Abstract
NMR spectroscopy of tissues and organs yields two kind of information: the steady state tissue content of abundant NMRobservable metabolites and ions and the kinetics of certain enzymes and metabolic pathways. Because these measurements are noninvasive, they can be made repetitively throughout a protocol. They can also be made simultaneously with assessment of physiologic function. In the case for 31P NMR spectroscopy of the isolated heart, and for the heart in situ, it is possible to make measurements of ATP and phosphocreatine (PCr) contents and turnover rates while simultaneously assessing systolic and diastolic performance. Such information is unique and provides a valuable solution to the perennial conflict between biochemistry and physiology. The biochemist dares to extrapolate results obtained from dilute solutions to the protein-rich cytosol, hoping that the results have functional meaning. The physiologist is so convinced of the importance of studying the intact heart, that he/she treats the heart as a black-box about which no molecular information can be known. The biophysicist has the best of both worlds: using NMR spectroscopy, one can learn about biochemical events within the cell while respecting the integrity of the physiological system.
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Ingwall, J.S., Javadpour, M.M., Miao, W. (2001). 31P NMR Spectroscopy of the Mouse Heart. In: Hoit, B.D., Walsh, R.A. (eds) Cardiovascular Physiology in the Genetically Engineered Mouse. Developments in Cardiovascular Medicine, vol 238. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1653-8_10
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DOI: https://doi.org/10.1007/978-1-4615-1653-8_10
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