The Effect of Medium Derived from Activated Peripheral Blood Mononuclear Cells on Two Intestinal Cell Lines
In celiac disease both immunological reactions and gliadins are supposed to contribute to epithelial damage with villus atrophy, crypt hyperplasia and lymphocyte infiltration.1,2 A graft versus host reaction in the intestine has similar histology.3 The same kind of reaction can also be evoked experimentally in human embryonal small intestine by activation of mucosal T-lymphocytes with pokeweed mitogen (PWM).4 To further study this phenomenon, we have investigated the effect of conditioned medium from PWM stimulated peripheral blood mononuclear cells (PBMC) on the intestinal cell lines HT-29 and IEC-18.
KeywordsPeripheral Blood mononucIear Cell Conditioned Medium Celiac Disease Human Peripheral Blood mononucIear Cell Pokeweed Mitogen
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