MHV-3 Induced Prothrombinase is Encoded by MUSFIBLP
Previously, we demonstrated induction of a unique macrophage prothrombinase, PCA, in MHV-3 infected BALB/cJ mice. By immunologic screening, a clone representing PCA was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a flbrinogen-like protein. Six additional clones were isolated, and one clone, pl 1-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constituitively express musfiblp, but when infected with MHV-3, synthesized musfiblp-specific mRNA. musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrate when musfiblp was expressed from pl 1-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.
KeywordsEntire Code Region RK13 Cell Murine Hepatitis Virus Immunologic Screening Prothrombinase Activity
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