Genetic Variation of ORFs 3 and 4 of Equine Arteritis Virus
The open reading frames (ORFs) 3 and 4 of equine arteritis virus (EAV) encode the GP3 and GP4 proteins, respectively. The GP3 protein of EAV is an extensively glycosylated membrane protein that is likely anchored by the uncleaved signal sequence (Hedges et al., 1999a). ORF 4 is predicted to encode a membrane glycoprotein. The goal of this study was to determine the variation in ORFs 3 and 4 and their encoded GP3 and GP4 proteins amongst a large number of EAV strains, including those amplified directly from the semen of carrier stallions.
KeywordsGlycosylation Site Field Isolate Strong Selective Pressure Equine Arteritis Virus Infectious cDNA Clone
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- Balasuriya, U. B. R., Patton, J. F., Rossitto, P. V., Timoney, P. J., McCollum, W. H., and MacLachlan, N. J. (1997). Neutralization determinants of laboratory strains and field isolates of equine arteritis virus: Identification of four neutralization sites in the amino-terminal ectodomain of the GL envelope glycoprotein. Virology 232, 114–128.PubMedCrossRefGoogle Scholar
- Mardassi, H., Gonin, P., Gagnon, C. A., Massie, B., and Dea, S. (1998). A subset of porcine reproductive and respiratory syndrome virus GP3 glycoprotein is released into the culture medium of cells as a non-virion-associated and membrane-free (soluble) form. J. Virol. 72, 6298–6306.PubMedGoogle Scholar
- Patton, J. F., Balasuriya, U. B. R., Hedges, J. F., Schweidler, T. M., Hullinger, P. J., and MacLachlan, N. J. (1998). Phylogenetic characterization of a highly attenuated strain of equine arteritis virus from the semen of a persistently infected standardbred stallion. Arch. Virol. 144, 817–827.CrossRefGoogle Scholar
- van Nieuwstadt, A. P., Meulenberg, J. J. M., van Essen-Zandbergen, A., Peterson-den Besten, A., Bende, R. J., Moormann, R. J., and Wensvoort, G. (1996). Proteins encoded by open reading frames 3 and 4 of the genome of lelystad virus (Arteriviridae) are structural proteins of the virion. J. Virol. 70, 4746–4772.Google Scholar