The Dolichol Pathway in the Retinal Pigment Epithelium of the Embryonic Chick
Retinal pigment epithelium (RPE) cells from the embryonic chick, maintained in culture, were incubated in the presence of [2-3H]-mannose and the oligosaccharide-lipids isolated and analyzed. After mild acid hydrolysis and reduction with NaBH4, the oligosac-charide pattern was examined by HPLC. The full array of intermediates of the dolichol pathway was observed with the glucose-containing species, Glc1, Glc2 and Glc3Man9GlcNAc2-P-P-dolichol, being the major oligosaccharide-lipids detected. Additional evidence supporting the formation of glucose-containing oligosaccharide-lipids by the RPE was obtained when analyzed by high pH anion exchange chromatography, comparing the products synthesized by the RPE with those formed by Madin-Darby canine kidney cells.
KeywordsRetinal Pigment Epithelium MDCK Cell Retinal Pigment Epithelium Cell Embryonic Chick Pulse Amperometric Detection
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