Effect of UV Light on Cytokine Production by Epidermal Cells
The epidermis has been recently recognized as a potent source for cytokines, including interleukins (IL), colony stimulating factors (CSF), tumor necrosis factor (TNF) and growth factors. In addition, ultraviolet B (UVB) light (290–320nm) has turned out to be a strong stimulus for cytokine production by keratinocytes. UVB-irradiation induces the release of IL-1, IL-6, granulocyte macrophage colony stimulating factor (GM-CSF), IL-3 and TNFa, which can be demonstrated at both the protein and the mRNA level. The release of these mediators may be important in the pathogenesis of inflammatory skin reactions following ultraviolet light exposure. In addition, TNFα seems to be involved in the generation of sunburn cells, since intralesional injection of a TNFα antiserum following UV-éxposure results in a significant reduction of sunburn cell formation. Since UV-light generally induces the release of proinflammatory cytokines, the effect of psoralen (8-methoxypsoralen; 8M0P) plus long wavelength (320–400 nm) ultraviolet light (PUVA) on the release of IL-1, IL-6 and TNFα by peripheral blood mononuclear cells (PBMC) was tested. Treatment of PBMC with 8-MOP and UVA resulted in significant down-regulation of the secretion of these mediators by PBMC demonstrated at both the protein and the mRNA level. This effect may contribute to the antinflammatory activity of PUVA.
KeywordsGlutathione Corticosteroid Psoriasis Collagenase Psoralen
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