Immunostaining with Monoclonal Antibodies to Eosinophil Cationic Protein (EG1 and EG2) does not Distinguish between Resting and Activated Eosinophils in Formalin-Fixed Tissue Specimens
Activated eosinophils release highly toxic cationic proteins which are important in the defence against parasites. However, activated eosinophils may also induce tissue damage and have been reported to be associated with lesions of various allergic and inflammatory disorders. Tai et al.1 raised two monoclonal antibodies (mA) against eosinophil cationic protein (ECP); mA EG1 recognized both the stored and secreted forms of ECP, whereas mA EG2 was claimed to identify only the secretory product. mA EG2 has therefore been used in several immunohistochemical studies to identify activated eosinophils in formalin- fixed tissue specimens.1–10 The aim of our investigation was to examine whether this application of EG2 is valid.
KeywordsFormalin Acetone Trypsin Stam
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- 11.D. Mason, in: “Techniques in Immunocytochemistry”, G.R. Bullock and P. Petrusz, eds., p. 25, Academic Press (1985).Google Scholar