Abstract
Partitioning in aqueous polymer two-phase systems is widely recognized as a highly efficient, versatile, and cost-effective method for separation and purification of biological materials.1-6 Aqueous two-phase systems arise in aqueous mixtures of different water-soluble polymers or a single polymer and a specific salt. When two certain polymers, e.g., dextran and polyethylene glycol are mixed in water above certain concentrations, the mixture separates into two immiscible aqueous phases. There is a clear interfacial boundary and one phase is rich in one polymer and the other phase is rich in the other. The aqueous solvent in both phases provides media suitable for biological products.
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Zaslavsky, B.Y. (1995). Use of the Aqueous Two-Phase Partition Technique for Characterization and Quality Control of Recombinant Proteins. In: Rogers, R.D., Eiteman, M.A. (eds) Aqueous Biphasic Separations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1953-9_15
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DOI: https://doi.org/10.1007/978-1-4615-1953-9_15
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