MHV-3 Induced Prothrombinase is Encoded by MUSFIBLP

  • Rebecca L. Parr
  • Laisum Fung
  • Jeffrey Reneker
  • Nancy Myers-Mason
  • Julian L. Leibowitz
  • Gary Levy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)


Previously, we demonstrated induction of a unique macrophage prothrombinase, PCA, in MHV-3 infected BALB/cJ mice. By immunologic screening, a clone representing PCA was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a flbrinogen-like protein. Six additional clones were isolated, and one clone, pl 1-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constituitively express musfiblp, but when infected with MHV-3, synthesized musfiblp-specific mRNA. musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrate when musfiblp was expressed from pl 1-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.


Entire Code Region RK13 Cell Murine Hepatitis Virus Immunologic Screening Prothrombinase Activity 
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Rebecca L. Parr
    • 1
  • Laisum Fung
    • 1
  • Jeffrey Reneker
    • 1
  • Nancy Myers-Mason
    • 3
  • Julian L. Leibowitz
    • 1
    • 2
  • Gary Levy
    • 3
  1. 1.Department of Pathology and Laboratory MedicineUSA
  2. 2.Department of Microbiology and Molecular GeneticsUniversity of Texas Health Sciences CenterHoustonUSA
  3. 3.Department of MedicineThe Toronto Hospital, University of TorontoCanada

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