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Structure and Functional Genomics of Lipopolysaccharide Expression inHaemophilus Influenzae

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Book cover The Molecular Immunology of Complex Carbohydrates —2

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 491))

Abstract

The involvement of genes in the lic loci in H. influenzae LPS expression has been known for some time (20). However, it was not until recently that it was shown that the licl locus contains genes required for phase variable expression of phosphocholine substituents (21), while genes in the lic2 locus and lgtC are required for expression of the globoside trisaccharide, α-D-Galp-(1→4)- ß-D-Galp-(1→4)- ß-D-Glcp (i.e., the pK blood group epitope) (19,23). The availability of the complete sequence of the H. influenzae strain Rd genome has facilitated significant progress in understanding the role of these and other genes in the expression and biosynthesis of LPS. We have employed a comparative structural fingerprinting strategy to establish the structural relationships among LPS from H. influenzae mutant strains in which putative biosynthesis genes were inactivated. Using this functional genomics approach, we have gained considerable insight into the genetic basis for intra-strain and strain-to-strain variation in epitope expression.

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Corresponding author: James C. Richards. Fax: (613) 941-1327. E-mail: jim.richards@nrc.ca

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Authors and Affiliations

  1. National Research Council, Institute for Biological Sciences, Ottawa, Ontario, da, K1A OR6

    James C. Richards, Andrew D. Cox & Adele Martin

  2. Clinical Research Centre, University College of South Stockholm, NOVUM S-141 86, Huddinge, Sweden

    Elke K. H. Schweda

  3. Molecular Infectious Diseases Group, University of Oxford, Department of Paediatrics, John Radcliffe Hospital, Headington, Oxford, 0X3 3DU, United Kingdom

    Derek W. Hood & E. Richard Moxon

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  1. James C. Richards
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  2. Andrew D. Cox
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  4. Adele Martin
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Editors and Affiliations

  1. Glyco Research Laboratory Institutes of Basic Medical Sciences and Natural Products, College of Medicine Chang-Gung University, Kwei-san, Tay-yuan, Taiwan

    Albert M. Wu

  2. Tao-yuan, 333, Taiwan

    Albert M. Wu

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© 2001 Springer Science+Business Media New York

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Richards, J.C., Cox, A.D., Schweda, E.K.H., Martin, A., Hood, D.W., Moxon, E.R. (2001). Structure and Functional Genomics of Lipopolysaccharide Expression inHaemophilus Influenzae . In: Wu, A.M. (eds) The Molecular Immunology of Complex Carbohydrates —2. Advances in Experimental Medicine and Biology, vol 491. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1267-7_34

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  • DOI: https://doi.org/10.1007/978-1-4615-1267-7_34

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5469-7

  • Online ISBN: 978-1-4615-1267-7

  • eBook Packages: Springer Book Archive

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