Abstract
Retinal pigment epithelium (RPE) cells from the embryonic chick, maintained in culture, were incubated in the presence of [2-3H]-mannose and the oligosaccharide-lipids isolated and analyzed. After mild acid hydrolysis and reduction with NaBH4, the oligosac-charide pattern was examined by HPLC. The full array of intermediates of the dolichol pathway was observed with the glucose-containing species, Glc1, Glc2 and Glc3Man9GlcNAc2-P-P-dolichol, being the major oligosaccharide-lipids detected. Additional evidence supporting the formation of glucose-containing oligosaccharide-lipids by the RPE was obtained when analyzed by high pH anion exchange chromatography, comparing the products synthesized by the RPE with those formed by Madin-Darby canine kidney cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Kornfeld, R., and Kornfeld, S. (1985) Assembly of asparagine-linked oligosaccharides. Annu. Rev. Biochem. 54, 631–664.
Plantner, J. J., and Kean, E. L. (1988) The dolichol pathway in the retina: Oligosaccharide-lipid biosynthesis. Exp. Eye Res. 46, 785–800.
Kean, E. L. (1982) Activation by dolichol phosphate-mannose of the biosynthesis of N-acetylglucosaminylpyrophosphoryl polyprenols by the retina. J. Biol. Chem. 257: 7952–7954.
Kean, E. L. (1985) Stimulation by dolichol phosphate-mannose and phospholipids of the biosynthesis of N-acetylglucosaminyl pyrophosphoryl-dolichol. J. Biol. Chem. 260, 12561–12571.
Kean, E. L. (1987). Metabolic regulation of the initial reactions of the dolichol pathway: activation and deactivation of the biosynthesis of GlcNAc-P-P-dolichol. Chemica Scripta. 27, 127–134.
Kean, E. L., Rush, J. S., and Waechter, C. J. (1994) Activation of GlcNAc-P-P-dolichol synthesis is stereospecific and requires a saturated a-isoprene unit. Biochem. 33, 10508–10512.
Kean, E. L., Hara, S., and Lentrichia, B. B. (1981) Binding of neoglycoproteins by the chick pigment epithelial cell in culture. Vis. Res. 21, 133–135.
D’ Souza, C. D., Sharma, C. B., and Elbein, A. D. (1992) Biosynthesis of lipid-linked oligosaccharides. Anal. Biochem. 201, 211–217.
Prasad, A. V. K., Plantner, J. J., and Kean, E. L. (1992) Effect of enzymatic deglycosylation on the regenerability of bovine rhodopsin. Exp. Eye Res. 54, 913–920.
Pan, Y. T., and Elbein, A. D. (1990) Control of N-linked oligosaccharide synthesis: cellular levels of dolichol phosphate are not the only regulatory factor. Biochem. 29, 8077–8084.
Kean, E. L., and DeBrakeleer, D. J.(1986) Stimulation by dolichol-P-mannose of GlcNAc-lipid biosynthesis by membranes from class E Thy-1 negative mutant mouse lymphoma cells which are defective in dolichol-P-mannose biosynthesis. Arch. Biochem. Biophys. 250, 146–152.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Kean, E.L., Niu, N. (1995). The Dolichol Pathway in the Retinal Pigment Epithelium of the Embryonic Chick. In: Anderson, R.E., LaVail, M.M., Hollyfield, J.G. (eds) Degenerative Diseases of the Retina. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1897-6_17
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1897-6_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5774-2
Online ISBN: 978-1-4615-1897-6
eBook Packages: Springer Book Archive