two-Phase Aqueous Surfactant Systems for the Purification of Biomaterials. In: Rogers, R.D., Eiteman, M.A. (eds) Aqueous Biphasic Separations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1953-9_4
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1953-9_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5802-2
Online ISBN: 978-1-4615-1953-9
eBook Packages: Springer Book Archive