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A gene located at 72A in Drosophila melanogaster encodes a novel zinc-finger protein that interacts with protein kinase CK2

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Protein Kinase CK2 — From Structure to Regulation

Part of the book series: Molecular and Cellular Biochemistry ((DMCB,volume 35))

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Abstract

Drosophila melanogaster protein kinase CK2 (DmCK2) is a Ser/Thr protein kinase composed of catalytic α and regulatory β subunits associated as an α2β2 heterotetramer. Using the two hybrid system, we have screened a Drosophila embryo cDNA library in order to identify proteins that interact with DmCK2α. One of these cDNAs encodes a novel previously undescribed zinc-finger protein, which we call ZFP47. ZFP47 interacts with DmCK2α but not with DmCK2β, indicating that this interaction is specific for the catalytic subunit of CK2. In situ hybridization to polytene chromosomes indicates that the corresponding gene is located at the 72A interval of chromosome III. Sequence analysis indicates that ZFP47 contains a consensus site for phosphorylation by CK2, 4 C2H2-type zinc-fingers, and a bipartite nuclear localization signal. Consistent with the prediction of a site for phosphorylation by CK2, we demonstrate that ZFP47 is phosphorylated by CK2 purified from Drosophila embryos. These studies demonstrate that ZFP47 is a new physiological partner and substrate of CK2. (Mol Cell Biochem 227: 99–105, 2001)

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Author information

Authors and Affiliations

  1. Department of Biology, USA

    Madhavi Kalive, Regina L. Trott & Ashok P. Bidwai

  2. Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, WV, USA

    Ashok P. Bidwai

Authors
  1. Madhavi Kalive
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  2. Regina L. Trott
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  3. Ashok P. Bidwai
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Editor information

Editors and Affiliations

  1. Cellular and Molecular Biochemistry Research Lab, University of Minnesota, Veterans Affairs Medical Center, One Veterans Drive, Minneapolis, 55417, MN, USA

    Khalil Ahmed

  2. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230, Odense, Denmark

    Olaf-Georg Issinger

  3. Program de Biologia Celular y Molecular, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, 70086, Casilla, Santiago 7, Chile

    Jorge E Allende

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© 2001 Springer Science+Business Media New York

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Kalive, M., Trott, R.L., Bidwai, A.P. (2001). A gene located at 72A in Drosophila melanogaster encodes a novel zinc-finger protein that interacts with protein kinase CK2. In: Ahmed, K., Issinger, OG., Allende, J.E. (eds) Protein Kinase CK2 — From Structure to Regulation. Molecular and Cellular Biochemistry, vol 35. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1723-8_12

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  • DOI: https://doi.org/10.1007/978-1-4615-1723-8_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5696-7

  • Online ISBN: 978-1-4615-1723-8

  • eBook Packages: Springer Book Archive

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