Abstract
CO dehydrogenase has a central role in the anaerobic component of the global carbon cycle. The anaerobic decomposition of complex organic matter involves microbial food chains (consortia) in which acetate is the most abundant intermediate. The acetate is primarily metabolized by acetotrophs, terminal organisms of the food chain, utilizing a pathway in which activated acetate (acetyl-CoA) is cleaved by CO dehydrogenase. The enzyme also catalyzes the synthesis of acetyl-CoA in the acetyl-CoA Ljungdahl-Wood pathway of homoacetogens (see Chapters 1 and 3). Although the physiology of these anaerobes are highly variable, the unity of biochemistry predicts that the underlying chemistry of their metabolism is basically the same. This principal of biochemistry is vividly evident in the CO dehydrogenase (acetyl-CoA cleaving) of acetotrohic methanogens from the Archaea domain; the enzyme has properties that are surprisingly similar to the CO dehydrogenase (acetyl-CoA synthesizing) of homoacetogens from the Bacteria domain. CO dehydrogenases also function in autotrophic methanogens to synthesize acetyl-CoA from CO2 in a process fundamentally similar to the Ljungdahl-Wood pathway. The extreme phylogenetic diversity between the two domains offer a unique opportunity for studies aimed at understanding the mechanism and evolution of CO dehydrogenases in anaerobes.
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References
Abbanat, D. R., and J. G. Ferry. 1990. Synthesis of acetyl-CoA by the carbon monoxide dehydrogenase complex from acetate-grown Methanosarcina thermophila. J. Bacteriol. 172:7145–7150.
Abbanat, D. R., and J. G. Ferry. 1991. Resolution of component proteins in an enzyme complex from Methanosarcina thermophila catalyzing the synthesis or cleavage of acetyl-CoA. Proc. Natl. Acad. Sci. USA 88:3272–3276.
Alber, B. E., and J. G. Ferry. 1994. A novel carbonic anhydrase from the archaeon Methanosarcina thermophila. Proc. Natl. Acad. Sci. USA (in press).
Bhatnagar, L., J. A. Krzycki, and J. G. Zeikus. 1987. Analysis of hydrogen metabolism in Methanosarcina barkeri: regulation of hydrogenase and role of CO-dehydrogenase in H2 production. FEMS Microbiol. Lett. 41:337–343.
Bott, M., B. Eikmanns, and R. K. Thauer. 1986. Coupling of carbon monoxide oxidation to CO2 and H2 with the phosphorylation of ADP in acetate-grown Methanosarcina barkeri. Eur. J. Biochem. 159:393–398.
Bott, M., and R. K. Thauer. 1987. Proton-motive-force-driven formation of CO from CO2 and H2 in methanogenic bacteria. Eur. J. Biochem. 168:407–412.
Bott, M., and R. K. Thauer. 1989. Proton translocation coupled to the oxidation of carbon monoxide to CO2 and H2 in Methanosarcina barkeri. Eur. J. Biochem. 179:469–472.
Clements, A. P., and J. G. Ferry. 1992. Cloning, nucleotide sequence, and transcriptional analyses of the gene encoding a ferredoxin from Methanosarcina thermophila. J. Bacteriol. 174:5244–5250.
Conrad, R., and R. K. Thauer. 1983. Carbon monoxide production by Methanobacterium thermoautotrophicum. FEMS Microbiol. Lett. 20:229–232.
Daniels, L., G. Fuchs, R. K. Thauer, and J. G. Zeikus. 1977. Carbon monoxide oxidation by methanogenic bacteria. J. Bacteriol. 132:118–126.
DeMoll, E., D. A. Grahame, J. M. Harnly, L. Tsai, and T. C. Stadtman. 1987. Purification and properties of carbon monoxide dehydrogenase from Methanococcus vannielii. J. Bacteriol. 169:3916–3920.
DiMarco, A. A., T. A. Bobik, and R. S. Wolfe. 1990. Unusualcoenzymesof methanogenesis. Annu. Rev. Biochem. 59:355–394.
Eggen, R. I. L., A. C. M. Geerling, M. S. M. Jetten, and W. M. Devos. 1991. Cloning, expression, and sequence analysis of the genes for carbon monoxide dehydrogenase of Methanothrix soehngenii. J. Biol. Chem. 266:6883–6887.
Eikmanns, B., and R. K. Thauer. 1984. Catalysis of an isotopic exchange between CO2 and the carboxyl group of acetate by Methanosarcina barkeri grown on acetate. Arch. Microbiol. 138:365–370.
Eikmanns, B., and R. K. Thauer. 1985. Evidence for the involvement and role of a corrinoid enzyme in methane formation from acetate in Methanosarcina barkeri. Arch. Microbiol. 142:175–179.
Eikmanns, B., G. Fuchs, and R. K. Thauer. 1985. Formation of carbon monoxide from CO2 and H2 by Methanobacterium thermoautotrophicum. Eur. J. Biochem. 146:149–154.
Ferry, J. G. 1992a. Biochemistry of methanogenesis. Crit. Rev. Biochem. Mol. Biol. 27:473–503.
Ferry, J. G. 1992b. Methane from acetate. J. Bacteriol. 174:5489–5495.
Fischer, R., and R. K. Thauer. 1989. Methyltetrahydromethanopterin as an intermediate in methanogenesis from acetate in Methanosarcina barkeri. Arch. Microbiol. 151:459–465.
Fischer, R., and R. K. Thauer. 1990a. Methanogenesis from acetate in cell extracts of Methanosarcina barkeri: isotope exchange between CO2 and the carbonyl group of acetyl-CoA, and the role of H2. Arch. Microbiol. 153:156–162.
Fischer, R., and R. K. Thauer. 1990b. Ferredoxin-dependent methane formation from acetate in cell extracts of Methanosarcina barkeri (strain MS). FEBS Lett. 269:368–372.
Fischer, R., P. Gartner, A. Yeliseev, and R. K. Thauer. 1992. N-5-methyltetrahydromethanopterinxoenzyme M methyltransferase in methanogenic archaebacteria is a membrane protein. Arch. Microbiol. 158:208–217.
Gokhale, J. U., H. C. Aldrich, L. Bhatnagar, and J. G. Zeikus. 1993. Localization of carbon monoxide dehydrogenase in acetate-adapted Methanosarcina barkeri. Can. J. Microbiol. 39:223–226.
Grahame, D. A. 1991. Catalysis of acetyl-CoA cleavage and tetrahydrosarcinapterin methylation by a carbon monoxide dehydrogenase-corrinoid enzyme complex. J. Biol. Chem. 266:22227–22233.
Grahame, D. A., and T. C. Stadtman. 1987. Carbon monoxide dehydrogenase from Methanosarcina barkeri. Disaggregation, purification, and physicochemical properties of the enzyme. J. Biol. Chem. 262:3706–3712.
Hammel, K. E., K. L. Cornwell, G. B. Diekert, and R. K. Thauer. 1984. Evidence for a nickel-containing carbon monoxide dehydrogenase in Methanobrevibacter arboriphilicus. J. Bacteriol. 157:975–978.
Harder, S. R., W. P. Lu, B. A. Feinberg, and S. W. Ragsdale. 1989. Spectroelectrochemical studies of the corrinoid iron-sulfur protein involved in acetyl coenzyme-A synthesis by Clostridium thermoaceticum. Biochemistry 28:9080–9087.
Hegeman, G. 1980. Oxidation of carbon monoxide by bacteria. Trends Biochem. Sci. 5:1–3.
Heiden, S., R. Hedderich, E. Setzke, and R. K. Thauer. 1993. Purification of a cytochrome-b containing H2: heterodisulfide oxidoreductase complex from membranes of Methanosarcina barkeri. Eur. J. Biochem. 213:529–535.
Jablonski, P. E., W. P. Lu, S. W. Ragsdale, and J. G. Ferry. 1993. Characterization of the metal centers of the corrinoid/iron-sulfur component of the CO dehydrogenase enzyme complex from Methanosarcina thermophila by EPR spectroscopy and spectroelectrochemistry. J. Biol. Chem. 268:325–329.
Jetten, M. S. M., A. J. M. Stams, and A. J. B. Zehnder. 1989. Purification and characterization of an oxygen-stable carbon monoxide dehydrogenase of Methanothrix soehngenii. FEBS Lett. 181:437–441.
Jetten, M. S. M., W. R. Hagen, A. J. Pierik, A. J. M. Stams, and A. J. B. Zehnder. 1991a. Paramagnetic centers and acetyl-coenzyme A/CO exchange activity of carbon monoxide dehydrogenase from Methanothrix soehngenii. Eur. J. Biochem. 195:385–391.
Jetten, M. S. M., A. J. Pierik, and W. R. Hagen. 1991b. EPR characterization of a high-spin system in carbon monoxide dehydrogenase from Methanothrix soehngenii. Eur. J. Biochem. 202:1291–1297.
Jetten, M. S. M., A. J. M. Stams, and A. J. B. Zehnder. 1992. Methanogenesis from acetate. A comparison of the acetate metabolism in Methanothrix soehngenii and Methanosarcina spp. F EMS Microbiol. Rev. 88:181–198.
Kluyver, A. J., and C. G. T. P. Schnellen. 1947. On the fermentation of carbon monoxide by pure cultures of methane bacteria. Arch. Biochem. 14:57–70.
Köhler, H. P. E. 1988. Isolation of cobamides from Methanothrix soehngenii: 5-methylbenzimidazole as the x-ligand of the predominant cobamide. Arch. Microbiol. 150:219–223.
Krone, U. E., R. K. Thauer, H. P. C. Hogenkamp, and K. Steinbach. 1991. Reductive formation of carbon monoxide from CC14 and FREON-11, FREON-12, and FREON-13 catalyzed by corrinoids. Biochemistry 30:2713–2719.
Krzycki, J. A., and J. G. Zeikus. 1984. Characterization and purification of carbon monoxide dehydrogenase from Methanosarcina barkeri. J. Bacteriol. 158:231–237.
Krzycki, J. A., L. J. Lehman, and J. G. Zeikus. 1985. Acetate catabolism by Methanosarcina barkeri: evidence for involvement of carbon monoxide dehydrogenase, methyl coenzyme M, and methylreductase. J. Bacteriol. 163:1000–1006.
Krzycki, J. A., L. E. Mortenson, and R. C. Prince. 1989. Paramagnetic centers of carbon monoxide dehydrogenase from aceticlastic Methanosarcina barkeri. J. Biol. Chem. 264:7217–7221.
Krzycki, J. A., and R. C. Prince. 1990. EPR observation of carbon monoxide dehydrogenase, methylreductase and corrinoid in intact Methanosarcina barkeri during methanogenesis from acetate. Biochim. Biophys. Acta 1015:53–60.
Kumar, M., and S. W. Ragsdale. 1992. Characterization of the CO binding site of carbon monoxide dehydrogenase from Clostridium thermoaceticum by infrared spectroscopy. J. Am. Chem. Soc. 114:8713–8715.
Laufer, K., B. Eikmanns, U. Frimmer, and R. K. Thauer. 1987. Methanogenesis from acetate by Methanosarcina barkeri: catalysis of acetate formation from methyliodide, CO2, and H2 by the enzyme system involved. Z. Naturforsch. 42c:360–372.
Lu, W. P., S. R. Harder, and S. W. Ragsdale. 1990. Controlled potential enzymology of methyl transfer reactions involved in acetyl-CoA synthesis by CO dehydrogenase and the corrinoid/iron-sulfur protein from Clostridium thermoaceticum. J. Biol. Chem. 265:3124–3133.
Lu, W. P., I. Schiau, J. R. Cunningham, and S. W. Ragsdale. 1993. Sequence and expression of the gene encoding the corrinoid/iron-sulfur protein from Clostridium thermoaceticum and reconstitution of the recombinant protein to full activity. J. Biol. Chem. 268:5605–5614.
Lu, W. P., P. E. Jablonski, M. E. Rasche, J. G. Ferry, and S. W. Ragsdale. 1994. Characterization of the metal centers of the Ni/Fe-S component of the carbon-monoxide dehydrogenase enzyme complex from Methanosarcina thermophila. J. Biol. Chem. 269:9736–9742.
Maupin, J. A., and J. G. Ferry. 1993. Corrinoid-containing cobalt/iron-sulfur component of the CO dehydrogenase complex from Methanosarcina thermophila strain TM-1: cloning, sequencing, and overexpression in Escherichia coli. Abstr. 113, p. 242. 93rd General Meet. Am. Soc. Microbiol. 1993.
Min, H., and S. H. Zinder. 1989. Kinetics of acetate utilization by 2 thermophilic acetotrophic methanogens: Methanosarcina sp strain CALS-1 and Methanothrix sp strain CALS-1. Appl. Environ. Microbiol. 55:488–491.
Mukhopadhyay, B., E. Purwantini, E. C. Demacario, and L. Daniels. 1991. Characterization of a Methanosarcina strain isolated from goat feces, and that grows on H2-CO2 only after adaptation. Curr. Microbiol. 23:165–173.
Nelson, M. J. K., K. C. Terlesky, and J. G. Ferry. 1987. Recent developments on the biochemistry of methanogenesis from acetate. In: Microbial Growth on C-1 Compounds, H. W. van Verseveld and J. A. Duine (eds.), pp. 70–76. Martinus Nijhoff, Dordrecht.
O’Brien, J. M., R. H. Wolkin, T. T. Moench, J. B. Morgan, and J. G. Zeikus. 1984. Association of hydrogen metabolism with unitrophic or mixotrophic growth of Methanosarcina barkeri on carbon monoxide. J. Bacteriol. 158:373–375.
Ohtsubo, S., K. Demizu, S. Kohno, I. Miura, T. Ogawa, and H. Fukuda. 1992. Comparison of acetate utilization among strains of an aceticlastic methanogen, Methanothrix soehngenii Appl. Environ. Microbiol. 58:703–705.
Patel, G. B., and G. D. Sprott. 1990. Methanosaeta concilii Gen-Nov, Sp-Nov (Methanothrix concilii) and Methanosaeta thermoacetophila Nom-Rev, Comb-Nov. Int. J. Syst. Bacteriol. 40:79–82.
Petersen, S. P., and B. K. Ahring. 1991. Acetate oxidation in a thermophilic anaerobic sewage-sludge digestor. The importance of non-aceticlastic methanogenesis from acetate. FEMS Microbiol. Ecol. 86:149–157.
Ragsdale, S. W. 1991. Enzymology of the acetyl-CoA pathway of CO2 fixation. Crit. Rev. Biochem. Mol. Biol. 26:261–300.
Raybuck, S. A., S. E. Ramer, D. R. Abbanat, J. W. Peters, W. H. Orme-Johnson, J. G. Ferry, and C. T. Walsh. 1991. Demonstration of carbon-carbon bond cleavage of acetyl coenzyme A by using isotopic exchange catalyzed by the CO dehydrogenase complex from acetate-grown Methanosarcina thermophila. J. Bacteriol. 173:929–932.
Silveira, R. G., N. Nishio, and S. Nagai. 1991. Growth characteristics and corrinoid production of Methanosarcina barkeri on methanol-acetate medium. J. Ferment. Bioengineer. 71:28–34.
Sowers, K. R., S. F. Baron, and J. G. Ferry. 1984. Methanosarcina acetivorans sp. nov., an acetotrophic methane-producing bacterium isolated from marine sediments. Appl. Environ. Microbiol. 47:971–978.
Stadtman, T. C. 1967. Methane fermentation. Annu. Rev. Microbiol. 21:121–142.
Stupperich, E., and B. Krautler. 1988. Pseudo vitamine B12 or 5-hydroxybenzimidazolyl-cobamide are the corrinoids found in methanogenic bacteria. Arch. Microbiol. 149:268–271.
Terlesky, K. C., M. J. K. Nelson, and J. G. Ferry. 1986. Isolation of an enzyme complex with carbon monoxide dehydrogenase activity containing a corrinoid and nickel from acetate-grown Methanosarcina thermophila. J. Bacteriol. 168:1053–1058.
Terlesky, K. C., M. J. Barber, D. J. Aceti, and J. G. Ferry. 1987. EPR properties of the Ni-Fe-C center in an enzyme complex with carbon monoxide dehydrogenase activity from acetate-grown Methanosarcina thermophila. Evidence that acetyl-CoA is a physiological substrate. J. Biol. Chem. 262:15392–15395.
Terlesky, K. C., and J. G. Ferry. 1988a. Ferredoxin requirement for electron transport from the carbon monoxide dehydrogenase complex to a membrane-bound hydrogenase in acetate-grown Methanosarcina thermophila. J. Biol. Chem. 263:4075–4079.
Terlesky, K. C., and J. G. Ferry. 1988b. Purification and characterization of a ferredoxin from acetate-grown Methanosarcina thermophila. J. Biol. Chem. 263:4080–4082.
Thauer, R. K., K. Jungermann, and K. Decker. 1977. Energy conservation in chemothrophic anaerobic bacteria. Bacteriol. Rev. 41:100–180.
Thauer, R. K., D. Moller-Zinkhan, and A. M. Spormann. 1989. Biochemistry of acetate catabolism in anaerobic chemotrophic bacteria. Annu. Rev. Microbiol. 43:43–67.
Thebrath, B., H. P. Mayer, and R. Conrad. 1992. Bicarbonate-dependent production and methanogenic consumption of acetate in anoxic paddy soil. FEMS Microbiol. Ecol. 86:295–302.
van de Wijingaard, W. M. H., C. van der Drift, and G. D. Vogels. 1988. Involvement of a corrinoid enzyme in methanogenesis from acetate in Methanosarcina barkeri. FEMS Microbiol. Lett. 52:165–172.
Vogels, G. D., J. T. Keltjens, and C. van der Drift. 1988. Biochemistry of methane production. In: Biology of Anaerobic Microorganisms, A. J. B. Zehnder (eds.), pp. 707–770. Wiley, New York.
Westermann, P., B. K. Ahring, and R. A. Mah. 1989a. Temperature compensation in Methanosarcina barkeri by modulation of hydrogen and acetate affinity. Appl. Environ. Microbiol. 55:1262–1266.
Westermann, P., B. K. Ahring, and R. A. Mah. 1989b. Threshold acetate concentrations for acetate catabolism by aceticlastic methanogenic bacteria. Appl. Environ. Microbiol. 55:514–515.
Woese, C. R., O. Kandier, and M. L. Wheelis. 1990. Towards a natural system of organisms. Proposal for the domains archaea, bacteria, and eucarya. Proc. Natl. Acad. Sci. USA 87:4576–4579.
Zinder, S. H., S. C. Cardwell, T. Anguish, M. Lee, and M. Koch. 1984. Methanogenesis in a thermophilic (58°C) anaerobic digestor: Methanothrix sp. as an important aceticlastic methanogen. Appl. Environ. Microbiol. 47:796–807.
Zinder, S. H., and M. Koch. 1984. Non-aceticlastic methanogenesis from acetate: acetate oxidation by a thermophilic syntrophic coculture. Arch. Microbiol. 138:263–272.
Zinder, S. H., K. R. Sowers, and J. G. Ferry. 1985. Methanosarcina thermophila sp. nov., a thermophilic, acetotrophic, methane-producing bacterium. Int. J. Syst. Bacteriol. 35:522–523.
Zinder, S. H., and T. Anguish. 1992. Carbon monoxide, hydrogen, and formate metabolism during methanogenesis from acetate by thermophilic cultures of Methanosarcina and Methanothrix strains. Appl. Environ. Microbiol. 58:3323–3329.
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Ferry, J.G. (1994). CO Dehydrogenase of Methanogens. In: Drake, H.L. (eds) Acetogenesis. Chapman & Hall Microbiology Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1777-1_21
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