Abstract
Coronavirus is a large enveloped virus containing a 27-32 kb-long single-stranded, positive-sense RNA genome (Lee et al 1991). Coronaviruses generally have three envelope proteins S, M, and E. N protein and the viral genomic RNA form a helical nucleocapsid (Macnaughton et al 1978).
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Keywords
- Northern Blot Analysis
- Salt Buffer
- Mouse Hepatitis Virus
- Viral Envelope Protein
- Sucrose Gradient Centrifugation
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References
Baric, R. S., Fu K., Schaad, M. C., and Stohlman, S. A., 1990, Establishing a genetic recombination map for murine Coronavirus strain A59 complementation groups. Virology 177:646–656.
Fosmire, J. A., Hwang K., and Makino, S., 1992, Identification and characterisation of a Coronavirus packaging signal. J. Virol. 66:3522–3530.
Hirano, N., Fujiwara K., Hino, S., and Matsumoto, M., 1974, Replication and plaque formation of mouse hepatitis virus (MHV-2) in mouse cell line DBT culture. Arch. Gesamte. Virusforch. 44:298–302.
Kim, K. H., Narayanan, K., and Makino, S., 1997, Assembled Coronavirus from complementation of two defective interfering RNAs. J. Virol. 71:3922–3931.
Lee, H.-J., Shieh, C.-K.,. Gorbalenya, A. E., Eugene, E. V., La Monica, N., Tuler, J., Bagdzhadzhyan, A., and Lai, M. M. C., 1991, The complete sequence (22 kilobases) of murine Coronavirus gene 1 encoding the putative proteases and RNA Polymerase. Virology 180:567–582.
Macnaughton, M. R., Davies, H. A., and Nermut, M. V., 1978, Ribonucleoprotein-like structures from Coronavirus particles. J. Gen. Virol. 39:545–549.
Makino, S., Taguchi, F., Hayami, M., and Fujiwara, K., 1983, Characterisation of small plaque mutants of mouse hepatitis virus, JHM strain. Microbiol. Immunol. 27:445–454.
Makino, S., Joo, M., and Makino, J. K., 1991, A system for study of Coronavirus mRNA synthesis: a regulated, expressed subgenomic defective interfering RNA results from intergenic site insertion. J. Virol. 65:6031–6041.
Narayanan, K., Maeda, A., Maeda J., and Makino, S., Characterisation of the Coronavirus M protein and nucleocapsid interaction in infected cells. In press.
Sturman, L. S., Holmes, K. V., and Behnke, J., 1980, Isolation of Coronavirus envelope glycoproteins and interaction with the viral nucleocapsid. J. Virol. 33:449–462.
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© 2001 Springer Science+Business Media New York
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Narayanan, K., Makino, S. (2001). Characterization of Nucleocapsid-M Protein Interaction in Murine Coronavirus. In: Lavi, E., Weiss, S.R., Hingley, S.T. (eds) The Nidoviruses. Advances in Experimental Medicine and Biology, vol 494. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1325-4_85
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DOI: https://doi.org/10.1007/978-1-4615-1325-4_85
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