Peptides from Rhodopsin Induce Experimental Autoimmune Uveoretinitis in Lewis Rats
Experimental autoimmune uveoretinitis (EAU) is an organ-specific, T cell mediated autoimmune disease, which is characterized by destruction of the photoreceptor cells of the retina. EAU serves as a model for human intraocular inflammatory diseases, like uveitis, which are major causes of visual impairment. The animal disease can be induced by immunization with certain retinal proteins. The antigens used in most studies for EAU induction have been S-antigen (arrestin) and interphotoreceptor retinoid binding protein (IRBP)(Gery, Michizuki and Nussenblatt, 1986). We, and other investigators, have reported that rhodopsin is similarly uveitogenic in rats, guinea pigs, and in monkeys (Adamus, et al., 1992; Marak, et al., 1980; Meyers-Elliott and Sumner, 1982; Moticka and Adamus, 1991; Schalken, et al., 1988b; Schalken, et al., 1989; Wong, et. al., 1977). Rhodopsin is the major protein of photoreceptor cells and has a molecular weight of 40,000 daltons. It is the photoreceptor protein that initiates the visual transduction process (Hargrave and McDowell, 1993).
KeywordsPhotoreceptor Cell Bovine Rhodopsin Experimental Autoimmune Uveitis Experimental Autoimmune Uveoretinitis Interphotoreceptor Retinoid Binding Protein
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- Adamus, G., Dugger, D., Arendt, A., and Hargrave, P.A. (1994) Characterization of an immunopathogenic epitope in rhodopsin that induces experimental autoimmune uveitis. Reg. Immunol. 6: 44–46.Google Scholar
- Gery, I., Michizuki, M., and Nussenblatt, R.B. Retinal specific antigens and immunopathogenic process they provoke. Vol. 5 of Progress in Retinal Research. Oxford: Pergamon Press, 1986. 75–109.Google Scholar
- Lipham, W.J., Redmond, T.M., Takahashi, H., Berzofsky, J.A., Wiggert, B., Chader, G.J., and Gery, I. (1991) Recognition of peptides that are immunopathogenic but cryptic. Mechanisms that allow lymphocytes sensitized against cryptic peptides to initiate pathogenic autoimmune processes. J. Immunol. 146: 3757–3762.PubMedGoogle Scholar
- Schalken, J.J., VanVugh, A.H.M., Winkens, HJ., Bovee-Geurts, P.H.M., DeGrip, W.J., and Broekhuyse, R.M. (1988a) Experimental autoimmune uveoretinitis in rats induced by rod visual pigment: Rhodopsin is more pathogenic than opsin. Graefe’ s Arch. Clin. Exp. Ophthalmol. 226: 255–261.CrossRefGoogle Scholar
- Wong, V.G., Green, W.R., and McMaster, P.R.B. (1977) Rhodopsin and blindness. Tr. Am. Ophthal Soc. 75: 272–284.Google Scholar