Abstract
In 1988, two groups of investigators reported the successful adoptive transfer of nonneoplastic human cells into mice with severe combined immunodeficiency (SCID).1–3 These mice originated from the CB-17 lcr strain by a spontaneous autosomal recessive mutation4 affecting the VDJ recombinase system.5 SCID mice lack functional B and T cells, but have a normal arsenal of macrophages and NK cells. The survival of human immunoreactive cells in SCID mice allows for the study of the human immune system in an animal model. Thus the human immune system is available for experimental protocols feasible up to now only for the study of animal immune systems. SCID mice engrafted with human immunologically reactive cells were called “human-mouse chimeras,” “SCID-hu mice” and, hu-SCID mice. These mice are of interest for the study of maturation and differentiation of human hematopoietic cells,6 tumor immunology,7,8 human immune responses to infectious agents, neoplasms and autoantigens,9,10 and drug effects on the human immune system. The hu-SCID mouse also seems an exciting model for the direct study of components of human mucosa-associated lymphoid tissue (MALT), their interactions, functions, and traffic in vivo.11,12
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
J. M. McCune, R. Namikawa, H. Kaneshima, L. D. Schultz, M. Lieberman, and I. L. Weissman, Science 241: 1632 (1988).
D. E. Mosier, R. J. Gulizia, S. M. Baird, and D. B. Wilson, Nature 335: 256 (1988).
P. C. Taylor, Int. J. Exp. Pathol. 73: 251 (1992).
G. C. Bosma, R. P. Custer, and M. J. Bosma, Nature 301: 527 (1983).
W. Schuler, I. J. Weiler, A. Schuler, R. A. Phillips, N. Rosenberg, T. Mak, J. F. Kearney, R. P. Perry, and M. J. Bosma, Cell 46: 963 (1986).
R. Namikawa, K. N. Weilbaecher, H. Kaneshima, E. J. Yee, and J. M. McCune, J. Exp. Med. 171: 1055 (1990).
R. B. Bankert, T. Umemoto, Y. Sugiyama, F. A. Chen, E. Repasky, and S. Yokota. Curr. Top. Microbiol Immunol 152: 201 (1989).
D. T. Purtilo, K. Falk, S. J. Pirrucello, H. Nakamine, K. Kleveland, J. R. David, M. Okano, Y. Yaguchi, W. G. Sanger, and K. W. Beisel, Int. J. Cancer 47: 510 (1991).
J. McClune, H. Kaneshima, J. Krowka, R. Namikawa, H.Outzen, B. Peault, L. Rabin, C. Shih, and E. Yee, Annu. Rev. Immunol 9: 399 (1991).
H. Tighe, G. Silverman, F. Kozin, R. Tucker, R. Gulizia, C. Peebles, M. Lotz, G. Rhodes, K. Machold, D. E. Mosier, and D. A. Carson, Eur. J. Immunol 20: 1843 (1990).
D. Nadal, B. Albini, C. Chen, E. Schlapfer, J. M. Bernstein, and P. L. Ogra, Int. Arch. Allergy Appl. Immunol 95: 341 (1991).
D. Nadal, B. Albini, E. Schlapfer, L. Brodsky, and P. L. Ogra, Clin. Exp. Immunol 85: 358 (1991).
D. E. Mosier, J. Clin. Immunol 10: 185 (1990).
K. W. Moore, P. Vieira, D. F. Fiorentino, M. L. Trounstine, T. A. Kahn, and T. R. Mossmann, Science 248: 1230 (1990).
C. D. Gregory, C. Dive, S. Henderson, C. A. Smith, G. T. Williams, J. Gordon, and A. B. Rickinson, Nature 349: 612 (1991).
G. C. Bosma, M. Fried, R. P. Custer, A. Carroll, D. M. Gibson, and M. J. Bosma, J. Exp. Med. 167: 1016 (1988).
D. E. Mosier, R. J. Gulizia, S. M. Bard, and D. B. Wilson, Nature 338: 211 (1989).
M. Brenan and C. R. Parish, J. Immunol Methods 74: 31 (1984).
C. Chen, D. Nadal, S. A. Cohen, E. Schlapfer, B. K. Mookerjee, A. Vladutiu, M. W. Stinson, P. L. Ogra, and B. Albini, Int. Arch. Allergy Appl Immunol 97: 295 (1992).
D. Nadal, B. Albini, E. Schlapfer, J. M. Bernstein, and P. L. Ogra, J. Gen. Virol 73: 113 (1992).
W. J. Murphy, S. K. Durum, and D. L. Longo, Proc. Natl Acad. Sci. USA 89: 4481 (1992).
C. Lue, H. Kiyono, K. Fujihashi, J. R. McGhee, and J. Mestecky, Regional Immunol 4: 86 (1992).
C. Chen, B. Albini, D. Nadal, B. K. Mookerjee, P. L. Ogra, M. W. Stinson, and S. A. Cohen, Amer. J. Pathol (Submitted).
C. Czerkinsky, Z. Moldoveanu, J. Mestecky, and L. A. Ouchterlony, J. Immunol Methods 115: 31 (1988).
C. E. Lewis, J. Immunol Methods 127: 51 (1990).
S. S. Williams, T. Umemoto, H. Kida, E. A. Repasky, and R. B. Bankert, J. Immunol, (in press, 1992).
Y. Shinkae, G. Rathburn, K.-P. Lam, E. M. Oltz, V. Steward, M. Mendelsohn, J. Charron, M. Datta, F. Young, A. M. Stall, and F. W. Alt, Cell 68: 855 (1992).
P. Mombaerts, J. Iacomini, R. S. Johnson, K. Herrup, S. Tonegawa, and V. E. Papaionnou, Cell 68: 869 (1992).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Albini, B. et al. (1995). Assessment of Engraftment and Function of Human Tonsillar and Blood Mononuclear Cells in Immunodeficient Mice. In: Mestecky, J., Russell, M.W., Jackson, S., Michalek, S.M., Tlaskalová-Hogenová, H., Šterzl, J. (eds) Advances in Mucosal Immunology. Advances in Experimental Medicine and Biology, vol 371. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1941-6_15
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1941-6_15
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5796-4
Online ISBN: 978-1-4615-1941-6
eBook Packages: Springer Book Archive