Aspergillus Niger Var. Macrosporus Proteinase B. cDNA Cloning, Expression, and Activation of the Proenzyme

  • Hideshi Inoue
  • Jing-Fang Lu
  • Senarath B. P. Athauda
  • Kwang-Hoon Kong
  • Takaharu Hayashi
  • Takao Kimura
  • Osamu Makabe
  • Kenji Takahashi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 362)

Abstract

Filamentous fungus Aspergillus niger var. macrosporus produces two types of extracellular acid endoproteinases, proteinases A and B, commercially called proctases A and B, respectively (1,2). Proteinase A is a non-pepsin-type acid proteinase (1–7), whereas proteinase B has properties of pepsin-type aspartic proteinase. Proteinase B has a molecular mass of about 35 kDa and is inhibited by specific inhibitors of aspartic proteinases such as pepstatin, diazoacetyl-DL-norleucine methyl ester in the presence of cupric ions, and 1,2-epoxy-3-(p-nitrophenoxy)propane (4,8).

Keywords

Urea Propane Polypeptide Methionine Gelatin 

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Hideshi Inoue
    • 1
  • Jing-Fang Lu
    • 1
  • Senarath B. P. Athauda
    • 1
  • Kwang-Hoon Kong
    • 1
  • Takaharu Hayashi
    • 1
  • Takao Kimura
    • 2
  • Osamu Makabe
    • 2
  • Kenji Takahashi
    • 1
  1. 1.Department of Biophysics and Biochemistry Faculty of ScienceUniversity of TokyoTokyoJapan
  2. 2.Meiji Seika, Ltd.YokohamaJapan

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