Site Directed Mutagenesis of the Murine Coronavirus Spike Protein

Effects on Fusion
  • E. C. W. Bos
  • L. Heijnen
  • W. J. M. Spaan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)


Mutations were introduced in the transmembrane region of the spike protein of the murine coronavirus A59. The maturation of these mutant S proteins was not affected, they were all expressed at the cell surface, and became acylated, however some mutant S proteins did not induce cell-to-cell fusion. An I→K change in the middle of the predicted transmembrane (TM) anchor and mutation of the first three cysteine residues of the TM domain resulted in a fusion-negative phenotype. We propose a model by which these data can be explained.


Cysteine Residue Charged Residue Heptad Repeat Spike Protein Golgi Stack 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • E. C. W. Bos
    • 1
  • L. Heijnen
    • 1
  • W. J. M. Spaan
    • 1
  1. 1.Institute of Virology, Faculty of MedicineUniversity of LeidenThe Netherlands

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