Abstract
Acetate is an important CH4 precursor in nature, accounting for two-thirds of the CH4 produced in many natural habitats and in anaerobic bioreactors. Although microbial methanogenesis from acetate was first described in the early 1900s, the mechanism of methanogenesis from acetate was controversial until 1978, when it was demonstrated that a pure culture of Methanosarcina barkeri could grow on acetate (Mah et al., 1978; Smith and Mah, 1978; Weimer and Zeikus, 1978) and convert acetate to CH4 by a decarboxylation mechanism sometimes called the aceticlastic reaction. With the description of a similar mechanism for Methanothrix soehngenii in 1980 (Zehnder et al., 1980), it appeared that acetate decarboxylation was “the” mechanism for methanogenesis from acetate.
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References
Ahring, B. K., P. Westermann, and R. A. Man. 1991. Hydrogen inhibition of acetate metabolism and kinetics of hydrogen consumption by Methanosarcina thermophila TM-1. Arch. Microbiol. 157:38–42.
Balch, W. E., G. E. Fox, M. J. Magrum, C. R. Woese, and R. S. Wolfe. 1979. Methanogens: reevaluation of a unique biological group. Microbiol. Rev. 43:260–296.
Barker, H. A. 1936a. On the biochemistry of the methane fermentation. Arch. Mikrobiol. 6:404–419.
Barker, H.A. 1936b. Studies on the methane-producing bacteria. Arch. Mikrobiol. 7:420–438.
Barker, H. A., S. Ruben, and M. D. Kamen. 1940. The reduction of radioactive carbon dioxide by methane-producing bacteria. Proc. Natl. Acad. Sci. USA 26:426–430.
Barrow, G. M. 1974. Physical Chemistry for the Life Sciences. McGraw Hill Book Co., New York.
Beaty, P. S., N. Q. Wofford, and M. J. Mclnerney. 1987. Separation of Syntrophomonas wolfei from Methanospirillum hungatei using Percoll gradients. Appl. Environ. Microbiol. 53:1183–1185.
Bhatnagar, L., J. A. Krzycki, and J. G. Zeikus. 1987. Analysis of hydrogen metabolism in Methanosarcina barkeri regulation of hydrogenase and role of CO-dehydrogenase in H2 production. FEMS Microbiol. Lett. 41:337–343.
Boone, D. R., and M. P. Bryant. 1980. Propionate-degrading bacterium, Syntrophobacter wolinii sp. nov. gen. nov., from methanogenic ecosystems. Appl. Environ. Microbiol. 40:626–632.
Boone, D. R., R. L. Johnson, and Y. Liu. 1989. Diffusion of the interspecies electron carries H2 and formate in methanogenic ecosystems and its implications in the measurement of K m for H2 or formate uptake. Appl. Environ. Microbiol. 55:1735–1741.
Bryant, M. P., E. A. Wolin, M. J. Wolin, and R. S. Wolfe. 1967. Methanobacillus omelianskii, a symbiotic association of two species of bacteria. Arch. Microbiol. 59:20–31.
Bryant, M. P., L. L. Campbell, C. A. Reddy, and M. R. Crabill. 1977. Growth of Desulfovibrio in lactate or ethanol media low in sulfate in association with H2-utilizing methanogenic bacteria. Appl. Environ. Microbiol. 33:1162–1169.
Bryant, M. P., L. L. Campbell, C. A. Reddy, and M. R. Crabill. 1977. Growth of Desulfovibrio in lactate or ethanol media low in sulfate in association with H2-utilizing methanogenic bacteria. Appl. Environ. Microbiol. 33:1162–1169.
Buswell, A. M., and F. W. Sollo. 1948. The mechanism of the methane fermentation. J. Am. Chem. Soc. 70:1778–1780.
Claypool, G. E., and I. R. Kaplan. 1974. The origin and distribution of methane in marine sediments. In: Natural Gases in Marine Sediments, I. R. Kaplan (ed.), pp. 99–140. Plenum Press, New York.
Conrad, R., and B. Wetter. 1990. Influence of temperature on energetics of hydrogen metabolism in homoacetogenic, methanogenic, and other anaerobic bacteria. Arch. Microbiol. 155:94–98.
Coolhaas, V. C. 1928. Zur kenntnis der dissimilation fettsaurer salze und kohlenhydrate durch thermophile bakerien. Zbl Bakteriol Parasitkenkd Infektionskr Hyg Abt 2 75:161–170.
Cord-Ruwisch, R., H.-J. Steitz, and R. Conrad. 1988. The capacity of hydrogenotrophic anaerobic bacteria to compete for traces of hydrogen depends on the redox potential of the terminal electron acceptor. Arch. Microbiol. 149:350–357.
DiMarco, A. A., T. A. Bobik, and R. S. Wolfe. 1990. Unusual coenzymes of methanogenesis. Ann. Rev. Biochem. 59:355–394.
Eichler, B., and B. Schink. 1985. Fermentation of primary alcohols and diols and pure culture of syntrophically alcohol-oxidizing anaerobes. Arch. Microbiol. 143:60–66.
Ferry, J. D. 1992. Methane from acetate. J. Bacteriol. 174:5489–5495.
Friedrich, M., U. Laderer, and B. Schink. 1991. Fermentative degradation of glycolic acid by defined syntrophic cultures. Arch. Microbiol. 156:398–404.
Grahame, D. A. 1991. Catalysis of acetyl-CoA cleavage and tetrahydrosarcinapterin methylation by a carbon monoxide dehydrogenase-corrinoid enzyme complex. J. Biol. Chem. 266:22227–22233.
Hugenholtz, J., and L. G. Ljungdahl. 1990. Metabolism and energy generation in homoacetogenic clostridia. FEMS Microbiol. Rev. 87:383–390.
King, G. M., M. J. Klug, and D. R. Lovley. 1983. Metabolism of acetate, methanol, and methylated amines in intertidal sediments of Lowes Cove, Maine. Appl. Environ. Microbiol. 45:1848–1853.
Krzycki, J. A., L. J. Lehman, and J. G. Zeikus. 1985. Acetate catabolism by Methanosarcina barkeri: evidence for involvement of carbon monoxide dehydrogenase, methyl coenzyme M and methylreductase. J. Bacteriol. 163:1000–1006.
Länge, S., R. Scholtz, and G. Fuchs. 1989. Oxidative and reductive acetyl CoA/carbon monoxide dehydrogenase pathway in Desulfobacterium autotrophicum. 1. Characterization and metabolic function of the cellular tetrahydropterin. Arch. Microbiol. 151:77–83.
Lee, M. J., and S. H. Zinder. 1988a. Carbon monoxide pathway enzyme activities in a thermophilic anaerobic bacterium grown acetogenically and in a syntrophic acetateoxidizing coculture. Arch. Microbiol. 150:513–518.
Lee, M. J., and S. H. Zinder. 1988b. Hydrogen partial pressures in a thermophilic acetateoxidizing methanogenic coculture. Appl. Environ. Microbiol. 54:1457–1461.
Lee, M. J., and S. H. Zinder. 1988c. Isolation and characterization of a thermophilic bacterium which oxidizes acetate in syntrophic association with a methanogen and which grows acetogenically on H2-CO2. Appl. Environ. Microbiol. 54:124–129.
Ljungdahl, L. G. 1986. The autotrophic pathway of acetate synthesis in acetogenic bacteria. Anna. Rev. Microbiol. 40:415–450.
Ljungdahl, L. G., and H. G. Wood. 1969. Total synthesis of acetate from CO2 by heterotrophic bacteria. Annu. Rev. Microbiol. 23:515–538.
Lovley, D. R. 1985. Minimum threshold for hydrogen metabolism in methanogenic bacteria. Appl. Environ. Microbiol. 49:1530–1531.
Lovley, D. R., and J. G. Ferry. 1985. Production and consumption of H2 during growth of Methanosarcina spp. on acetate. Appl. Environ. Microbiol. 49:247–249.
Man, R. A., M. R. Smith, and L. Baresi. 1978. Studies on an acetate fermenting strain of Methanosarcina. Appl. Environ. Microbiol. 35:1174–1184.
Mclnerney, M. J., M. P. Bryant, and N. Pfennig. 1979. Anaerobic bacterium that degrades fatty acids in association with methanogens. Arch. Microbiol. 122:129–135.
Mclnerney, M. J., M. P. Bryant, R. B. Hespell, and J. W. Costerton. 1981. Syntrophomonas wolfei gen. nov. sp. nov., an anaerobic, syntrophic, fatty acid-oxidizing bacterium. Appl. Environ. Microbiol. 41:1029–1039.
Min, H., and S. H. Zinder. 1989. Kinetics of acetate utilization by two thermophilic acetotrophic methanogens: Methanosarcina sp. strain CALS-1 and Methanothrix sp. strain CALS-1. Appl. Environ. Microbiol. 55:448–491.
Min, H., and S. H. Zinder. 1990. Isolation and characterization of a thermophilic sulfate reducing bacterium. Desulfotomaculum thermoacetoxidans sp. nov. Arch. Microbiol. 153:399–404.
Nölling, J., and W. M. De Vos. 1992. Characterization of the archael plasmid-encoded Type II restriction-modification system Mthti from Methanobacterium thermoformicicum. THF. Homology to the bacterial Ngopii system from Neisseria gonorrhoeae. J. Bacteriol. 174:5719–5726.
Ohtsubo, S., K. Demizu, S. Kohno, I. Miura, T. Ogawa, and H. Fukuda. 1992. Comparison of acetate utilization among strains of an aceticlastic methanogen, Methanothrix soehngenii. Appl. Environ. Microbiol. 58:703–705.
Oremland, R. S. 1988. Biogeochemistry of methanogenic bacteria. In: Biology of Anaerobic Microorganisms, A. J. B. Zehnder (ed.), pp. 641–706. Wiley Interscience, New York.
Petersen, S. P., and B. K. Anting. 1991. Acetate oxidation in a thermophilic anaerobic sewage sludge digestor: the importance of non-acetoclastic methanogenesis from acetate. FEMS Microbiol. Ecol. 86:149–158.
Phelps, T. J., R. Conrad, and J. G. Zeikus. 1985. Sulfate dependent interspecies H2 transfer between Methanosarcina barkeri and Desulfovibrio vulgaris during coculture metabolism of acetate or methanol. Appl. Environ. Microbiol. 50:589–594.
Pine, M. J., and W. Vishniac. 1957. The methane fermentations of acetate and methanol. J. Bacteriol. 73:736–742.
Ragsdale, S. W. 1991. Enzymology of the acetyl-CoA pathway of CO2 fixation. Crit. Rev. Biochem. Mol. Biol. 26:261–300.
Ragsdale, S. W., J. R. Baur, C. M. Gorst, S. R. Harder, W.-P. Lu, D. L. Roberts, J. A. Runquist, and I. Schiau. 1990. The acetyl-CoA synthase from Clostridium thermoaceticum: from gene cluster to active-site metal clusters. FEMS Microbiol. Rev. 87:397–402.
Sansone, F. J., and C. S. Martens. 1981. Methane production from acetate and associated methane fluxes from anoxic coastal sediments. Science 211:707–709.
Schauder, R., B. Eikmanns, R. K. Thauer, F. Widdel, and G. Fuchs. 1986. Acetate oxidation to CO2 in anaerobic bacteria via a novel pathway not involving reactions of the citric acid cycle. Arch. Microbiol. 145:162–172.
Schauder, R., A. Preuss, M. Jetten, and G. Fuchs. 1989. Oxidative and reductive acetyl CoA/carbon monoxide dehydrogenase pathway in Desulfobacterium autotrophicum. 2. Demonstration of the enzymes of the pathway and comparison of CO dehydrogenase. Arch. Microbiol. 151:84–89.
Schönheit, P., J. K. Kristjansson, and R. K. Thauer. 1982. Kinetic mechanism for the ability of sulfate reducers to out-compete methanogens for acetate. Arch. Microbiol. 132:285–288.
Smith, M. R., and R. A. Man. 1978. Growth and methanogenesis by Methanosarcina strain 227 on acetate and methanol. Appl. Environ. Microbiol. 36:870–879.
Sowers, K. R., S. F. Baron, and J. G. Ferry. 1984. Methanosarcina acetivorans sp. nov., an acetotrophic methane-producing bacterium isolated from marine sediments. Appl Environ. Microbiol. 47:971–978.
Sowers, K. R., and R. P. Gunsalus. 1988. Adaptation for growth at various saline concentrations by the archaebacterium Methanosarcina thermophila. J. Bacteriol. 170:998–10
Spormann, A. M., and R. K. Thauer. 1988. Anaerobic acetate oxidation to CO2 by Desulfotomaculum acetoxidans. Demonstration of the enzymes required for the operation of an oxidative acetyl-CoA/carbon monoxide dehydrogenase pathway. Arch. Microbiol. 150:374–380.
Stadtman, T. C., and H. A. Barker. 1949. Studies on the methane fermentation. VII. Tracer experiments on the mechanism of methane formation. Arch. Biochem. 21:256–264.
Stadtman, T. C., and H. A. Barker. 1951. Studies on the methane fermentation IX. The origin of methane in the acetate and methanol fermentation by Methanosarcina. J. Bacteriol. 61:81–86.
Stams, A. J. M., K. C. R. Grolle, C. T. J. J. Fritjers, and J. B. van Lier. 1992. Enrichment of thermophilic propionate-oxidizing bacteria in syntrophy with Methanobacterium thermoautotrophicum or Methanobacterium thermoformicicum. Appl Environ. Microbiol. 58:346–352.
Stupperich, E., and G. Fuchs. 1984. Autotrophic synthesis of activated acetic acid from two CO2 in Methanobacterium thermoautotrophicum. II. Evidence for different origins of acetate carbon atoms. Arch. Microbiol. 139:14–20.
Thauer, R. K., K. Jungermann, and K. Decker. 1977. Energy conservation in chemotrophic anaerobic bacteria. Bacteriol. Rev. 41:100–180.
Thauer, R. K., D. Möller-Zinkhan, and A. M. Spormann. 1989. Biochemistry of acetate catabolism in anaerobic bacteria. Annu. Rev. Microbiol. 43:43–67.
Thiele, J. H., and J. G. Zeikus. 1988. Control of interspecies electron flow during anaerobic digestion: significance of formate transfer versus hydrogen transfer during syntrophic methanogenesis in flocs. Appl. Environ. Microbiol. 54:20–29.
Trouzel, J. P., E. C. de Macario, J. Nölling, W. M. de Vos, T. Zhilina, A. M. Lysenko. 1992. DNA relatedness among some thermophilic members of the genus Methanobacterium. Emendation of the species Methanobacterium thermoautotrophicum and rejection of Methanobacterium thermoformicicum as a synonym of Methanobacterium thermoautotrophicum. Int. J. Syst. Bacteriol. 42:408–411.
Warford, A. L., D. R. Kosiur, and P. R. Doose. 1979. Methane production in Santa Barbara Basin sediments. Geomicrobiol. J. 1:117–137.
Weber, H., K. D. Kulbe, H. Clumiel, and W. Trösch. 1984. Microbial acetate conversion to methane: kinetics, yields and pathways in a two-step digestion process. Appl. Microbiol. Biotechnol. 19:224–228.
Weimer, P. J., and J. G. Zeikus. 1978. Acetate metabolism in Methanosarcina barkeri. Arch. Microbiol. 119:175–182.
Westermann, P., B. K. Ahring, and R. A. Man. 1989. Threshold acetate concentrations for acetate catabolism by acetoclastic methanogenic bacteria. Appl. Environ. Microbiol. 55:514–515.
Widdel, F. 1986. Growth of methanogenic bacteria in pure culture with 2-propanol and other alcohols as hydrogen donors. Appl. Environ. Microbiol. 51:1056–1062.
Widdel, F. 1988. Microbiology and ecology of sulfate-and sulfur-reducing bacteria. In: Biology of Anaerobic Microorganisms, A. J. B. Zehnder (ed.), pp. 469–586. Wiley Interscience, New York.
Winfrey, M. R., and J. G. Zeikus. 1977. Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater lake sediments. Appl. Environ. Microbiol. 33:275–281.
Winter, J. U., and R. S. Wolfe. 1980. Methane formation from fructose by syntrophic associations of Acetobacterium woodii and different strains of methanogens. Arch. Microbiol. 124:73–79.
Witicar, M. J., E. Faber, and M. Schoell. 1986. Biogenic methane formation in marine and freshwater environments. Carbon dioxide reduction vs. acetate fermentation: isotope evidence. Geochem. Cosmochem. Acta 50:693–709.
Wolin, M. J., and T. L. Miller. 1982. Interspecies hydrogen transfer: 15 years later. ASM News 48:561–565.
Zehnder, A. J. B., B. A. Huser, T. D. Brock, and K. Wuhrmann. 1980. Characterization of an acetate-decarboxylating, non-hydrogen-oxidizing methane bacterium. Arch. Microbiol. 124:1–11.
Zehnder, A. J., and W. Stumm. 1988. Geochemistry and biochemistry of anaerobic habitats. In: Biology of Anaerobic Microorganisms, A. J. B. Zehnder (ed.), pp. 1–38, John Wiley and Sons, Inc., New York.
Zeikus, J. G., G. Fuchs, W. Kenealy, and R. K. Thauer. 1977. Oxidoreductases involved in cell carbon synthesis of Methanobacterium thermoautotrophicum. J. Bacteriol. 132:604–613.
Zinder, S. H., and R. A. Man. 1979. Isolation and characterization of a thermophilic strain of Methanosarcina unable to use H2-CO2 for methanogenesis. Appl. Environ. Microbiol. 38:996–1008.
Zinder, S. H., S. C. Cardwell, T. Anguish, M. Lee, and M. Koch. 1984. Methanogenesis in a thermophilic anaerobic digestor: Methanothrix sp. as an important aceticlastic methanogen. Appl. Environ. Microbiol. 47:796–807.
Zinder, S. H., and M. Koch. 1984. Non-aceticlastic methanogenesis from acetate: Acetate oxidation by a thermophilic syntrophic coculture. Arch. Microbiol. 138:263–272.
Zinder, S. H., and A. Elias. 1985. Growth substrate effects on acetate and methanol catabolism in Methanosarcina thermophila strain TM-1. J. Bacteriol. 163:317–323.
Zinder, S. H., K. R. Sowers, and J. G. Ferry. 1985. Methanosarcina thermophila sp. nov., a thermophilic acetotrophic methane producing bacterium. Int. J. Syst. Bacteriol. 35:522–523.
Zinder, S. H., T. Anguish, and T. Lobo. 1987. Isolation and characterization of a thermophilic acetotrophic strain of Methanothrix. Arch. Microbiol. 146:315–322.
Zindel, U., W. Freudenberg, M. Rieth, J. R. Andreesen, J. Schnell, and F. Widdel. 1988. Eubacterium acidaminophilum sp. nov., a versatile amino acid-degrading anaerobe producing or utilizing H2 or formate. Arch. Microbiol. 150:254–266.
Zinder, S. H. 1990. Conversion of acetic acid to methane by thermophiles. FEMS Microbiol. Rev. 75:125–138.
Zinder, S. H., and T. Anguish. 1992. Carbon monoxide, hydrogen, and formate metabolism during methanogenesis from acetate by thermophilic cultures of Methanosarcina and Methanothrix. Appl. Environ. Microbiol. 58:3323–3329.
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Zinder, S.H. (1994). Syntrophic Acetate Oxidation and “Reversible Acetogenesis”. In: Drake, H.L. (eds) Acetogenesis. Chapman & Hall Microbiology Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1777-1_14
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