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Effect of UV Light on Cytokine Production by Epidermal Cells

  • Thomas Schwarz
  • Agatha Urbanski
  • Franz Trautinger
  • Peter Neuner
  • Thomas A. Luger
Part of the NATO ASI Series book series (NSSA, volume 265)

Abstract

The epidermis has been recently recognized as a potent source for cytokines, including interleukins (IL), colony stimulating factors (CSF), tumor necrosis factor (TNF) and growth factors. In addition, ultraviolet B (UVB) light (290–320nm) has turned out to be a strong stimulus for cytokine production by keratinocytes. UVB-irradiation induces the release of IL-1, IL-6, granulocyte macrophage colony stimulating factor (GM-CSF), IL-3 and TNFa, which can be demonstrated at both the protein and the mRNA level. The release of these mediators may be important in the pathogenesis of inflammatory skin reactions following ultraviolet light exposure. In addition, TNFα seems to be involved in the generation of sunburn cells, since intralesional injection of a TNFα antiserum following UV-éxposure results in a significant reduction of sunburn cell formation. Since UV-light generally induces the release of proinflammatory cytokines, the effect of psoralen (8-methoxypsoralen; 8M0P) plus long wavelength (320–400 nm) ultraviolet light (PUVA) on the release of IL-1, IL-6 and TNFα by peripheral blood mononuclear cells (PBMC) was tested. Treatment of PBMC with 8-MOP and UVA resulted in significant down-regulation of the secretion of these mediators by PBMC demonstrated at both the protein and the mRNA level. This effect may contribute to the antinflammatory activity of PUVA.

Keywords

Peripheral Blood Mononuclear Cell Epidermal Cell Granulocyte Macrophage Colony Stimulate Factor Sunburn Cell Inflammatory Skin Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Thomas Schwarz
    • 1
    • 2
  • Agatha Urbanski
    • 3
  • Franz Trautinger
    • 2
  • Peter Neuner
    • 3
  • Thomas A. Luger
    • 2
  1. 1.Department of DermatologyHospital LainzViennaAustria
  2. 2.LBI-DVS Laboratory of Cell Biology Department of DermatologyUniversity of MunsterMunsterGermany
  3. 3.Department of Dermatology IIUniversity of ViennaViennaAustria

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