Cloning Functional Endomycorrhiza Genes
Two cDNA clones, BMR6 and BMR78, which detect transcripts of genes that increase in abundance in the roots of Hordeum vulgare cv. ‘Galleon’ during colonisation by the vesicular-arbuscular mycorrhizal fungus Glomus intraradices have recently been isolated. Sequence analysis indicated that BMR78 is a partial clone of a H+ - ATPase gene. A putative fiinction for the gene corresponding to BMR6 was not ascertained.
The genomic DNA sequences which hybridise to BMR6 and BMR78 were mapped to barley chromosomes 6H and 2H respectively, by segregation analysis of restriction fragment length polymorphisms (RFLP’s) in two doubled haploid mapping populations. The results were confirmed by hybridisation analysis of wheat-barley chromosome addition lines. BMR78 maps to a region of the barley genome which contains a putative nematode resistance gene.
KeywordsPhosphorus Agarose Electrophoresis Expense Butanol
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