Abstract
The genome of Lelystad virus (LV), a positive-strand RNA virus, is 15 kb in length and contains 8 open reading frames that encode putative viral proteins. Synthetic polypeptides of 15 to 17 amino acids were selected from the amino acid sequences of ORFs 2 to 7 and anti-peptide sera were raised in rabbits. Using these anti-peptide sera and porcine anti-LV serum, we identified three structural proteins and assigned their corresponding genes. Virions were found to contain a nucleocapsid protein of 15 kDa (N), an unglycosylated membrane protein of 18 kDa (M), and a glycosylated membrane protein of 25 kDa (E). The N protein is encoded by ORF7, the M protein is encoded by ORF6, and the E protein is encoded by ORF5.
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Keywords
- Infectious Bronchitis Virus
- Nucleocapsid Protein
- Replication Strategy
- CL2621 Cell
- Equine Arteritis Virus
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Wensvoort, G., Terpstra, C., Pol, J.M.A., Ter Laak, E.A., Bloemraad, M., de Kluyver, E.P., Kragten, C., van Buiten, L., den Besten, A., Wagenaar, F., Broekhuijsen, J.M., Moonen, P.L. J.M., Zetstra, T., de Boer, E.A., Tibben, H.J., de Jong, M.F., van t Veld, P., Groenland, G.J.R., van Gennep, J.A., Voets, M.Th., Verheijden, J.H.M., and Braamskamp, J. Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Vet Quart 1991;13:121–130.
Collins, J.E., Benfield, D.A., Christianson, W.T., Harris, L., Hennings, J.C., Shaw, D.P., Goyal, S.M., McCullough, S., Morrison, R.B., Joo, H.S., Gorcyca, D.E., Chladek, D.W. Isolation of swine infertility and respiratory syndrome virus (Isolate ATCC-VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J of Vet Diagn Invest 1992;4:117–126.
Wensvoort, G. Lelystad virus and the porcine epidemic abortion and respiratory syndrome. Vet Res 1993;24:117–124.
Meulenberg, J.J.M., Hulst, M.M., de Meijer, E.J., Moonen, P.L.J.M., den Besten, A., de Kluyver, E.P., Wensvoort, G., and Moormann,, R.J.M. Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS) is related to LDV and EAV Virology 1993;192:62–74.
Conzelmann, K.K., Visser, N., van Woensel, P., and Tiel, H.J. Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the Arterivirus group. Virology 1993; 193:329–339.
Meulenberg, J.J.M., de Meijer, and Moormann, R.J.M. Subgenomic RNAs of Lelystad virus contain a conserved junction sequence. J Gen Virol 1993;74:1697–1701.
Plagemann, P.G.W., and Moennig, V. Lactate dehydrogenase-elevating virus, equine arteritis virus, and simian hemorrhagic fever virus: a new group of positive-strand RNA viruses. Adv in Virus Res 1991;41:99–192.
Purchio, A.F., Larson, R. and Collet, M.S. Characterization of bovine viral diarrhea proteins. J Virol 1984;50:666–669.
Suter, M.A. Modified ELISA techniques for anti-hapten antibodies. J Immunol Meth 1982;53:103–108.
Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970;227:680–685.
Towbin, H., Staehelin, T., and Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitro cellulose sheets: Procedure and some applications. Proc Natl Acad Sci 1979;76:4350–4354.
Chen, Z., Kuo, L., Rowland, R.R.R., Even, C., Faaberg, K.S., and Plagemann, P.G.W. Sequences of 3’ end of genome and of 5’end of open reading frame la of Lactate dehydrogenase-elevating virus and common junction motifs between 5’leader and bodies of seven sugenomic mRNAs. J Gen Virol 1993;74:643–660.
Godeny, E.K., Speicher, D.W., and Brinton, M.A. Map location of lactate dehydrogenase-elevating virus (LDV) capsid protein (Vpl) gene. Virology 1990;177:768–771.
den Boon, J.A., Snijder, E.J., Chirnside, E.D., de Vries, A.A.F., Horzinek, M.C., and Spaan, W.J.M. Equine arteritis virus is not a togavirus but belongs to the coronavirus superfamily. J Virol 1991;65:2910–2920.
Brinton-Darnell, M., and Plagemann, P.G.W. Structure and chemical-physical characteristics of lactatedehydrogenase-elevating virus and its RNA. J Virol 1975;16:420–433.
Rottier, P., Brandenburg, D., Armstrong, J., van der Zeijst, B.A.M., and Warren, G. Assembly in vitro of a spanning membrane protein of the endoplasmic reticulum: The E1 glycoprotein of coronavirus mouse hepatitis virus A59. Proc Natl Acad Sci 1984;81:1421–1425.
Cavanagh, D., Davis, P.J., and Pappin, D.J.C. Coronavirus IBV glycopeptides: locational studies using proteases and saponin, a membrane permeabalizer. Virus Res 1986;4:145–156.
De Vries, A.A.F., chinside, E.D., Hozinek, M.C., and Rottier, P.J.M. Structural proteins of equine arteritis virus. J Virol 1992;66:6294–6303.
Nelson, E.A., Chrisopher-Hennings, J., Drew, T., Wensvoort, G., Collins, J.E., and Benfield, D.A. Differentiation of United states and european isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies. J of Clin Microbiol 1993;31: 3184–3189.
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Meulenberg, J.J.M., Besten, A.Pd., de Kluyver, E.P., Moormann, R.J.M., Schaaper, W.M.M., Wensvoort, G. (1995). Characterization of Structural Proteins of Lelystad Virus. In: Talbot, P.J., Levy, G.A. (eds) Corona- and Related Viruses. Advances in Experimental Medicine and Biology, vol 380. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1899-0_43
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DOI: https://doi.org/10.1007/978-1-4615-1899-0_43
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