Abstract
Two cDNA clones, BMR6 and BMR78, which detect transcripts of genes that increase in abundance in the roots of Hordeum vulgare cv. ‘Galleon’ during colonisation by the vesicular-arbuscular mycorrhizal fungus Glomus intraradices have recently been isolated. Sequence analysis indicated that BMR78 is a partial clone of a H+ - ATPase gene. A putative fiinction for the gene corresponding to BMR6 was not ascertained.
The genomic DNA sequences which hybridise to BMR6 and BMR78 were mapped to barley chromosomes 6H and 2H respectively, by segregation analysis of restriction fragment length polymorphisms (RFLP’s) in two doubled haploid mapping populations. The results were confirmed by hybridisation analysis of wheat-barley chromosome addition lines. BMR78 maps to a region of the barley genome which contains a putative nematode resistance gene.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Baon J.B., Smith S.E. and Alston A.M. (1994). Mycorrhizal responses of barley cultivars differing in P efficiency. Plant and Soil 157: 97–105.
Boutry M., Michelet B. and Goffeau A. (1989). Molecular cloning of the family of genes encoding a protein homologous to plasma membrane H+ - translocating ATPases. Biochem. Biophys. Res. Comm 162: 567–574
Ewing N.N., Wimmers L.E., Meyer DJ., Chetelat R.T. and Bennett A.B. (1990). Molecular cloning of tomato plasma membrane H+-ATPase. Plant Physiol 94: 1874–1881
Feinberg A.P. and Vogelstein B. (1983). Atechnique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13
Gianinazzi-Pearson V., Smith S.E., Gianinazzi S. and Smith F.A. (1991). V. Is H+ - ATPase a component of ATP-hydrolysing enzyme activities in plant-fungus interfaces? New Phytol 117: 61–74
Guidet F., Rogowski P., Taylor C., Weining S. and Langridge P. (1991). Cloning and characterisation of a new rye-specific repeated sequence. Genome 34: 81–87
Harley J. L. and Smith S.E. (1983). Mycorrhizal Symbiosis. London, New York: Academic Press.
Harper J.F., Manney L. and Sussman M.R. (1994). The plasma membrane H+ - ATPase gene family in Arabidopsis : genomic sequence of AHA10 which is primarily expressed in developing seeds. Mol. Gen. Genet 244: 572–587
Houlne G. and Boutry M. (1994). Identification of an Arabidopsis thaliana gene encoding a plasma membrane H+ - ATPase whose expression is restricted to anther tissues. Plant Journal 5:311–317
Islam A.K.M.R., Shepherd K.W. and Sparrow D.H.B. (1981). Isolation and characterisation of euplasmic wheat-barley chromosome addition lines. Heredity 46: 161–174
Koide, R.T. and Schreiner, R.P. (1992). Regulation of the vesicular-arbuscular symbiosis. Ann. Rev. Plant Physiol Mol. Biol 43: 557–81
Lander E.S, Green J., Abrahamson A., Baarlow M.J., and Daly M.J. (1987). MAPMAKER: an interactive package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181
Maniatis T., Fritsch E.F. and Sambrook J. (1982). Molecular Cloning: A Laboratory Manual. Cold Spring Harbour Laboratory. Cold Spring Harbour, New York.
Morton J.B. and Benny G.L. (1992). Revised classification of arbuscular mycorrhizal fungi (Zygomycetes): A new order, Glomales, two new suborders, Glominae and Gigasporinae, and two new families, Acaulosporaceae and Gigasporaceae. Mycotaxon 37: 471–491
Ralfalski J.A. and Tingey S.V. (1993). Genetic diagnostics in plant breeding: RAPD’s, microsatellites and machines. Trends in Genetics 9: 275–279
Smith S.E. and Gianinazzi-Pearson V (1988). Physiological interactions between symbionts in vesicular-arbuscular mycorrhizal plants. Ann. Rev. Plant Physiol 39: 221–244
Smith S.E. and Smith F.A. (1990). Structure and function of the interfaces in biotrophic symbioses as they relate to nutrient transport. New Phytol 114: 1–38
Smith S.E., Robson A.D. and Abbott K. (1992). The involvement of mycorrhizas in assessment of genetically dependant efficiency of nutrient uptake and use. Plant and Soil 146: 169–179
Williams K.J., Fisher J.M. and Langridge P. (1994). Identification of RFLP markers linked to the cereal cyst nematode resistance gene (Cre) in wheat. Theor. Appl Genet 89: 927–930
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Murphy, P.J., Karakousis, A., Smith, S.E., Langridge, P. (1995). Cloning Functional Endomycorrhiza Genes. In: Stocchi, V., Bonfante, P., Nuti, M. (eds) Biotechnology of Ectomycorrhizae. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1889-1_7
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1889-1_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5770-4
Online ISBN: 978-1-4615-1889-1
eBook Packages: Springer Book Archive