Partitioning: Experimental Procedures and Examples
When a compound is placed in a system composed of two immiscible solvents, the distribution of the compound was shown in Chapter 5, Eq. (5.4), to be directly related to the difference between the standard state free energies for the compound in the two solvents. Since the standard state free energies are constant for a specific system, this difference will also be constant and a partition coefficient is therefore an equilibrium constant. As long as the system remains constant, that is, the temperature and components do not change, the partition coefficient for a specific species will not change. Under these conditions the partition profile can be determined with, for instance, a change in pH. In this chapter the results of partitioning studies using several techniques will be discussed.
KeywordsHydrolysis HPLC COrn Hydroxide Syringe
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