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Physicochemical Methods in the Study of Biomembranes pp 247–327Cite as

NMR Methods for Measuring Membrane Transport

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Part of the book series: Subcellular Biochemistry ((SCBI,volume 23))

Abstract

The aim of this chapter is to outline the reasons why certain items of information are sought for a description of membrane transport, to describe the various types of NMR experiment that can be used to obtain estimates of the rates of membrane transport, and to illustrate the various NMR procedures with biological examples. The major appeal of the NMR method in this context lies in the fact that the measurements do not usually require the physical separation of the cells or vesicles from their suspending solution. The work described here is a subset of the large number of NMR studies that have been carried out in recent years on living systems (for reviews see Shulman, 1979; Gadian and Radda, 1981; Kuchel, 1981, 1989; Gadian, 1982; Avison et al., 1986; Cerdan and Seelig, 1990; Lundberg et al., 1990). NMR studies of membrane transport have recently been reviewed (Kirk, 1990; Kuchel, 1990; King and Boyd, 1991); the first two reviews are directed mostly at “NMR audiences” and the latter to “biological audiences.” It is the intention that this chapter might be useful to readers who are NMR experts as well as those who are not; the latter may be encouraged to try the NMR counterparts of more conventional experiments and the former may find something of interest in these newer applications of NMR spectroscopy.

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Authors and Affiliations

  1. Department of Biochemistry, University of Sydney, Sydney, NSW, 2006, Australia

    Philip W. Kuchel & Glenn F. King

  2. University Laboratory of Physiology, University of Oxford, OX1 3PT, England

    Kiaran Kirk

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  1. Philip W. Kuchel
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  1. University of Antwerp, Antwerp, Belgium

    Herwig J. Hilderson

  2. University of Sydney, Sydney, Australia

    Gregory B. Ralston

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Kuchel, P.W., Kirk, K., King, G.F. (1994). NMR Methods for Measuring Membrane Transport. In: Hilderson, H.J., Ralston, G.B. (eds) Physicochemical Methods in the Study of Biomembranes. Subcellular Biochemistry, vol 23. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1863-1_7

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