31P NMR Spectroscopy of the Mouse Heart

  • Joanne S. Ingwall
  • Maryam M. Javadpour
  • Wenfeng Miao
Part of the Developments in Cardiovascular Medicine book series (DICM, volume 238)


NMR spectroscopy of tissues and organs yields two kind of information: the steady state tissue content of abundant NMRobservable metabolites and ions and the kinetics of certain enzymes and metabolic pathways. Because these measurements are noninvasive, they can be made repetitively throughout a protocol. They can also be made simultaneously with assessment of physiologic function. In the case for 31P NMR spectroscopy of the isolated heart, and for the heart in situ, it is possible to make measurements of ATP and phosphocreatine (PCr) contents and turnover rates while simultaneously assessing systolic and diastolic performance. Such information is unique and provides a valuable solution to the perennial conflict between biochemistry and physiology. The biochemist dares to extrapolate results obtained from dilute solutions to the protein-rich cytosol, hoping that the results have functional meaning. The physiologist is so convinced of the importance of studying the intact heart, that he/she treats the heart as a black-box about which no molecular information can be known. The biophysicist has the best of both worlds: using NMR spectroscopy, one can learn about biochemical events within the cell while respecting the integrity of the physiological system.


Nuclear Magnetic Resonance Nuclear Magnetic Resonance Spectroscopy Mouse Heart Nuclear Magnetic Resonance Experiment Cardiovascular Physiology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Gadian DG. ed: NMR and its Applications to Living Systems, second edition. 1995; New York, Oxford University Press, Inc.Google Scholar
  2. 2.
    Ingwall JS. Phosphorus nuclear magnetic resonance spectroscopy of cardiac and skeletal muscle. Am J Physiol. 1982; 242:H729–H744.PubMedGoogle Scholar
  3. 3.
    Ingwall JS, Weiss RG. 31P NMR Spectroscopy. The noninvasive tool for the study of the biochemistry of the cardiovascular system. Trends Cardiovasc Med. 1993; 3:29–37.CrossRefGoogle Scholar
  4. 4.
    Moon RB, Richards JH. Determination of intracellular pI-1 by 31P NMR. J Biol Chem. 1973; 248:7276–7278.PubMedGoogle Scholar
  5. 5.
    Gupta RK. On the state of magnesium ion in intact cells as observed by noninvasive P-31 NMR spectroscopy. Int J Quantum Chem. 1980; 7:67–73.Google Scholar
  6. 6.
    Bittl JA, Ingwall JS. Reaction rates of creatine kinase and ATP synthesis in the isolated rat heart. J Biol Chem. 1985; 260(6):3512–3517.PubMedGoogle Scholar
  7. 7.
    Freidrich J, Nascimben L, Liao R, Ingwall JS. Phosphocreatine T1 measurements with and without exchange in the heart. Magn Reson Med. 1993; 30:45–50.CrossRefGoogle Scholar
  8. 8.
    Chu G, Luo W, Slack JP, Tilgmann C, Sweet WE, Spindler M, Saupe KW, Boivin GP, Moravec CS, Matlib MA, Grupp IL, Ingwall JS, Kranias EG. Compensatory mechanisms associated with the hyperdynamic function of phospholamban-deficient mouse hearts. Circ Res. 1996; 78:1064–1076.CrossRefGoogle Scholar
  9. 9.
    Radford NB, Fina M, Benjamin IJ, Moreadith RW, Graves KH, Zhao P, Gavva S, Wiethoff A, Sherry AD, Malloy CR, Williams RS. Cardioprotective effects of 70-kDa heat shock protein in transgenic mice. Proc Natl Acad Sci U S A. 1996; 93(6):2339–42.PubMedCrossRefGoogle Scholar
  10. 10.
    Saupe KW, Spindler M, Tian R, Ingwall JS. Impaired cardiac energetics in mice lacking muscle-specific isoenzymes of creatine kinase. Circ Res. 1998; 82:898–907.PubMedCrossRefGoogle Scholar
  11. 11.
    Van Dorsten FA, Nederhoff MG, Nicolay K, Van Echteld CJ. 31P NMR studies of creatine kinase flux in M-creatine kinase-deficient mouseheart. Am J Physiol. 1998; 275(4 Pt 2):H1191–9.PubMedGoogle Scholar
  12. 12.
    Saupe KW, Spindler M, Hopkins JC, Shen W, Ingwall JS. Kinetic, thermodynamic, and developmental consequences of deleting creatine kinase isoenzymes from the heart: reaction kinetics of the creatine kinase isoenzymes in the intact heart. J Biol Chem. 2000; 275(26):19742–19746.PubMedCrossRefGoogle Scholar
  13. 13.
    Spindler M, Saupe KW, Christe ME, Sweeny HL, Seidman CE, Seidman JG, Ingwall JS. Diastolic dysfunction and altered energetics in the □MHC403/+ mouse model of familial hypertrophic cardiomyopathy. J Clin Invest. 1998; 101:1775–1783.PubMedCrossRefGoogle Scholar
  14. 14.
    Tian R, Miao W, Spindler M, Javadpour MM, McKinney R, Bowman JC, Buttrick PM, Ingwall JS. Long-term expression of protein kinase C in adult mouse hearts improves postischemic recovery. Proc National Acad Sci. 1999; 96(23):13536–13541.CrossRefGoogle Scholar
  15. 15.
    Flogel U, Decking UK, Godecke A, Schrader J. Contribution of NO to ischemia-reperfusion injury in the saline-perfused heart: a study in endothelial NO synthase knockout mice. J Mol Cell Cardiol. 1999; (4):827–36.CrossRefGoogle Scholar
  16. 16.
    Godecke A, Flogel U, Zanger K, Ding Z, Hirchenhain J, Decking UK, Schrader J. Disruption of myoglobin in mice induces multiple compensatory mechanisms. Proc Natl Acad Sci USA. 1999; 96(18):10495–500.PubMedCrossRefGoogle Scholar
  17. 17.
    Cross HR, Steenbergen C, Lefkowitz RJ, Koch WJ, Murphy E. Overexpression of the cardiac beta(2)-adrenergic receptor and expression of a beta-adrenergic receptor kinase 1 (betaARKI) inhibitor both increase myocardial contractility but have differential effects on susceptibility to ischemic injury. Circ Res. 1999; 85(11):1077–84.PubMedCrossRefGoogle Scholar
  18. 18.
    Abel ED, Kaulbach HC, Tian R, Hopkins JCA, Duffy J, Doetschman T, Minnemann T, Boers M-E, Hadro E, Oberste-Berghaus C, Quist W, Lowell BB, Ingwall JS, Kahn BB. Cardiac hypertrophy with preserved contractile function after deletion of GLUT4 from the heart. J Clin Invest. 1999; 104(12):1703–1714.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Joanne S. Ingwall
    • 1
  • Maryam M. Javadpour
    • 1
  • Wenfeng Miao
    • 1
  1. 1.Brigham & Women’s HospitalBostonUSA

Personalised recommendations