The Effect of Medium Derived from Activated Peripheral Blood Mononuclear Cells on Two Intestinal Cell Lines

  • Ulla Kärnström
  • Ove Norén
  • Hans Sjöström
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 371)


In celiac disease both immunological reactions and gliadins are supposed to contribute to epithelial damage with villus atrophy, crypt hyperplasia and lymphocyte infiltration.1,2 A graft versus host reaction in the intestine has similar histology.3 The same kind of reaction can also be evoked experimentally in human embryonal small intestine by activation of mucosal T-lymphocytes with pokeweed mitogen (PWM).4 To further study this phenomenon, we have investigated the effect of conditioned medium from PWM stimulated peripheral blood mononuclear cells (PBMC) on the intestinal cell lines HT-29 and IEC-18.


Peripheral Blood mononucIear Cell Conditioned Medium Celiac Disease Human Peripheral Blood mononucIear Cell Pokeweed Mitogen 
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Ulla Kärnström
    • 1
  • Ove Norén
    • 1
  • Hans Sjöström
    • 1
  1. 1.Department of Biochemistry CThe Panum Institute University of CopenhagenCopenhagenDenmark

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