Abstract
There is evidence to assume a participation of an inducible NO-synthase (iNOS) in lipopolysaccharide (LPS)-induced shock (Thiemermann and Vane, 1990). Nitric oxide (NO) has been proposed as a mediator of hypotension in septic shock (Julou-Schaeffer et al., 1990; Kilbourn et al., 1990). The distribution of iNOS under LPS-treatment in vivo however has not been exactly localized. Nevertheless there is evidence for an expression of the inducible NO-synthase in a variety of cells including vascular smooth muscle cells (Fleming et al., 1991), macrophages (Green et al., 1990), neutrophils (McCall et al., 1989) and endothelial cells (Ohshima et al., 1992). To investigate the effect of iNOS in the vascular system and blood regulation we examined the expression of iNOS in the spleen and the heart of LPS-treated rats after the onset of the hypodynamic phase. The method used was NADPH-diaphorase-staining which selectively stained the different isoforms of NOS (Hope et al., 1991; Kobzik et al., 1993; Myatt et al., 1993). The differentation of iNOS endothelial and neuronal NOS was followed by comparison of the untreated control rats and the LPS-treated rats.
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Bloch, W., Dickneite, G., Krahwinkel, A., Dobers, F., Addicks, K. (1995). Induction of Nadph — Diaphorase — Activity with Endotoxin in Rat Heart and Spleen in Vivo. In: Weissman, B.A., Allon, N., Shapira, S. (eds) Biochemical, Pharmacological, and Clinical Aspects of Nitric Oxide. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1903-4_19
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DOI: https://doi.org/10.1007/978-1-4615-1903-4_19
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