Vesicular Transport of Soluble Substances into Mouse Milk

  • Jenifer Monks
  • Margaret C. Neville
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 501)


Utilizing a novel protocol to study transport of substances into mouse milk in situ, we have shown that many “fluid-phase” markers are taken up by mammary epithelial cells and deposited in milk. Since the tight junctions are closed and impermeable even to small molecules, extra-alveolar substances (those not synthesized by the alveolar cells) must be transported into the milk by the epithelial cells themselves. The markers we have used include dextran, lucifer yellow dye, horseradish peroxidase, and albumin. Using these markers and immunostaining for endogenous proteins, we have visualized transcytotic vesicles involved in transporting these markers to milk.


Mammary Gland Mammary Epithelial Cell Interstitial Fluid Vesicular Transport Lactate Mammary Gland 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Antohe F, Dobrila L, Heltianu C, Simionescu N, Simionescu M. Albumin-binding proteins function in the receptor-mediated binding and trancytosis of albumin across cultured endothelial cells. Eur J Cell Biol 1993;60:268–275.PubMedGoogle Scholar
  2. Berga S. Electrical potentials and cell-to-cell dye movement in mouse mammary gland during lactation. Am J Physiol 1984;247:C20—C25.PubMedGoogle Scholar
  3. Britton J, Kastin A. Biologically active peptides in milk. Am J Med Sci 1991;301:124–132.PubMedCrossRefGoogle Scholar
  4. Campbell P, Skaar T, Vega J, Baumrucker C. Secretion of insulin-like growth factor-I (IGF-I) and IGF-binding proteins from bovine mammary tissue in vitro. J Endocrinol 1991;128:219–228.PubMedCrossRefGoogle Scholar
  5. Donovan S, OdleJ.Growth factors in milk as mediators of infant development. Annu Rev Nutr 1994;14:147–167.PubMedCrossRefGoogle Scholar
  6. Glimm D, Baracos V, Kennelly J. Northern and in situ hybridisation of analyses of the effects of somatotropin on bovine mammary gene expression. J Dairy Sci 1992;75:2687–2705.PubMedCrossRefGoogle Scholar
  7. Halsey J, Mitchell C, Meyer R, Cebra J. Metabolism of immunoglobulin A in lactating mice: origins of immunoglobulin A in milk. Eur J Immunol 1982;12:107–112.PubMedCrossRefGoogle Scholar
  8. Hendrickson B, Rindisbacher L, Corthesy B, Kendall D, Waltz D, Neutra M, Seidman J. Lack of associ-ation of secretory component with IgA in J chain-deficient mice.JImmunol 1996;157:750–754.PubMedGoogle Scholar
  9. Jensen D, Bessesen D, Etienne J, Eckel R, Neville M. Distribution and source of lipoprotein lipase in mousemammary gland. J Lipid Res 1991;32:733–742.PubMedGoogle Scholar
  10. Jones E. Synthesis and secretion of milk sugars. In: Peaker M, editor. Comparative Aspects of Lactation. London: Academic Press; 1977. pp 77–94.Google Scholar
  11. Koldovsky K. Hormones in milk. Vitam Horm 1995;50:77–149.PubMedCrossRefGoogle Scholar
  12. Lonnerdal B, Forsum E, Hambraeus L. A longitudinal study of the protein, nitrogen, and lactose contents of human milk from Swedish well-nourished mothers. Am JClinNutr 1976;29:1127–1133.PubMedGoogle Scholar
  13. Mostov K, Cardone M. Regulation of protein traffic in polarized epithelial cells. Bioessays 1995;17:129–138.PubMedCrossRefGoogle Scholar
  14. Neville M. The physiological basis of milk secretion. Ann NY Acad Sci 1990;586:1–11.PubMedCrossRefGoogle Scholar
  15. Orlando S. The immunologic significance of breast milk. J Obstet Gynecol Neonatal Nurs 1995;24:678683.Google Scholar
  16. Prosser C, Fleet I. Secretion of insulin-like growth factor-II into milk. Biochem Biophys Res Commun 1992;183:1230–1237. PubMedCrossRefGoogle Scholar
  17. Prosser C. Insulin-like growth factors in milk and mammary gland. J Mammary Gland Biol Neoplasia 1996;1:297–306.PubMedCrossRefGoogle Scholar
  18. Richardson J, Kaushic C, Wira C. Polymeric immunoglobin (Ig) receptor production and IgA transcyto-sis in polarized primary cultures of mature rat uterine epithelial cells. Biol Reprod 1995;53:488–449. PubMedCrossRefGoogle Scholar
  19. Rosato R, Jammes H, Belair L, Puissant C, Kraehenbuhl J-P, Dijian J. Polymeric-Ig receptor gene expres-sion in rabbit mammary gland during pregnancy and lactating: evolution and hormonal regulation.Mol Cell Endocrinol 1995;110:81–87.Google Scholar
  20. Schnitzer J. gp60 is an albumin-binding glycoprotein expressed by continous endothelium involved in albumin transcytosis. Am J Physiol 1992;262:H246—H254.PubMedGoogle Scholar
  21. Shennan D. Mammary gland membrane transport systems. J Mammary Gland Biol Neoplasia 1998; 3:247–258.PubMedCrossRefGoogle Scholar
  22. Telemo E, Hanson L. Antibodies in milk. J Mammary Gland Biol Neoplasia 1996;1:243–250.PubMedCrossRefGoogle Scholar
  23. Tibaduiza E, Bobilya D. Zinc transport across an endothelium includes vesicular cotransport with albumin. J Cell Physiol 1996;167:539–547.PubMedCrossRefGoogle Scholar
  24. Toddywalla V, Kari F, Neville M. Active transport of nitrofurantoin across a mouse mammary epithelial monolayer. J Pharmacol Exp Ther 1997;280:669–676.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Jenifer Monks
    • 1
  • Margaret C. Neville
    • 1
  1. 1.Department of PhysiologyUniversity of Colorado Health Sciences CenterDenver

Personalised recommendations