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Regulation of IL-4 and IL-5 Secretion by Histamine and PGE2

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Immunobiology of Proteins and Peptides VIII

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 383))

Summary

This study was designed to study the effects of autacoids on IL-4 and IL-5 secretion. IL-4 and IL-5 are secreted by TH2 cells. TH2 cells were generated by the culture of peripheral blood lymphocytes from atopic individuals in the presence of ragweed or dustmite antigen. The cloned TH2 lymphocytes were then stimulated with PMA (10 ng/ml) and a-CD3 (50 ng/ml) in the presence and absence of histamine (104-10-8M) and PGE2 (10-6-10-8M) for 48 hours. Other cAMP elevating agents were used as control. The supernatants were then assayed for the presence of IL-4 and IL-5 by ELISA. Both histamine and PGE2 suppressed the secretion of IL-4 in a dose dependent manner. Other cAMP elevating agents did not affect IL-4 secretion. In contrast, histamine upregulated the secretion of IL-5, whereas the effects of PGE2 on IL-5 secretion were not conclusive. Chloride channels have been implicated in the secretory processes. The effects of a chloride channel blocker, DIDS, were studied on histamine-induced suppression of IL-4 secretion. DIDS (10-7-10-’2M) abrogated the inhibitory effects of histamine on IL-4 secretion. The observations suggest that histamine may inhibit IL-4 secretion via activation of chloride channels.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Creighton University Health Sciences Center, Omaha, Nebraska, 68178, USA

    Manzoor M. Khan

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  1. Manzoor M. Khan
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Editors and Affiliations

  1. Baylor College of Medicine, Houston, Texas, USA

    M. Zouhair Atassi

  2. Univax Biologics, Inc., Rockville, Maryland, USA

    Garvin S. Bixler Jr.

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© 1995 Springer Science+Business Media New York

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Khan, M.M. (1995). Regulation of IL-4 and IL-5 Secretion by Histamine and PGE2 . In: Atassi, M.Z., Bixler, G.S. (eds) Immunobiology of Proteins and Peptides VIII. Advances in Experimental Medicine and Biology, vol 383. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1891-4_5

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  • DOI: https://doi.org/10.1007/978-1-4615-1891-4_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5771-1

  • Online ISBN: 978-1-4615-1891-4

  • eBook Packages: Springer Book Archive

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