BCL-2 Gene Rearrangement and Protein Expression in Primary Cutaneous B Cell Lymphomas
Primary cutaneous B cell lymphomas (CBCLs) have been recently identified on the basis of their clinical, histological, phenotypic and genotypic features. CBCLs usually show a slow progression with a benign evolution in most cases. Cytological features vary from small lymphocytic to large cell lymphomas. Phenotypic and genotypic data provide evidence for clonal proliferation of mature B lymphocytes. The aim of our study was to analyze the occurrences of bcl-2 gene rearrangement and protein expression in a large series of CBCLs, including many follicular lymphomas. Our results show that only 60% of the CBCLs are positive for bcl-2 protein. Interestingly enough, the small cell lymphomas were all strongly stained, whereas 60% of the large cell lymphomas were negative. Furthermore, only one of 20 follicular CBCLs analyzed showed bcl-2 rearrangement using probes for the major breakpoint region (pFL-1). The pattern of bcl-2 expression observed in primary cutaneous lymphomas, and the absence of bcl-2 gene rearrangement, suggest a similarity between these tumors and MALT B cell lymphomas.
KeywordsFormalin Lymphoma Leukemia Agarose Electrophoresis
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