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Visualization and molecular analysis of nuclear import of protein kinase CK2 subunits in living cells

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Protein Kinase CK2 — From Structure to Regulation

Part of the book series: Molecular and Cellular Biochemistry ((DMCB,volume 35))

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Abstract

We have generated fusion proteins between the subunits of CK2 and GFP and characterized their behaviour in living cells. The expressed fusion proteins were functional and interacted with endogenous CK2. Imaging of NIH3T3 cells expressing low level of GFP-CK2α or GFP-CK2β showed that both proteins were mostly nuclear in interphase. Both CK2 subunits contain nuclear localization domains that target them independently to the nucleus. Once in the nucleus, both subunits diffused rapidly in the nucleoplasm. In mitotic cells, CK2 subunits were dispersed throughout the cytoplasm and were not associated to chromatin. Our data are compatible with the idea that each subunit can translocate individually to the nucleus to interact with each other or with important cellular partners. Understanding the molecular mechanisms which regulate the dynamic localization of CK2 subunits will be of central importance. (Mol Cell Biochem 227: 81-90, 2001)

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Author information

Authors and Affiliations

  1. INSERM EMI 0104/BRCE, DBMS, CEA, Grenoble, France

    Véronique Martel, Odile Filhol, Delphine Gerber & Claude Cochet

  2. Almirall Prodesfarma SA, Centro de Investigation, Barcelona, Spain

    Arsenio Nueda

  3. Departamento de Quimica Fisica Aplicada, Universidad Autonoma de Madrid, Madrid, Spain

    Maria Jose Benitez

Authors
  1. Véronique Martel
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  2. Odile Filhol
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  3. Arsenio Nueda
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  4. Delphine Gerber
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  5. Maria Jose Benitez
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  6. Claude Cochet
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Editor information

Editors and Affiliations

  1. Cellular and Molecular Biochemistry Research Lab, University of Minnesota, Veterans Affairs Medical Center, One Veterans Drive, Minneapolis, 55417, MN, USA

    Khalil Ahmed

  2. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230, Odense, Denmark

    Olaf-Georg Issinger

  3. Program de Biologia Celular y Molecular, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, 70086, Casilla, Santiago 7, Chile

    Jorge E Allende

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© 2001 Springer Science+Business Media New York

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Martel, V., Filhol, O., Nueda, A., Gerber, D., Benitez, M.J., Cochet, C. (2001). Visualization and molecular analysis of nuclear import of protein kinase CK2 subunits in living cells. In: Ahmed, K., Issinger, OG., Allende, J.E. (eds) Protein Kinase CK2 — From Structure to Regulation. Molecular and Cellular Biochemistry, vol 35. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1723-8_10

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  • DOI: https://doi.org/10.1007/978-1-4615-1723-8_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5696-7

  • Online ISBN: 978-1-4615-1723-8

  • eBook Packages: Springer Book Archive

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