Two-Dimensional Electrophoresis of Proteins: Detection and Characterization of Male-Mediated Genotoxicity
Damage to structural or regulatory genes in germ cell DNA is expected to produce abnormalities in the abundance or structure of the proteins whose synthesis is controlled by those genes in subsequent progeny. Detection of such protein abnormalities could be useful for monitoring mutation frequencies in the offspring of exposed populations, for the molecular characterization of DNA damage caused by different environmental or occupational exposures (e.g., ionizing radiation, toxic chemicals), and for the correlation of such damage with deleterious effects in subsequent generations. The biochemical specific locus test, in which the activities of approximately 30 different proteins can be monitored by one-dimensional electrophoresis, has shown that heritable protein effects can be detected in mice sired by chemically exposed males (Johnson and Lewis, 1981).
KeywordsToxicity Urea Electrophoresis Germinal Polyacrylamide
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