Isolation and Characterization of Human Gastric Procathepsin E and Cathepsin E

  • Senarath B. P. Athauda
  • Takashi Kageyama
  • Takayuki Takahashi
  • Hideshi Inoue
  • Masao Ichinose
  • Masanori Ukai
  • Kenji Takahashi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 362)

Abstract

Human gastric cathepsin E is an aspartic proteinase present in human gastric mucosa and different from pepsinogens A and C and cathepsin D. It has been localized not only to stomach mucosa, but also to erythrocyte membranes and several lymphoid associated tissues and cells including thymus, spleen, macrophages and polymorphonuclear lymphocytes (1,2). However its physiological function and the process of activation remain obscure. Due to its intracellular localization in lymphoid associated tissues and cells it has been suggested to have a role in immune function (3,4). Studies on cathepsin E has not progressed like those on other aspartic proteinases (pepsinogens and cathepsin D), because of difficulties in obtaining a sufficient amount of the purified native enzyme. Therefore, to understand the structure-function relationship of cathepsin E and to clarify its physiological function, it is important to establish a purification procedure of procathepsin E and cathepsin E for further characterization.

Keywords

Cellulose Hydrolysis High Performance Liquid Chromatography Fluoride Lysin 

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Senarath B. P. Athauda
    • 1
    • 2
  • Takashi Kageyama
    • 3
  • Takayuki Takahashi
    • 1
  • Hideshi Inoue
    • 1
  • Masao Ichinose
    • 1
  • Masanori Ukai
    • 4
  • Kenji Takahashi
    • 1
  1. 1.Department of Biophysics and Biochemistry Faculty of ScienceThe University of TokyoTokyoJapan
  2. 2.Department of Biochemistry Faculty of MedicineUniversity of PeradeniyaPeradeniyaSri Lanka
  3. 3.Department of Cellular and Molecular BiologyPrimate Research InstituteInuyama, AichiJapan
  4. 4.Ukai HospitalNagoyaJapan

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