Aspergillus Niger Var. Macrosporus Proteinase B. cDNA Cloning, Expression, and Activation of the Proenzyme
Filamentous fungus Aspergillus niger var. macrosporus produces two types of extracellular acid endoproteinases, proteinases A and B, commercially called proctases A and B, respectively (1,2). Proteinase A is a non-pepsin-type acid proteinase (1–7), whereas proteinase B has properties of pepsin-type aspartic proteinase. Proteinase B has a molecular mass of about 35 kDa and is inhibited by specific inhibitors of aspartic proteinases such as pepstatin, diazoacetyl-DL-norleucine methyl ester in the presence of cupric ions, and 1,2-epoxy-3-(p-nitrophenoxy)propane (4,8).
KeywordsAspergillus Niger Mature Form Aspartic Proteinase Acid Proteinase Tryptic Fragment
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