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Photosensitized Formation of 8-Hydroxy-2’-Deoxyguanosine by A Cationic meso-Substituted Porphyrin

  • Thomas M. Nicotera
  • Robert J. Fiel
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 366)

Abstract

The meso-substituted N-methylpyridyl class of cationic porphyrins are known to have a high affinity for DNA (1). As such, they have been useful tools with which to probe nucleic acid structure and to study a broad range of interactions of cationic ligands with DNA. Activation of this class of porphyrins by visible light produces strand scission in the phosphodiester backbone of DNA at relatively low doses and some metallo-analogs are capable of producing strand scission in the dark. Other substituted porphyrins, hematoporphyrins and methylene blue were found to be less effective under similar conditions. The strand scission activity of cationic porphyrins is not clearly understood, although singlet oxygen has been implicated as a reactive species (1). In this report we have examined the mechanism of photo-induced base damage; specifically, the formation of 8-OHdG. Understanding their role in DNA damage may be useful in screening these compounds as potentially useful agents in photodynamic therapy.

Keywords

Methylene Blue Singlet Oxygen Photodynamic Therapy Roswell Park Cancer Institute Nucleic Acid Structure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Fiel, R.J. J. Biomolec. Struct. & Dynamics, 6:1259 (1989).CrossRefGoogle Scholar
  2. 2.
    Katsuri, C. and Platz, M.S. Photochem. Photobiol. 56:427 (1992).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Thomas M. Nicotera
    • 1
  • Robert J. Fiel
    • 1
  1. 1.Departments of Biophysics and Experimental BiologyRoswell Park Cancer InstituteBuffaloUSA

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