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MHV-3 Induced Prothrombinase is Encoded by MUSFIBLP

  • Rebecca L. Parr
  • Laisum Fung
  • Jeffrey Reneker
  • Nancy Myers-Mason
  • Julian L. Leibowitz
  • Gary Levy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

Previously, we demonstrated induction of a unique macrophage prothrombinase, PCA, in MHV-3 infected BALB/cJ mice. By immunologic screening, a clone representing PCA was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a flbrinogen-like protein. Six additional clones were isolated, and one clone, pl 1-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constituitively express musfiblp, but when infected with MHV-3, synthesized musfiblp-specific mRNA. musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrate when musfiblp was expressed from pl 1-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.

Keywords

Entire Code Region RK13 Cell Murine Hepatitis Virus Immunologic Screening Prothrombinase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Rebecca L. Parr
    • 1
  • Laisum Fung
    • 1
  • Jeffrey Reneker
    • 1
  • Nancy Myers-Mason
    • 3
  • Julian L. Leibowitz
    • 1
    • 2
  • Gary Levy
    • 3
  1. 1.Department of Pathology and Laboratory MedicineUSA
  2. 2.Department of Microbiology and Molecular GeneticsUniversity of Texas Health Sciences CenterHoustonUSA
  3. 3.Department of MedicineThe Toronto Hospital, University of TorontoCanada

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