Abstract
Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDITOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously. Acidic oligosaccharides could be detected from low-retentiontime GPC fractions with masses up to 4000 Da, which has not been reported before.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Reference
Bahr U, Pfenninger A, Karas M, Stahl B. High-sensitivity analysis of neutral underivatized oligosaccharides by nanoelectrospray mass spectrometry. Anal Chem 1997;69:4530–4535.
Finke B, Stahl B, Pfenninger A, Karas M, Daniel H, Sawatzki G. Analysis of high-molecular weight oligosaccharides from human milk by liquid chromatography and MALDI-MS. Anal Chem 1999;71:375–376.
Papac DI, Wong A, Jones AJ. Analysis of acidic oligosaccharides and glycopeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem 1996;68:3215–3223.
Pfenninger A, Karas M, Finke B, Stahl B, Sawatzki G. MS Investigations of large-size neutral and acidic oligosaccharides from human milk. Proceedings of the 45`hASMS Conference on Mass Spectrometry and Allied Topics; Palm Springs CA; June 1997. p 1020.
Pfenninger A, Karas M, Finke B, Stahl B, Sawatzki G. Matrix optimization for matrix-assisted laser desorption/ionization mass spectrometry of oligosaccharides from human milk. J Mass Spectrom 1999;34:98–104.
Pfenninger A, Karas M, Finke B, Stahl B, Sawatzki G. Structural analysis of underivatized human milk oligosaccharides by NanoESl/Ion Trap MS“. Proceedings of the 46thASMS Conference on Mass Spectrometry and Allied Topics; Orlando FL; June 1998.
Stahl B, Steup M, Karas M, Hillenkamp F Analysis of neutral oligosaccharides by matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem 1991;63:1463–1466.
Stahl B, Thurl S, Zeng J, Karas M, Hillenhamp F, Steup M, Sawatzki G. Oligosaccharides from human milk as revealed by matrix-assisted laser desorption/ionization mass spectrometry. Anal Biochem 1994;223:218–226.
Thurl SW, Offermanns J, Muller-Werner B, Sawatzki G. Determination of neutral oligosaccharide frac-tions from human milk by gel permeation chromatography. J Chromatogr 1991;568:291–300.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media New York
About this chapter
Cite this chapter
Pfenninger, A., Karas, M., Finke, B., Stahl, B., Sawatzki, G. (2001). Mass Spectrometric Investigations of Human Milk Oligosaccharides. In: Newburg, D.S. (eds) Bioactive Components of Human Milk. Advances in Experimental Medicine and Biology, vol 501. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1371-1_35
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1371-1_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5521-2
Online ISBN: 978-1-4615-1371-1
eBook Packages: Springer Book Archive