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Isolated Myocyte Mechanics and Calcium Transients

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Cardiovascular Physiology in the Genetically Engineered Mouse

Part of the book series: Developments in Cardiovascular Medicine ((DICM,volume 238))

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Abstract

Studies of contraction and relaxation of myocardium in isolated heart and cardiac muscle strips can be complicated by coronary circulation (whole heart), chamber geometry (whole heart), extracellular matrix, and neurohumoral factors. Measurement of [Ca2+];transients in intact myocardium preparations can be complicated because fluorescence measurements can be affected by dye loading into cells other than myocytes, motion artifact, inner filter effects, and unstable background fluorescence 1-2. Dissociated cardiac myocytes provide an experimental model that is devoid of the complicating factors mentioned above. In addition, isolated myocytes allow application of single cell voltage-clamp technique to study cardiac ion channels and transporters and of confocal microscopy. The study of isolated myocyte mechanics and [Ca2]; transients has proved to be an especially important tool in the investigation of cardiac physiology and pathophysiology in genetically engineered mice (3-11) and this chapter will focus on the methods that are employed.

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Authors and Affiliations

  1. University of Utah Health Sciences Center, Salt Lake City, UT, 84132, USA

    Zhi Su M.D. Ph.D. & William H. Barry M.D.

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  1. Zhi Su M.D. Ph.D.
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  2. William H. Barry M.D.
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Editors and Affiliations

  1. Case Western Reserve University and University Hospitals of Cleveland, USA

    Brian D. Hoit  & Richard A. Walsh  & 

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Su, Z., Barry, W.H. (2001). Isolated Myocyte Mechanics and Calcium Transients. In: Hoit, B.D., Walsh, R.A. (eds) Cardiovascular Physiology in the Genetically Engineered Mouse. Developments in Cardiovascular Medicine, vol 238. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1653-8_6

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  • DOI: https://doi.org/10.1007/978-1-4615-1653-8_6

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  • Publisher Name: Springer, Boston, MA

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  • Online ISBN: 978-1-4615-1653-8

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