Abstract
The effects of the vasoactive peptide angiotensin II (AII) on contractility and excitation-contraction coupling in isolated adult rabbit ventricular myocytes were investigated. In most ventricular myocytes, AII (10-8 M) induced a significant increase in fractional shortening which was not associated with an increase in the calcium transient measured with indo-1. AII did increase the intracellular pH by approximately 0.2 5 pH units coincident with the positive inotropic effect. Effects of AII on pH and contractility were blocked by inhibitors of Na+/H+ exchange. AII also increased the rate of pHi recovery from intracellular acidosis at pHi values above 6.9. AII was shown not to affect the L-type inward calcium current. However, in an occasional cell, AII was observed to cause a slight increase in the calcium transient. We hypothesize that this response may reflect an increase of calcium influx on the sodium calcium exchanger, as a consequence of an increase in subsarcolemmal sodium concentration resulting from enhanced Na+-H+ exchange.
Keywords
- Ventricular Myocytes
- Calcium Transient
- Positive Inotropic Effect
- Intracellular Acidosis
- Sodium Calcium Exchanger
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© 1995 Springer Science+Business Media New York
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Barry, W.H., Matsui, H., Bridge, J.H.B., Spitzer, K.W. (1995). Excitation-Contraction Coupling in Ventricular Myocytes: Effects of Angiotensin II. In: Sideman, S., Beyar, R. (eds) Molecular and Subcellular Cardiology. Advances in Experimental Medicine and Biology, vol 382. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1893-8_4
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DOI: https://doi.org/10.1007/978-1-4615-1893-8_4
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