BCL-2 Gene Rearrangement and Protein Expression in Primary Cutaneous B Cell Lymphomas

  • F. Pezzella
  • M. Santucci
  • A. Neri
  • D. Trecca
  • M. Jones
  • E. Berti
Part of the NATO ASI Series book series (NSSA, volume 265)

Abstract

Primary cutaneous B cell lymphomas (CBCLs) have been recently identified on the basis of their clinical, histological, phenotypic and genotypic features. CBCLs usually show a slow progression with a benign evolution in most cases. Cytological features vary from small lymphocytic to large cell lymphomas. Phenotypic and genotypic data provide evidence for clonal proliferation of mature B lymphocytes. The aim of our study was to analyze the occurrences of bcl-2 gene rearrangement and protein expression in a large series of CBCLs, including many follicular lymphomas. Our results show that only 60% of the CBCLs are positive for bcl-2 protein. Interestingly enough, the small cell lymphomas were all strongly stained, whereas 60% of the large cell lymphomas were negative. Furthermore, only one of 20 follicular CBCLs analyzed showed bcl-2 rearrangement using probes for the major breakpoint region (pFL-1). The pattern of bcl-2 expression observed in primary cutaneous lymphomas, and the absence of bcl-2 gene rearrangement, suggest a similarity between these tumors and MALT B cell lymphomas.

Keywords

Formalin Lymphoma Leukemia Agarose Electrophoresis 

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • F. Pezzella
    • 1
  • M. Santucci
    • 2
  • A. Neri
    • 3
  • D. Trecca
    • 3
  • M. Jones
    • 1
  • E. Berti
    • 4
  1. 1.Department of HematologyJohn Radcliffe HospitalOxfordEngland
  2. 2.Institute of Morbid Anatomy and HistopathologyUniversity of FlorenceFlorenceItaly
  3. 3.Institute of Medical ScienceUniversity of MilanoMilanItaly
  4. 4.First Department of DermatologyUniversity of MilanoMilanItaly

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