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Growth Rates of a Human Colon Adenocarcinoma Cell Line are Regulated by the Milk Protein Alpha-Lactalbumin

  • Lisa G. Sternhagen
  • Jonathan C. Allen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 501)

Abstract

The whey protein a-lactalbumin, derived from human milk, has been shown to inhibit proliferation of mammary epithelial cells and rat kidney cells. We have shown that bovine a-lactalbumin also has antiproliferative effects in human colon adenocarcinoma cell lines. During a 5-day dose-dependent growth study, bovine a-lactalbumin was added to Caco-2 or HT-29 monolayers in amounts from 5 to 35 µg/mL. Low concentrations of a-lactalbumin (10-25 µg/mL) stimulated growth during the first 3 to 4 days. After growing for 4 days, proliferation ceased and viable cell numbers decreased dramatically in the a-lactalbumin-treated cultures, suggesting a delayed initiation of apoptosis. This experiment demonstrates the acute bioactive effects of small concentrations of a-lactalbumin, compared with the high concentrations of other proteins in the media. These results suggest that a-lactalbumin in milk may promote health by inhibiting growth of potential cancer cells. Further studies will identify the role of calcium in the bioactivity of a-lactalbumin

Keywords

Human Milk Whey Protein Human Colon Adenocarcinoma Cell Line Bovine Whey Protein Cell Replication Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Lisa G. Sternhagen
    • 1
  • Jonathan C. Allen
    • 1
  1. 1.Interdepartmental Nutrition Program Department of Food ScienceNorth Carolina State UniversityRaleigh

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