Spread of Hemagglutinating Encephalomyelitis Virus (HEV) in the CNS of Rats Inoculated by Intranasal Route
Part of the
Advances in Experimental Medicine and Biology
book series (AEMB, volume 494)
HEV is known to cause vomiting and wasting disease or encephalomyelitis in piglets (Andries et al., 1980,1981; Mengeling et al., 1972; Roe et al., 1958). In experimental oronasal infection of piglets, the virus spread to the CNS predominantly via the nerve pathways (Andries et al., 1980, 1981). In our previous study, 4-week-old rats died of encephalitis after inoculation by different routes including intranasal route (Hirano et al., 1993). In the infected animals, the virus reaches the CNS through the nerve pathways from peripheral nerve. Our previous studies have demonstrated that HEV propagated through nervous route and its infection was restricted to neurons after inoculation into sciatic nerve or footpad of rats (Hirano et al., 1995, 1998). Our findings suggest that HEV is useful as a trans-synaptic tracer for analyzing neuronal connections in the CNS.
KeywordsDentate Gyrus Superior Colliculus Cerebellar Cortex Inferior Colliculus Positive Neuron
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Andries, K., and Pensaert, M. B. 1980. Immunofluorescence studies on the pathogenesis of hemagglutinating encephalomyelitis virus infection in pigs after oronasal inoculation. Am. J. Vet. Res.
Andries, K., and Pensaert, M. B. 1981. Vomiting and wasting disease; a Coronavirus infection of pigs. Adv. Exp. Med. Biol.
Hirano, N., Haga, S., and Fujiwara, K. 1993. The route of transmission of hemagglutinating encephalomyelitis virus (HEV) 67N strain in 4-week-old rats. Adv. Exp. Med. Biol.
Hirano, N., Nomura, R., Tawara, K., Ono, K., and Iwasaki, Y. 1995. Neuronal spread of hemagglutinating encephalomyelitis virus (HEV) 67N strain in 4-week-old rats. Adv. Exp. Med Biol
Hirano, N., Ono, K., Takasawa, H., and Haga, S. 1990. Replicaiton and plaque formation of swine hemagglutinating encephalomyelitis virus (67N) in swine cell line, SK-K culture. J. Virol. Methods
Hirano, N., Tohyama, K., and Taira, H. 1998. Spread of hemagglutinating encephalomyelitis virus from peripheral nerve to the CNS. Adv. Exp. Med. Biol.
Lavi, E., Fishman, P., Highkin, M., and Weiss, S. 1988. Limbic encephalitis after inhalation of a murine Coronavirus. Lab. Invest.
Mengeling, W. L., Boothe, A. D., and Richite, A. E. 1972. Characterization of a Coronavirus (Strain 67N) of pigs. Am. J. Vet. Res.
Mengeling, W. L., and Cutlip, R. C. 1976. Pathogenicity of field isolates of hemagglutinating encephalomyelitis virus for neonatal pigs. J. Am. Vet. Assoc.
Roe, C. K., and Alexander, T. J. L. 1958. A disease of nursing pigs previously unreported in Ontario. Canad. Comp. Med.
© Springer Science+Business Media New York 2001