Genetic Variation of ORFs 3 and 4 of Equine Arteritis Virus

  • Jodi Hedges
  • Udeni B. R. Balasuriya
  • Jb Topol
  • Dustin W. Lee
  • N. James Maclachlan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

The open reading frames (ORFs) 3 and 4 of equine arteritis virus (EAV) encode the GP3 and GP4 proteins, respectively. The GP3 protein of EAV is an extensively glycosylated membrane protein that is likely anchored by the uncleaved signal sequence (Hedges et al., 1999a). ORF 4 is predicted to encode a membrane glycoprotein. The goal of this study was to determine the variation in ORFs 3 and 4 and their encoded GP3 and GP4 proteins amongst a large number of EAV strains, including those amplified directly from the semen of carrier stallions.

Keywords

Cysteine Serine Tryptophan Threonine Asparagine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Archambault, D., Laganiere, G., Carman, S., and St-Laurent, G. (1997). Comparison of nucleic and amino acid sequences and phylogenetic analysis of open reading frames 3 and 4 of various equine arteritis virus isolates. Vet. Res. 28, 505–516.PubMedGoogle Scholar
  2. Balasuriya, U. B. R., Hedges, J. F., Timoney, P. J., McCollum, W. H., and MacLachlan, N. J. (1999). Genetic stability of equine arteritis virus during horizontal and vertical transmission in an outbreak of equine viral arteritis. J. Gen. Virol. 80, 1949–1958.PubMedGoogle Scholar
  3. Balasuriya, U. B. R., Patton, J. F., Rossitto, P. V., Timoney, P. J., McCollum, W. H., and MacLachlan, N. J. (1997). Neutralization determinants of laboratory strains and field isolates of equine arteritis virus: Identification of four neutralization sites in the amino-terminal ectodomain of the GL envelope glycoprotein. Virology 232, 114–128.PubMedCrossRefGoogle Scholar
  4. Hedges, J. F., Balasuriya, U. B. R., Timoney, P. J., McCollum, W. H., and MacLachlan, N. J. (1996). Genetic variation in open reading frame 2 of field isolates and laboratory strains of equine arteritis virus. Virus Res. 42, 41–52.PubMedCrossRefGoogle Scholar
  5. Hedges, J. F., Balasuriya, U. B. R., and MacLachlan, N. J. (1999a). The open reading frame 3 of equine arteritis virus encodes an immunogenic glycosylated, integral membrane protein. Virology 264, 92–98.PubMedCrossRefGoogle Scholar
  6. Hedges, J. F., Balasuriya, U. B. R., Timoney, P. J., McCollum, W. H., and MacLachlan, N. J. (1999b). Genetic divergence with emergence of phenotypic variants of equine arteritis virus during persistent infection of stallions. J. Virol. 73, 3672–3681.PubMedGoogle Scholar
  7. Mardassi, H., Gonin, P., Gagnon, C. A., Massie, B., and Dea, S. (1998). A subset of porcine reproductive and respiratory syndrome virus GP3 glycoprotein is released into the culture medium of cells as a non-virion-associated and membrane-free (soluble) form. J. Virol. 72, 6298–6306.PubMedGoogle Scholar
  8. Patton, J. F., Balasuriya, U. B. R., Hedges, J. F., Schweidler, T. M., Hullinger, P. J., and MacLachlan, N. J. (1998). Phylogenetic characterization of a highly attenuated strain of equine arteritis virus from the semen of a persistently infected standardbred stallion. Arch. Virol. 144, 817–827.CrossRefGoogle Scholar
  9. van Nieuwstadt, A. P., Meulenberg, J. J. M., van Essen-Zandbergen, A., Peterson-den Besten, A., Bende, R. J., Moormann, R. J., and Wensvoort, G. (1996). Proteins encoded by open reading frames 3 and 4 of the genome of lelystad virus (Arteriviridae) are structural proteins of the virion. J. Virol. 70, 4746–4772.Google Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Jodi Hedges
    • 1
  • Udeni B. R. Balasuriya
    • 1
  • Jb Topol
    • 1
  • Dustin W. Lee
    • 2
  • N. James Maclachlan
    • 1
  1. 1.The Bernard and Gloria Salick Equine Viral Diseases Laboratory, Department of Pathology, Microbiology and ImmunologySchool of Veterinary MedicineDavisUSA
  2. 2.Department of Computer ScienceUniversity of CaliforniaDavisUSA

Personalised recommendations