Abstract
Human milk is a unique reservoir of oligosaccharides. The presence of many of these oligosaccharides is determined genetically and is related to the Lewis blood group and secretor antigen status of each donor. A method to quantitate neutral human milk oligosaccharides was developed. Sample preparation was based on a single centrifugation-filtration step that yields oligosaccharide extracts. These extracts first were fractionated to remove a significant portion of their lactose content and were analyzed using high-pH anion-exchange chromatography. Oligosaccharide profiles from 386 milk samples obtained in this fashion generated quantitative information on lactose, the neutral cores lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNneoT), and the key fucosylated oligosaccharides. Additionally, the profiles provided genetic footprints of the Lewis and secretor status of the donors. Furthermore, unusual profiles that could not have been predicted from known genotypes were found. For this reason, milk glycoproteins were studied using carbohydrate-binding
probes. Results confirm that oligosaccharides are an accurate predictor of the Lewis blood group status of the donor, and that glycosyltransferases have exquisite specificities. The data obtained in this study corroborate that Lewis-related antigens are tissue specific. This attribute of immunodominant carbohydrate sequences has significant implications for epidemiological studies of breast-fed infants.
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References
Blanc B. Biochemical aspects of human milk: comparison with bovine milk. World Rev Nutr Diet 1981; 36:1–89.
Cundell DR, Weiser JN, Shen J, Young A, Tuomanen EI. Relationship between colonial morphology and adherence of Streptococcus pneumoniae. Infect Immun 1995;63:757–761.
Dwek R. Glycobiology: more functions for oligosaccharides. Science 1995;269:1234–1235.
Erney RM, Malone WT, Skelding MB, Marcon AA, Kleman-Leyer KM, O’Ryan ML. Ruiz-Palacios G, Hilty MD, Pickering LK, Prieto PA. Variability of human milk neutral oligosaccharides in a diverse population. J Pediatr Gastroenterol Nutr 2000;30:181–192.
Idanpaan-Heikkila I, Simon PM, Zopf D, Vullo T, Cahill P, Sokol K, Tuomanen E. Oligosaccharides interfere with the establishment and progression of experimental pneumococcal pneumonia. J Infect Dis 1997;176:704–712.
Kobata A. Isolation of oligosaccharides from human milk. In: Ginsburg V, editor. Complex Carbohydrates, Part B. New York: Academic Press; 1972. pp. 262–271.
Kunz C, Rudloff S. Biological functions of oligosaccharides in human milk. Acta Paediatr 1993; 82:903–912.
Prieto PA, Smith DF. A new sialyloligosaccharide from human milk-isolation and characterization using anti-oligosaccharide antibodies. Arch Biochem Biophys 1984;229:650–656.
Stahl B, Steup M, Karas M, Hillenkamp F. Analysis of neutral oligosaccharides by matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem 1991;63:1463–1466.
Thurl S, Muller-Werner B, Sawatzki G. Quantification of individual oligosaccharide compounds from human milk using high-pH anion-exchange chromatography. Anal Biochem 1996;235:202–206.
Zopf D, Ginsburg V. In: Lee EYC, Smith EE. Biology and Chemistry of Eucaryotic Cell Surfaces. Volume 7, Miami Winter Symposia. New York: Academic Press; 1974. pp. 259–271.
Zopf D, Roth S. Oligosaccharide anti-infective agents. Lancet 1996;347:1017–1021.
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Erney, R., Hilty, M., Pickering, L., Ruiz-Palacios, G., Prieto, P. (2001). Human Milk Oligosaccharides. In: Newburg, D.S. (eds) Bioactive Components of Human Milk. Advances in Experimental Medicine and Biology, vol 501. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1371-1_36
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DOI: https://doi.org/10.1007/978-1-4615-1371-1_36
Publisher Name: Springer, Boston, MA
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