The Effect of Medium Derived from Activated Peripheral Blood Mononuclear Cells on Two Intestinal Cell Lines

  • Ulla Kärnström
  • Ove Norén
  • Hans Sjöström
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 371)

Abstract

In celiac disease both immunological reactions and gliadins are supposed to contribute to epithelial damage with villus atrophy, crypt hyperplasia and lymphocyte infiltration.1,2 A graft versus host reaction in the intestine has similar histology.3 The same kind of reaction can also be evoked experimentally in human embryonal small intestine by activation of mucosal T-lymphocytes with pokeweed mitogen (PWM).4 To further study this phenomenon, we have investigated the effect of conditioned medium from PWM stimulated peripheral blood mononuclear cells (PBMC) on the intestinal cell lines HT-29 and IEC-18.

Keywords

Penicillin Streptomycin Thymidine Alan 

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References

  1. 1.
    A. G. F. Davidson and M. A. Bridges, Clin. Chim. Acta 163: 1 (1987).PubMedCrossRefGoogle Scholar
  2. 2.
    S. Auricchio, L. Greco, and R. Troncone, Pediat. Clin. North. Am. 35: 157 (1988).Google Scholar
  3. 3.
    M. N. Marsh and D. E. Loft, Dig. Dis. 6: 216 (1988).PubMedCrossRefGoogle Scholar
  4. 4.
    T. T. MacDonald and J. Spencer, J. Exp. Med. 167: 1341 (1988).PubMedCrossRefGoogle Scholar
  5. 5.
    R. I. Freshney, in:“Culture of Animal Cells” p. 236, Alan R. Liss, Inc., New York (1987).Google Scholar
  6. 6.
    O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, J. Biol. Chem. 193: 265 (1951).PubMedGoogle Scholar
  7. 7.
    L. E. Gerschenson and R. J. Rotello, FASEB J. 6: 2450 (1992).PubMedGoogle Scholar
  8. 8.
    J. Klein, in:“Immunology”, p. 237, Blackwell Scientific Publications, Inc., Cambridge, Massachusetts (1990).Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Ulla Kärnström
    • 1
  • Ove Norén
    • 1
  • Hans Sjöström
    • 1
  1. 1.Department of Biochemistry CThe Panum Institute University of CopenhagenCopenhagenDenmark

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