Age-Related Phospholipid Hydroperoxide Levels in Gerbil Brain Measured by HPLC-Chemiluminescence Assay and Their Relation to Hydroxyl Radical Stress — Clinical Implications
Oxidative stress and antioxidant efficiency are implicated in the aging process.1 The formation of PCOOH (phosphatidylcholine hydroperoxide) through oxygen free radical induced lipid peroxidation in cell membrane causes oxidative damage in the brain. In the present study, PCOOH was directly quantified in the hippocampus, cortex and striatum from young (3 months), middle-aged (15 months) and old (20 to 24 months) gerbils by an HPLC-chemiluminescence assay2 (Figure 1). The level of oxidative stress should be differentiated from oxidative damage. For example, an increase in hydroxyl radical or oxidized glutathione levels reflects an increase in oxidative stress, which may not induce oxidative damage. On the other hand, the oxidation of macromolecules such as DNA, protein and lipids (the formation of PCOOH) reflect the actual oxidative damage to the cell.
KeywordsOxidative Stress Oxidative Damage Oxygen Free Radical Chemiluminescence Assay Peroxidative Damage
- 2.J.-R. Zhang, P.K. Andrus, and E.D. Hall, Age-related phospholipid hydroperoxide levels in gerbil brain measured by HPLC-chemiluminescence and their relation to hydroxyl radical stress, Brain Res. (1994) in press.Google Scholar