Interfacial Events in Phase Separation and Cell Partitioning in Aqueous Two-Phase Systems

  • Frank D. Raymond
  • Derek Fisher

Summary

The processes by which phase separation occurs in aqueous two-phase systems of polyethylene glycol and dextran are described in terms of the fluid dynamics of phase droplets and phase streams. Interactions of cells with the surfaces of these microphases early in phase separations determine the cell partition coefficient, which is conventionally measured when phase separation is virtually completed.

Keywords

Carbohydrate Polyethylene Glycol Electrophoresis Trypsin 

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Frank D. Raymond
    • 1
  • Derek Fisher
    • 2
  1. 1.Department of Metabolic MedicineRoyal Postgraduate Medical School Hammersmith HospitalLondonUK
  2. 2.Molecular Cell PathologyRoyal Free Hospital School of MedicineLondonUK

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