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Non-Mammalian Vertebrate Pepsinogens and Pepsins: Isolation and Characterization

  • Chapter
Aspartic Proteinases

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 362))

Abstract

Pepsinogen, the precursor of pepsin, is secreted from the gastric mucosa into the gastric lumen, where it is autocatalytically activated to pepsin under acidic conditions. It is classified into three major types, that is, pepsinogen A, pepsinogen C (or progastricsin), and prochymosin (or neonatal pepsinogen). Further, pepsinogens, especially pepsinogens A, are often composed of several isozymogens. So far, a number of pepsinogens were purified from various sources, characterized and sequenced at the protein level and/or DNA level. Thus the complete amino acid sequences of mammalian pepsinogens are known for human (1), monkey (2), porcine (3–6), and rabbit (7) pepsinogens A, human (8), monkey (9), rat (10) and guinea pig (11) pepsinogens C, and bovine prochymosin (12).

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References

  1. K. Sogawa, Y. Fujii-Kuriyama, Y. Mizukami, Y. Ichihara and K. Takahashi, Primary Structure of Human Pepsinogen Gene, J. Biol. Chem. 258: 5306–5311 (1983).

    PubMed  CAS  Google Scholar 

  2. T. Kageyama and K. Takahashi, The Complete Amino Acid Sequence of Monkey Pepsinogen A, J. Biol Chem. 261: 4395–4405 (1986).

    PubMed  CAS  Google Scholar 

  3. J. Tang, P. Sepulveda, J. Marciniszyn Jr., K. C. S. Chen, W-Y. Huang, N. Tao, D. Liu and J. P. Lanier, Amino-Acid Sequence of Porcine Pepsin, Proc. Natl. Acad. Sci U. S. A. 70: 3437–3439 (1973).

    Article  PubMed  CAS  Google Scholar 

  4. E. B. Ong and G. E. Perlmann, The Amino-Terminal Sequence of Porcine Pepsinogen, J. Biol. Chem. 243:6104–6109 (1968).

    PubMed  CAS  Google Scholar 

  5. V. B. Pederson and B. Foltmann, The Amino Acid Sequence of A Hitherto Unobserved Segment from Porcine Pepsinogen Preceding the N-Terminus of Pepsin, FEBS Lett. 35: 255–256 (1973).

    Article  Google Scholar 

  6. V. M. Stepanov, L. A. Baratova, I. B. Pugacheva, L. P. Belyanova, L. P. Revina and E. A. Timokhina, N-Terminal Sequence of Swine Pepsinogen and Pepsin. The Site of Pepsinogn Activation, Biochem. Biophys. Res. Commun. 54: 1164–1170 (1973).

    Article  PubMed  CAS  Google Scholar 

  7. T. Kageyama, K. Tanabe and O. Koiwai, Structure and Development of Rabbit Pepsinogens. Stage-Specific Zymogens, Nucleotide Sequence of cDNAs, Molecular Evolution, and Gene Expression during Development, J. Biol. Chem. 265: 17031–17038 (1990).

    PubMed  CAS  Google Scholar 

  8. T. Hayano, K. Sogawa, Y. Ichihara, Y. Fujii-Kuriyama and K. Takahashi, Primary Structure of Human Pepsinogen C Gene, J. Biol. Chem. 263: 1382–1385 (1988).

    PubMed  CAS  Google Scholar 

  9. T. Kageyama and K. Takahashi, The Complete Amino Acid Sequence of Monkey Progastricsin, J. Biol. Chem. 261: 4406–4419 (1986).

    PubMed  CAS  Google Scholar 

  10. T. Ishihara, Y. Ichihara, T. Hayano. I. Katsura, K. Sogwa, Y Fujii-Kuriyama and K. Takahashi, Primary Strucutre and Transcriptional Regulation of Rat Pepsinogen C. Gene, J. Biol. Chem. 264: 10193–10199(1989).

    Google Scholar 

  11. T. Kageyama. M. Ichinose, S. Tsukada, K. Miki, K. Kurokawa, O. Koiwai, M. Tanji, E. Yakabe, S. B. P. Athauda and K. Takahashi, Gastric Prochathepsin E and Progastricsin from Guinea Pig. Purification, Molecular Cloning of cDNAs, and Characterization of Enzymatic Properties, with Special Reference to Procathepsin E, J. Biol. Chem. 267: 16450–16459 (1992).

    PubMed  CAS  Google Scholar 

  12. B. Foltmann, V. B. Pedersen, H. Jacobsen. D. Kauffman and G. Wybrandt, The Complete Amino Acid Sequence of Prochymosin, Proc. Natl Acad. Sci. U. S. A. 74: 2321–2324 (1977).

    Article  PubMed  CAS  Google Scholar 

  13. M. Baudyš and V. Kostka, Covalent Structure of Chicken Pepsinogen, Eur. J. Biochem. 136: 89–99 (1983).

    Article  PubMed  Google Scholar 

  14. K. Hayashi, K. Agata, M. Mochii, S. Yasugi, G. Eguchi and T. Mizuno, Molecular Cloning and the Nucleotide Sequence of cDNA for Embryonic Chicken Pepsinogen: Phylogenetic Relationship with Prochymosin, J. Biochem. 103: 290–296 (1988).

    PubMed  CAS  Google Scholar 

  15. M. Tanji, T. Kageyama and K. Takahashi, Tuna Pepsinogens and Pepsins. Purification, Characterization and Amino-Terminal Sequences, Eur. J. Biochem. 177: 251–259 (1988).

    Article  PubMed  CAS  Google Scholar 

  16. Z. Bohak, Purification and Characterization of Chicken Pepsinogen and Chicken Pepsin, J. Biol Chem. 244: 4638–4648 (1969).

    PubMed  CAS  Google Scholar 

  17. E. Yakabe, M. Tanji, M. Ichinose, S. Goto, K. Miki, K. Kurokawa, H. Ito, T. Kageyama and K. Takahashi, Purification, Characterization, and Amino Acid Sequences of Pepsinogens and Pepsins from the Esophageal Mucosa of Bullfrog (Rana catesbeiana), J. Biol Chem. 266: 22436–22443 (1991).

    PubMed  CAS  Google Scholar 

  18. I. M. Samloff, Slow Moving Protease and the Seven Pepsinogens. Electrophoretic Demonstration of the Existence of Eight Proteolytic Fractions in Human Gastric Mucosa, Gastroenterology 57: 659–669 (1969).

    PubMed  CAS  Google Scholar 

  19. S. B. P. Athauda, M. Tanji, T. Kageyama and K. Takahashi, A Comparative Study on the NH2-Terminal Amino Acid Sequences and Some Other Properties of Six Isozymic Forms of Human Pepsinogens and Pepsins, J. Biol Chem. 106: 920–927 (1989).

    CAS  Google Scholar 

  20. T. Kageyama and K. Takahashi, Pepsinogen C and Pepsin C from Gastric Mucosa of Japanese Monkey, J. Biochem. 80: 983–992 (1976).

    PubMed  CAS  Google Scholar 

  21. T. Kageyama and K. Takahashi, Pepsinogens and Pepsins from Gastric Mucosa of Japanese Monkey. Punfication and Characterization, J. Biochem. 79: 455–468 (1976).

    PubMed  CAS  Google Scholar 

  22. K. Takahashi and T. Kageyama, Multiplicity and Intermediates of the Activation Mechanism of Zymogens of Gastric Aspartic Proteinases, in: “Aspartic Proteinases and Their Inhibitors”, V. Kostka, ed., pp.265–282, Walter de Gruyter, Berlin and New York (1985).

    Google Scholar 

  23. O. Matsuzaki and K. Takahashi, Improved Purification of Slow Moving Protease from Human Gastric Mucosa and Its Action on the B Chain of Oxidized Bovine Insulin, Biomed Res. 9: 515–523 (1988).

    CAS  Google Scholar 

  24. R. P. Shugerman, B. I. Hirschowitz, A. S. Brown, R. E. Schrohenloher and J. G. Spenney, A Unique “Mini” Pepsinogen Isolated from Bullfrog Esophageal Glands, J. Biol. Chem. 257: 795–798 (1982).

    PubMed  CAS  Google Scholar 

  25. T. Kageyama and K. Takahashi, Isolation of an Activation Intermediate and Determination of the Amino Acid Sequence of the Activation Segment of Human Pepsinogen A, J. Biochem. 88: 571–582 (1980).

    PubMed  CAS  Google Scholar 

  26. T. Kageyama and K. Takahashi, Monkey Pepsinogens and Pepsins. IV. The Amino Acid Sequence of the Activation Peptide Segment of Japanese Monkey Pepsinogen, J. Biochem. 88: 9–16 (1980).

    PubMed  CAS  Google Scholar 

  27. T. Kageyama and K. Takahashi, Rabbit Pepsinogens. Purification, Characterization, Analysis of the Conversion Process to Pepsin, and Determination of the NH2-Terminal Amino Acid Sequences, Eur. J. Biochem. 141: 261–269 (1984).

    Article  PubMed  CAS  Google Scholar 

  28. T. Kageyama, A. Moriyama and K. Takahashi, Purification and Characterization of Pepsinogens and Pepsins from Asiatic Black Bear, and Amino Acid Sequence Determination of the NH2-Terminal 60 Residues of the Major Pepsinogen, J. Biochem. 94: 1557–1567 (1983).

    PubMed  CAS  Google Scholar 

  29. M. Harboe, P. M. Anderson and B. Foltmann, The Activation of Bovine Pepsinogen — Sequence of the Peptides Released, Identification of a Pepsin Inhibitor, J. Biol. Chem. 249: 4487–4494 (1974).

    PubMed  CAS  Google Scholar 

  30. B. Foltmann and A. L. Jansen, Human Progastricsin — Analysis of Intermediates during Activation into Gastricsin and Determination of the Amino Acid Sequence of the Propart, Eur. J. Biochem. 128: 63–70 (1982).

    Article  PubMed  CAS  Google Scholar 

  31. T. Kageyama and K. Takahashi, Monkey Pepsinogens and Pepsins. VII. Analysis of the Activation Process and Determiantion of the NH2-Terminal 60-Residue Sequence of Japanese Monkey Progastricsin, and Molecular Evolution of Pepsinogens, J. Biochem. 97: 1235–1246 (1985).

    PubMed  CAS  Google Scholar 

  32. B. Foltmann and V B. Pedersen, Comparison of the Primary Structures of Acidic Proteinases and of Their Zymogens, in: “Acid Proteinases” J. Tang, ed., pp. 3–22, Plenum Press, New York (1977).

    Google Scholar 

  33. P. L. Faust, S. Kornfeld and J. M. Chirgwin, Cloning and Sequence Analysis of cDNA for Human Cathepsin D, Proc. Natl. Acad. Sci. U. S. A. 82: 4910–4914 (1985).

    Article  PubMed  CAS  Google Scholar 

  34. A. R. Sielecki, A. A. Fedorov, A. Boodhoo, N. S. Andreeva and M. N. G. James, Molecular and Crystal Structures of Monoclinic Porcine Pepsin Refined at 1·8 Å Reslolution, J. Mol Biol. 214: 143–170 (1990)

    Article  PubMed  CAS  Google Scholar 

  35. W. M. Fitch and E. Margoliash, Construction of Phylogenetic Trees.- A Method Based on Mutation Distances as Estimated from Cytochrome C Sequences is of General Applicability, Science 155: 279–284 (1967).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biophysics and Biochemistry, The University of Tokyo, Tokyo, 113, Japan

    Kenji Takahashi (Faculty of Science), Masao Tanji (Faculty of Science), Etsuko Yakabe (Faculty of Science), Akira Hirasawa (Faculty of Science) & Senerath B. P. Athauda (Faculty of Science)

  2. Department of Cellular and Molecular Biology Primate Research Institute, Kyoto University, Inuyama, Aichi, 484, Japan

    Takashi Kageyama

Authors
  1. Kenji Takahashi
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  2. Masao Tanji
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  3. Etsuko Yakabe
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  4. Akira Hirasawa
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  5. Senerath B. P. Athauda
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  6. Takashi Kageyama
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  1. The University of Tokyo, Tokyo, Japan

    Kenji Takahashi

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Takahashi, K., Tanji, M., Yakabe, E., Hirasawa, A., Athauda, S.B.P., Kageyama, T. (1995). Non-Mammalian Vertebrate Pepsinogens and Pepsins: Isolation and Characterization. In: Takahashi, K. (eds) Aspartic Proteinases. Advances in Experimental Medicine and Biology, vol 362. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1871-6_6

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  • DOI: https://doi.org/10.1007/978-1-4615-1871-6_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5761-2

  • Online ISBN: 978-1-4615-1871-6

  • eBook Packages: Springer Book Archive

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