Identification of Specific Variations within the HE, S1, and ORF4 Genes of Bovine Coronaviruses Associated with Enteric and Respiratory Diseases in Dairy Cattle

  • Anne-Marie Gélinas
  • Am-J Sasseville
  • Serge Dea
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

The bovine Coronavirus (BCV) is the causative agent of enteric diseases including neonatal calf diarrhea (NCD) (Dea et al., 1980; Mebus et al., 1973), winter dysentery (WD) (Benfield and Saif, 1990; Dea et al., 1995), and chronic shedding in adult cattle (AD) (Tsunemitsu et Saif, 1995). BCV can also infect the upper respiratory tract of growing calves causing pneumonia (Chouljenko et al., 1998; Reynolds et al., 1985). The respiratory BCV (RBCV) is now recognized as an important agent associated to shipping fever (Storz et al., 1996). The virion possesses a single stranded, non segmented RNA genome of positive polarity, and is made of 5 structural proteins: the nucleocapsid phosphoprotein (N: 52 kDa), the matrix glycoprotein (M: 25 kDa), the peplomer glycoprotein (S: 200 kDa), the small membrane protein (E: 9.5 kDa) and the hemagglutinin esterase glycoprotein (HE: 140 kDa) (Spaan et al., 1988). Both S and HE are able to agglutinate red blood cells and elicit production of neutralizing antibodies (Dea et Tijssen, 1989; Deregt and Babiuk, 1987; Vautherot et al, 1992). The S protein has an important role to play in tropism being responsible for binding to the host cell (Spaan et al., 1988). The regions situated between the S and M genes (ORF4 and ORF5) may also be implicated in the tropism of coronaviruses (Mounir et al., 1992).

Keywords

Attenuation Adenocarcinoma Shipping Pneumonia Glycine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Benfield DA, Saif LJ (1990) Cell culture propagation of a Coronavirus isolated from cows with winter dysentery. J Clin Microbiol 28: 1454–1457PubMedGoogle Scholar
  2. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Ann Biochem 162: 156–159CrossRefGoogle Scholar
  3. Chouljenko VN, Kousoulas KG, Lin X, Storz, J (1998) Nucleotide and predicted amino acid sequences of all genes encoded by the 3’ genomic portion (9.5 kb) of respiratory bovine coronaviruses and comparisons among respiratory and enteric coronaviruses. Virus genes 17:33–42PubMedCrossRefGoogle Scholar
  4. Dea S, Roy RS, Begin, ME (1980) Bovine Coronavirus isolation in cell cultures. Am J Vet Res 41, 30–38.PubMedGoogle Scholar
  5. Dea S, Michaud L, Milane G (1995) Comparison of bovine Coronavirus isolates associated with neonatal calf diarrhoea and winter dysentery in adult dairy cattle in Québec. J Gen Virol 76, 1263–1270PubMedCrossRefGoogle Scholar
  6. Dea S, Tijssen P (1989) Antigenic and Polypeptide structure of turkey enteric coronaviruses as defined by monoclonal antibodies. J Gen Virol 70:, 1725–1741PubMedCrossRefGoogle Scholar
  7. Deregt D, Babiuk LA (1987) Monoclonal antibodies to bovine Coronavirus: characteristics and topographical mapping of neutralizing epitopes on the E2 and E3 glycoproteins. Virology 161, 410–420PubMedCrossRefGoogle Scholar
  8. Mebus CA, Stair EL, Rhodes MB, Twiehaus MJ (1973) Neonatal calf diarrhea: propagation, attenuation, and characteristics of a coronavirus-like agent. Am J Vet Res 34, 145–150PubMedGoogle Scholar
  9. Milane G, Kourtesis AB, Dea S (1997) Characterization of monoclonal antibodies to the hemagglutinin-esterase glycoprotein of a bovine Coronavirus associated with winter dysentery and cross-reactivity to field isolates. J Clin Microbiol 35:33–40.PubMedGoogle Scholar
  10. Mounir S., and Talbot, P. J. (1993) Human Coronavirus OC43 RNA lacks two open reading framed located downstream of the S gene of bovine Coronavirus. Virology 192, 355–360.PubMedCrossRefGoogle Scholar
  11. Parker MD, Yoo D, Babiuk LA (1990) Expression and secretion of the bovine Coronavirus hemagglutinin-esterase glycoprotein by insect cells infected with recombinant baculoviruses. J Virol 64, 1625–1629PubMedGoogle Scholar
  12. Reynolds DJ, Debney T J, Hall GA, Thomas LH, Parsons KR (1985) Studies on the relationship between coronaviruses from the intestinal and respiratory tracts of calves. Arch Virol 85, 71–83PubMedCrossRefGoogle Scholar
  13. Spaan WD, Cavanagh D, Horzinek MC (1988) Coronaviruses: structure and genome expression. J Gen Virol 69, 2939–2952PubMedCrossRefGoogle Scholar
  14. Storz J, Stine L, Liem A, et al. (1996) Coronavirus isolation from nasal swabs samples in cattle with signs of respiratory tract disease after shipping. JAVMA 208, 1452–1454PubMedGoogle Scholar
  15. Tsunemitsu H, Saif LJ (1995) Antigenic and biological comparisons of bovine coronaviruses derived from neonatal calf diarrhea and winter dysentery of adult cattle. Arch Virol 140, 1303–1311PubMedCrossRefGoogle Scholar
  16. Vautherot JF, Madelaine MF, Boireau P, Laporte J (1992). Bovine Coronavirus peplomer glycoproteins: detailed antigenic analysis of SI, S2 and HE. J Gen Virol 73, 1725–1737.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Anne-Marie Gélinas
    • 1
  • Am-J Sasseville
    • 1
  • Serge Dea
    • 1
  1. 1.Centre de Recherche en Microbiologie et BiotechnologieINRS-Institut Armand-Frappier, Université du QuébecLavalCanada

Personalised recommendations