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Advances in Mucosal Immunology pp 283–286Cite as

Immunostaining with Monoclonal Antibodies to Eosinophil Cationic Protein (EG1 and EG2) does not Distinguish between Resting and Activated Eosinophils in Formalin-Fixed Tissue Specimens

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 371))

Abstract

Activated eosinophils release highly toxic cationic proteins which are important in the defence against parasites. However, activated eosinophils may also induce tissue damage and have been reported to be associated with lesions of various allergic and inflammatory disorders. Tai et al.1 raised two monoclonal antibodies (mA) against eosinophil cationic protein (ECP); mA EG1 recognized both the stored and secreted forms of ECP, whereas mA EG2 was claimed to identify only the secretory product. mA EG2 has therefore been used in several immunohistochemical studies to identify activated eosinophils in formalin- fixed tissue specimens.1–10 The aim of our investigation was to examine whether this application of EG2 is valid.

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References

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Authors and Affiliations

  1. LIIPAT, Institute for Pathology, The National Hospital, Rikshospitalet, University of Oslo, N-0027, Oslo, Norway

    Frode Jahnsen, Trond S. Halstensen & Per Brandtzaeg

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  1. Frode Jahnsen
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  2. Trond S. Halstensen
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  3. Per Brandtzaeg
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Editors and Affiliations

  1. University of Alabama at Birmingham, Birmingham, Alabama, USA

    Jiri Mestecky , Michael W. Russell , Susan Jackson  & Suzanne M. Michalek , ,  & 

  2. Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Helena Tlaskalová-Hogenová  & Jaroslav Šterzl  & 

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Jahnsen, F., Halstensen, T.S., Brandtzaeg, P. (1995). Immunostaining with Monoclonal Antibodies to Eosinophil Cationic Protein (EG1 and EG2) does not Distinguish between Resting and Activated Eosinophils in Formalin-Fixed Tissue Specimens. In: Mestecky, J., Russell, M.W., Jackson, S., Michalek, S.M., Tlaskalová-Hogenová, H., Šterzl, J. (eds) Advances in Mucosal Immunology. Advances in Experimental Medicine and Biology, vol 371. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1941-6_59

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  • DOI: https://doi.org/10.1007/978-1-4615-1941-6_59

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5796-4

  • Online ISBN: 978-1-4615-1941-6

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