Changes in Lactoferrin and Lysozyme Levels in Human Milk During the First Twelve Weeks of Lactation
Changes in the lactoferrin and lysozyme concentration of human milk during lactation were determined by microparticle-enhanced nephelometric immunoassays of 360 milk samples collected from 64 lactating volunteers. These 360 samples were colostrum from days 1 to 5 postpartum (142 samples), transitional milk from days 6 to 14 (106 samples), and 112 mature milk samples obtained from days 15 to 28 (34 samples), from days 29 to 56 (50 samples) and from days 57 to 84 postpartum (28 samples). The concentration and percentage of lactoferrin vs. total protein were found to be significantly higher in colostrum (5.8 g/L, 27%) than in transitional milk (3.1 g/L, 22%) or day 15 to 28 mature milk (2 0 g/L, 19%), then increased in day 29 to 56 mature milk (2 2 g/L, 22%) and day 57 to 84 mature milk (3 3 g/L, 30%). The concentration of lysozyme decreased from colostrum (0.37 g/L) to transitional milk (0.27 g/L) and day 15 to 28 mature milk (0.24 g/L), then increased in day 29 to 56 mature milk (0 33 g/L) and was highest in day 57 to 84 mature milk (0 89 g/L). The percentage of lysozyme vs. total protein was found to be always rising during lactation: colostrum, 2%; transitional milk, 2%; days 15 to 28, 2%; days 29 to 56, 3%; and days 57 to 84 mature milk, 8%.
KeywordsMilk Sample Human Milk Fast Protein Liquid Chromatography Electron Spin Echo Envelope Modulation Mature Milk
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- Jolies J, Jolies P. Human tear and human milk lysozyme. Biochemistry 1967;6:411–417.Google Scholar
- Kawakami H, Lönnerdal B. Isolation and function of a receptor for human lactoferrin in human fetal intestinal brush-border membranes. Am J Physiol 1991;261:841–846.Google Scholar
- Montagne P, Laroche P, Bessou T, Cuillière ML, Varcin P, Duheille J. Measurement of eleven serum proteins by microparticle-enhanced nephelometric immunoassay. J Clin Chem Clin Biochem 1992a; 30:217–222.Google Scholar
- Montagne P, Cuillière ML, Marchai E, El Bari N, Montagne M, Benali M, Faure G, Duheille J, Humbert G, Linden G, Heurtaux N, Blesche JL, Gosselin D, Desmares A, Delahaye D. Application of microparticle-enhanced nephelometric immunoassay of alpha-, beta-and kappa-caseins to evaluate quality of milk, from the production to the valorization in cheese industry. Lait 1995;75:221–237.CrossRefGoogle Scholar
- Stuart J, Norrel S, Harrington JP. Kinetic effect of human lactoferrin on the growth of Escherichia coli. J Biochem 1984;16:1043–1047.Google Scholar