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The Detection and Isolation of a Novel Antimicrobial Peptide From the Echinoderm Cucumaria Frondosa

  • Karen A. Beauregard
  • Nobel T. Truong
  • Haiyan Zhang
  • Wenyu Lin
  • Gregory Beck
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 484)

Abstract

Microorganisms and their toxins are the cause of many diseases that harm multicellular organisms and the invertebrates are as susceptible to infection by bacteria, fungi and viruses as vertebrates (Marchalonis and Schluter, 1990). The invertebrates have developed highly effective immune systems for detecting and eliminating foreign invaders, consisting of both cellular and humoral/innate components (Bomanet al.1991).

Keywords

Antimicrobial Activity Antimicrobial Peptide Ammonium Sulfate Column Fraction Micrococcus Luteus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Beck G. and Habicht G. S. (1986) Isolation and characterization of a primitive interleukin-1-like protein from an invertebrateAsterias forbesi. Proc Natl Acad Sci USA 83, 7429–7433.CrossRefGoogle Scholar
  2. Beck G. and Habicht G. S. (1991) Purification and biochemical characterization of an invertebrate interleukin 1.Mol Immunol 28, 577–584.PubMedCrossRefGoogle Scholar
  3. Bevins C. L. and Zasloff M. (1990) Peptides from frog skin.Annu Rev Biochem 59, 395–414.PubMedCrossRefGoogle Scholar
  4. Boman H. G., FayeI.Gudmundsson G. H., Lee J. Y. and Lidholm D. A. (1991) Cell-free immunity in Cecropia. A model system for antibacterial proteins.Eur J Biochem 20123–31.PubMedCrossRefGoogle Scholar
  5. Boman H. G. and Hultmark D. (1987) Cell-free immunity in insects.Annu Rev Microbiol 41, 103–126. Canicatti C. (1990) Lysosomal Enzyme Patterns inHolothuriapoliiCoelomocytes.J. Invertebr Pathol 56, 70–74.Google Scholar
  6. Canicatti C. a. D. A. G. (1989) Cellular Aspects ofHolothuria pollImmune Response.J. Invertebr Pathol 53, 152–158.CrossRefGoogle Scholar
  7. Ganz T. and Lehrer R. I. (1998) Antimicrobial peptides of vertebrates.Curr Opin Immunol 10, 41–44.PubMedCrossRefGoogle Scholar
  8. Kaaya G. P. (1993) Inducible Humoral Antibacterial Immunity in Insects. InInsect Immunity(Edited by Pathak J. P. N.), p. 69–89. Oxford & IBH Publishing Co. PVT. LTD., New Delhi, India.Google Scholar
  9. Lee I. H., Cho Y. and Lehrer R. I. (1997) Styelins, broad-spectrum antimicrobial peptides from the solitary tunicate, Styela clava.Comp Biochem Physiol B Biochem Mol Biol 118, 515–521.PubMedCrossRefGoogle Scholar
  10. Lehrer R.I.Michael Rosenman, Sylvia S.S.L. Harwig, Richard Jackson, Patricia Eisenhauer. (1991) Ultrasensitive assays for endogenous antimicrobial polypeptides.Journal of immunological methods 137167–173.PubMedCrossRefGoogle Scholar
  11. Marchalonis J. J. and Schluter S. F. (1990) On the relevance of invertebrate recognition and defence mechanisms to the emergence of the immune response of vertebrates.Scand Jlmmunol 32, 13–20.CrossRefGoogle Scholar
  12. Schagger H. and von Jagow G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.Anal Biochem 166, 368–379.PubMedCrossRefGoogle Scholar
  13. Schnapp D., Kemp G. D. and Smith V. J. (1996) Purification and characterization of a proline-rich antibacterial peptide, with sequence similarity to bactenecin-7, from the haemocytes of the shore crab, Carcinus maenas.Eur JBiochem 240, 532–539.CrossRefGoogle Scholar
  14. Sima P. and Vetvicka V. (1993) Evolution of immune reactions.Crit Rev Immunol 13, 83–114.PubMedGoogle Scholar
  15. Smith L. C. and Davidson E. H. (1994) The echinoderm immune system. Characters shared with vertebrate immune systems and characters arising later in deuterostome phylogeny.Annals Of The New York Academy Of Sciences 712, 213–226.PubMedCrossRefGoogle Scholar
  16. Steinberg D. A. a. L., Robert I. (1997)Designer Assays for Antimicrobial Peptides.Humana Press, Inc., Totowa, New Jersey.Google Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Karen A. Beauregard
    • 1
  • Nobel T. Truong
    • 1
  • Haiyan Zhang
    • 1
  • Wenyu Lin
    • 1
  • Gregory Beck
    • 1
  1. 1.Department of BiologyUniversity of MassachusettsBoston

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