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Vesicular Transport of Soluble Substances into Mouse Milk

  • Jenifer Monks
  • Margaret C. Neville
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 501)

Abstract

Utilizing a novel protocol to study transport of substances into mouse milk in situ, we have shown that many “fluid-phase” markers are taken up by mammary epithelial cells and deposited in milk. Since the tight junctions are closed and impermeable even to small molecules, extra-alveolar substances (those not synthesized by the alveolar cells) must be transported into the milk by the epithelial cells themselves. The markers we have used include dextran, lucifer yellow dye, horseradish peroxidase, and albumin. Using these markers and immunostaining for endogenous proteins, we have visualized transcytotic vesicles involved in transporting these markers to milk.

Keywords

Mammary Gland Mammary Epithelial Cell Interstitial Fluid Vesicular Transport Lactate Mammary Gland 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Jenifer Monks
    • 1
  • Margaret C. Neville
    • 1
  1. 1.Department of PhysiologyUniversity of Colorado Health Sciences CenterDenver

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