MHV-3 Induced Prothrombinase is Encoded by MUSFIBLP
Previously, we demonstrated induction of a unique macrophage prothrombinase, PCA, in MHV-3 infected BALB/cJ mice. By immunologic screening, a clone representing PCA was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a flbrinogen-like protein. Six additional clones were isolated, and one clone, pl 1-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constituitively express musfiblp, but when infected with MHV-3, synthesized musfiblp-specific mRNA. musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrate when musfiblp was expressed from pl 1-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.
KeywordsFormaldehyde Hepatitis Agarose Hydrochloride Polyacrylamide
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- 8.Sambrook, J., Fritsch, E.F., and Maniatis, T. In: Molecular Cloning: A Laboratory Manual, Second Edition (Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press) 1989.Google Scholar
- 11.Fung, L.S., Neil, G., Leibowitz, J., Cole, E.H., Chung, S., Crow, A., and Levy, G.A.. Monoclonal antibody analysis of a unique macrophage procoagulant activity induced by murine hepatitis virus strain 3 infection. J. Biol. Chem. 1991;226(3): 1789–1795.Google Scholar
- 12.Chung, S, Sinclair, S., Leibowitz, J, Skamene, E., Fung, L.S., and Levy, G.A. Cellular and metabolic requirements for induction of macrophage procoagulant activity by murine hepatitis virus strain 3 in vitro. J. Immunol. 1991;145:271–278.Google Scholar