Abstract
Most secreted and cell surface proteins are glycosylated. There are three principal ways by which glycans may be attached to the protein: via the amino acid sequon of Asn-Xxx-Ser/Thr for N-glycosylation, the amino acids Ser/Thr for O-glycosylation and the C terminal amino acid in the GPI anchor of membrane bound glycoproteins. Typically a glycoprotein has greater than three glycan sites, each site containing between 10 and 30 structures. The presence of this many glycans on a single protein gives rise to the concept of glycoforms. Glycans on the protein can profoundly affect the 3-D structure of the protein and its biological function.1 The study of these glycans is therefore important whether one is involved in glycoprotein basic research, structure/function relationships, recombinant glycoprotein production or recombinant protein quality control.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Varki, A., Glycobiology, 1993, 3, pp 165–183.
Parekh, R., Biochemistry, 1989, 28, pp 7670, 7644 and 7662.
Goochee, C., Bio/Technology, 1990, 8, pp 421.
Lacore, Circulation, 1988, 77, pp 906–914.
Curling, E. and Bull, J., Biochem J, 1990, 272, pp 333.
Maiorella, B., BioTechnology, March 1993, 11, pp 387–392.
Patel, T., Biochem J, 1992, 285, pp 839–845.
Oxford GlycoSystems, Technical Bulletin No. 7, 1991
Thall, A., et al. Biochemistry, 1990, 29, pp 3959
Ashford, D,. et al. Eur. J. Biochem., 1987, 166, pp 311
Kobata, A,. Biology of Carbohydrates, Wiley, 1984, 2, pp 87
Tsuda, Eur J Biochem, 1990, 188 pp 405.
Takeuchi, PNAS, 1986, pp 7819.
Parekh, R. B., Biochemistry, 1989, 28, pp 7670.
Gribben, The Lancet, 1990, pp 434 to 437.
Damm, J.B.L., et al. Glycoconjugate Journal 1987, 4, 129
Nuck, R., et al. Glycoconjugate Journal 1990, 7, 279
Umemoto, J., J. Biol. Chem., 1977, 252, 8609
Carlsson, D. M., J. Biol. Chem., 1968, 243, 616
Fukuda, M., Meth Enzymol., 1989, 179, 17
Likhosherstov, L. M., Carbohydr. Res., 1988, 57, 785
Takasaki, S., Meth Enzymol., 1982, 83, 263
Patel, T., et al. Biochemistry, 1993, 32, 679–683
Oxford GlycoSystems, GlycoPrep Update No. 1, 1991
Hase, S., et al., J. Biochem., 1984, 95, 197
Rothenberg, B. E., Proc. Natl. Acad. Sci., 1993, 90, 11939
Dwek, R. A. et al., Ann Rev Biochem., 1993, 62, 65
Edge, C., et al., Proc. Natl. Acad. Sci., 1992, 89, 6338
Prime, S., et al., OGS Application Note IAN-01, 1993
Dearnley, J., et al., OGS poster titled ‘The RAAM Enzyme Array for Sialylated N-Glycans’, 1994
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Dennis, R.P. (1995). A Review of the Biological Significance of Carbohydrates on Glycoproteins and Methods for their Analysis. In: Alavi, A., Axford, J.S. (eds) Glycoimmunology. Advances in Experimental Medicine and Biology, vol 376. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1885-3_1
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1885-3_1
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5768-1
Online ISBN: 978-1-4615-1885-3
eBook Packages: Springer Book Archive