Fractionation of Interphotoreceptor Matrix Metalloproteinases
Metalloproteinases (MPs) present in the interphotoreceptor matrix (IPM) may play a role in both the maintenance of normal retinal homeostasis and the progression of various degenerative retinal diseases. We wished to extend our previous studies of these enzymes and begin their characterization by first obtaining them in purified form.
IPM was obtained from fresh bovine eyes. The material was fractionated by a combination of gel filtration, DEAE ion exchange and gelatin- affinity chromatographies. Fractions were monitored by SDS-PAGE carried out on both plain gels and those preloaded with gelatin or casein (zymograms) and by quantitative analysis for enzyme activity Combination of the procedures described above provided a partial fractionation of the MPs as detected by zymography. A previously unrecognized 52 kDa caseinase was also revealed, possibly identical with MMP-3 (stromelysin).
KeywordsRetinal Pigment Epithelium Trabecular Meshwork Proliferative Vitreoretinopathy Interphotoreceptor Retinoid Binding Protein Degenerative Retinal Disease
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