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The Dolichol Pathway in the Retinal Pigment Epithelium of the Embryonic Chick

  • Edward L. Kean
  • Naiqian Niu

Abstract

Retinal pigment epithelium (RPE) cells from the embryonic chick, maintained in culture, were incubated in the presence of [2-3H]-mannose and the oligosaccharide-lipids isolated and analyzed. After mild acid hydrolysis and reduction with NaBH4, the oligosac-charide pattern was examined by HPLC. The full array of intermediates of the dolichol pathway was observed with the glucose-containing species, Glc1, Glc2 and Glc3Man9GlcNAc2-P-P-dolichol, being the major oligosaccharide-lipids detected. Additional evidence supporting the formation of glucose-containing oligosaccharide-lipids by the RPE was obtained when analyzed by high pH anion exchange chromatography, comparing the products synthesized by the RPE with those formed by Madin-Darby canine kidney cells.

Keywords

Retinal Pigment Epithelium MDCK Cell Retinal Pigment Epithelium Cell Embryonic Chick Pulse Amperometric Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Edward L. Kean
    • 1
  • Naiqian Niu
    • 1
  1. 1.Department of OphthalmologyCase Western Reserve UniversityClevelandUSA

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