Effect of UV Light on Cytokine Production by Epidermal Cells
The epidermis has been recently recognized as a potent source for cytokines, including interleukins (IL), colony stimulating factors (CSF), tumor necrosis factor (TNF) and growth factors. In addition, ultraviolet B (UVB) light (290–320nm) has turned out to be a strong stimulus for cytokine production by keratinocytes. UVB-irradiation induces the release of IL-1, IL-6, granulocyte macrophage colony stimulating factor (GM-CSF), IL-3 and TNFa, which can be demonstrated at both the protein and the mRNA level. The release of these mediators may be important in the pathogenesis of inflammatory skin reactions following ultraviolet light exposure. In addition, TNFα seems to be involved in the generation of sunburn cells, since intralesional injection of a TNFα antiserum following UV-éxposure results in a significant reduction of sunburn cell formation. Since UV-light generally induces the release of proinflammatory cytokines, the effect of psoralen (8-methoxypsoralen; 8M0P) plus long wavelength (320–400 nm) ultraviolet light (PUVA) on the release of IL-1, IL-6 and TNFα by peripheral blood mononuclear cells (PBMC) was tested. Treatment of PBMC with 8-MOP and UVA resulted in significant down-regulation of the secretion of these mediators by PBMC demonstrated at both the protein and the mRNA level. This effect may contribute to the antinflammatory activity of PUVA.
KeywordsPeripheral Blood Mononuclear Cell Epidermal Cell Granulocyte Macrophage Colony Stimulate Factor Sunburn Cell Inflammatory Skin Reaction
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- Ansel JC, TA Luger, D Lowry, P Perry, DR Roop and JD Mountz, 1988. The expression and modulation of IL-lα in murine keratinocytes. J. Immunol., 140: 227.Google Scholar
- Bigler CF, DA Norris, WL Weston, and WP Arend, 1991. Interleukin-1 receptor antagonist production by human keratinocytes. Clin. Res., 98: 38.Google Scholar
- Birchall N, C Gamba and T Kupper, 1988. Cutaneous UVB irradiation enhances recovery from bone marrow suppression. Clin. Res., 36: 801A (Abstract).Google Scholar
- Danno K, T Horio and S Imamura, 1991. Infrared radiation suppresses ultraviolet B-induced sunburn cell formation. J. Invest. Dermatol., 96: 1007.Google Scholar
- Luger TA and T Schwarz, 1991. Epidermal cell derived secretory regulins, In: G Schuler, Ed: Epidermal Langerhans Cells, CRC Press, Boca Raton, FL, p. 217.Google Scholar
- Neuner P, A Moller, R Kirnbauer, Ch. Grunwald, A Urbanski, R Knobler, TA Luger and T Schwarz, 1991. Effect of 8-methoxypsoralen (8-MOP) plus UVA on the release of IL-6 by PBMC and epidermal cells. Arch. Derm. Res., 283: 24.Google Scholar
- Sharffetter K, F Wilmroth, M Wlaschek, K Bolsen, P Lehmann, T Krieg, G Goerz, G Plewig and PC Heinrich, 1991. UV-induced autocrine stimulation of fibroblast derived collagenase by IL-6: A possible mechanism in dermal photodamage? Arch. Derm. Res., 283: 58.Google Scholar
- Schreiber S, O Kilgus, E Payuer, R Kutil, A Elbe C Müller and G Stingi, 1992. Cytokine pattern of Langerhans cells isolated from murine epidermal cell cultures. J. Immunol., 149: 3525Google Scholar
- Schwarz T and TA Luger, 1989. Effect of UV irradiation on epidermal cell cytokine production. J. Photochem Photobiol., 84: 1.Google Scholar
- Trefzer U, M Brockhaus, H Loetscher, F Parlow, A Kapp, E Schöpf and J Krutmann, 1991. 55-kd tumor necrosis factor receptor is expressed by human keratinocytes and plays a pivotal role in regulation of human keratinocyte ICAM-1 expression. J. Invest. Dermatol., 96: 543A (Abstract).Google Scholar
- Yoshikawa T and JW Streilein, 1990. TNF-alpha released by UVB-treated keratinocytes impairs induction of contact hypersensitivity. J. Invest. Dermatol., 94: 593.Google Scholar