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Analytical Assays to Characterise Adenoviral Vectors and Their Applications

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Manufacturing of Gene Therapeutics

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Abstract

Recombinant adenovirus preparations are used for gene delivery in a number of clinical gene therapy approaches. Adenovirus offers short term (transient) gene expression without integration into the host cell genome1. The adenovirus is a complex biological system containing several different structural proteins and a linear double stranded DNA molecule held together by non-covalent interactions2. The classical assays used to characterise the adenovirus preparations include infectivity analysis (typically a plaque assay format3), SDS-PAGE analysis of the adenoviral proteins2,4,5 and the estimation of virus concentration by lysing the virus in SDS and measuring absorption at 260 nm in the lysate2.

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Authors and Affiliations

  1. Berlex Biosciences, 15049 San Pablo Avenue, Richmond, CA, 94804-4089, USA

    Elisabeth Lehmberg, Michael T. McCaman, Joseph A. Traina, Peter K. Murakami, James G. Files, Bruce Mann, Linh Do, Mei P. Tan, Spencer Tse, Tao Yu, Jeffrey W. Nelson, Juan Irwin, John Irving, Eirik Nestaas & Erno Pungor Jr.

Authors
  1. Elisabeth Lehmberg
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  2. Michael T. McCaman
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  3. Joseph A. Traina
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  4. Peter K. Murakami
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  5. James G. Files
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  6. Bruce Mann
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  7. Linh Do
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  8. Mei P. Tan
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  9. Spencer Tse
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  10. Tao Yu
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  11. Jeffrey W. Nelson
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  12. Juan Irwin
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  13. John Irving
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  14. Eirik Nestaas
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  15. Erno Pungor Jr.
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Editors and Affiliations

  1. Littlebourne, Kent, England

    G. Subramanian

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© 2002 Springer Science+Business Media New York

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Lehmberg, E. et al. (2002). Analytical Assays to Characterise Adenoviral Vectors and Their Applications. In: Subramanian, G. (eds) Manufacturing of Gene Therapeutics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1353-7_10

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  • DOI: https://doi.org/10.1007/978-1-4615-1353-7_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5512-0

  • Online ISBN: 978-1-4615-1353-7

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