Abstract
Damage to structural or regulatory genes in germ cell DNA is expected to produce abnormalities in the abundance or structure of the proteins whose synthesis is controlled by those genes in subsequent progeny. Detection of such protein abnormalities could be useful for monitoring mutation frequencies in the offspring of exposed populations, for the molecular characterization of DNA damage caused by different environmental or occupational exposures (e.g., ionizing radiation, toxic chemicals), and for the correlation of such damage with deleterious effects in subsequent generations. The biochemical specific locus test, in which the activities of approximately 30 different proteins can be monitored by one-dimensional electrophoresis, has shown that heritable protein effects can be detected in mice sired by chemically exposed males (Johnson and Lewis, 1981).
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References
Anderson, N.G., and Anderson, N.L., 1978a, Analytical techniques for cell fractions. XXI. Two-dimensional analysis of serum and tissue proteins: Multiple isoelectric focusing, Analyt. Biochem. 85:331.
Anderson, N.L. and Anderson, N.G., 1978b, Analytical techniques for cell fractions. XXI. Two-dimensional analysis of serum and tissue proteins: Multiple gradient slab-gel electrophoresis, Analyt. Biochem. 85:341.
Anderson, N.L., Taylor, J., Scandora, A.E., Jr., Coulter, B.P., and Anderson, N.G., 1981, The TYCHO system for computer analysis of two-dimensional gel electrophoresis patterns, Clin. Chem. 27:1807.
Celis, J.E. and Bravo, R., 1984, “Two-Dimensional Gel Electrophoresis of Proteins,” Academic Press, New York.
Ehling, U.H., 1978, Specific-locus mutations in mice, in: “Chemical Mutagens: Principles and Methods for Their Detection,” A. Hollaender and F.J. deSerres, eds., Plenum Press, New York.
Giometti, C.S., Gemmell, M.A., Nance, S.L., Tollaksen, S.L., and Taylor, J., 1987, Detection of heritable mutations as quantitative changes in protein expression, J. Biol. Chem. 262:12764.
Giometti, C.S., Gemmell, M.A., Tollaksen, S.L., and Grahn, D., 1988, Heritable protein variants induced by exposure to ethylnitrosourea: Heritability, subcellular location, and tissue distribution, Mut. Res. 202:9.
Giometti, C.S., Gemmell, M.A., Taylor, J., Tollaksen, S.L., Angeletti, R., and Gluecksohn-Waelsch, S., 1992a, Evidence for regulatory genes on mouse chromosome 7 that affect the quantitative expression of proteins in the fetal and newborn liver, Proc. Natl. Acad. Sci. USA 89:2448.
Giometti, C.S., Taylor, J., and Tollaksen, S.L., 1992b, Mouse liver protein database: A catalog of proteins detected by two-dimensional gel electrophoresis, Electrophoresis (in press).
Giometti, C.S., and Taylor, J., 1991, The application of two-dimensional electrophoresis to mutation studies, in: “Advances in Electrophoresis,” M.J. Dunn and B.J. Radola, eds., VCH Verlagsgesellschaft, New York.
Giometti, C.S., Tollaksen, S.L., Gemmell, M.A., Taylor, J., Hawes, N., and Roderick, T., 1990, The analysis of recessive lethal mutations in mice by using two-dimensional gel electrophoresis of liver proteins, Mut. Res. 242:47.
Johnson, F.M. and Lewis, S.E., 1981, Electrophoretically detected germinal mutations induced in the mouse by ethylnitrosourea, Proc. Natl. Acad. Sci. USA 78:3138.
Russell, W.L., Kelly, E.M., and Hunsicker, P.R., 1979, Specific locus test shows ethylnitrosourea to be the most potent mutagen in the mouse, Proc. Natl. Acad. Sci. USA 76:5818.
Taylor, J., Anderson, N.L., and Anderson, N.G., 1981, A computerized system for matching and stretching two-dimensional gel patterns represented by parameter lists, in: “Electrophoresis’ 81,” R. Allen and P. Arnaud, eds., Walter de Gruyter and Co., New York.
Taylor, J., and Giometti, C.S., 1992, Use of principal components analysis for mutation detection with two-dimensional electrophoresis protein separations, Electrophoresis 13:162.
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Giometti, C.S. (1994). Two-Dimensional Electrophoresis of Proteins: Detection and Characterization of Male-Mediated Genotoxicity. In: Olshan, A.F., Mattison, D.R. (eds) Male-Mediated Developmental Toxicity. Reproductive Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1877-8_32
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DOI: https://doi.org/10.1007/978-1-4615-1877-8_32
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