Abstract
A new biotinylated-bipyrrole derivative has been synthesized and its coelectropolymerisation with pyrrole developed for the immobilization of ligands onto electrode surfaces. The biotin-polymeric film was produced on a gold electrode and the FITC-avidin binding to the film surface was demonstrated by fluorescence detection using an epifluorescent microscope.
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BIBLIOGRAPHY
Leipert, D., Heiduschka, P., Mack, J., Egelhaaf, H.J., Oelkrug, D., Jung, G., 1998, Spatially Resolved Immobilization of Peptides by Electrochemical Polymerization after Photolytic Clevage of a Protecting Group. Angew. Chem. Int. Ed. Engl., 37, 17, 2338–2340.
Cosnier, S., 1998, Fabrication of amperometric biosensors by entrapment of enzymes in functionalized polypyrrole films. Can. J. Chem., 76, 1000–1007.
Cosnier, S., Galland, B., Gondran, C., Le Pellec, A., 1998, Electrogeneration of Biotinylated Functionalized Polypyrroles for the Simple Immobilization of Enzymes, Electroanalysis, 10, 12, 808–813
Yang, ST., Witkowski, A., Hutching, R.S., Scott, D.L, Bachas, L.G., 1998, Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide, Electroanalysis, 10, 1, 58–60.
Torres-Rodriguez, L.M., Roget, A., Billon, M., Livache, T., Bidan, G., 1998, Synthesis of a Biotin Functionalized Pyrrole and its Electropolymerization : Toward a Versatile Avidin Biosensor, J. Chem. Soc. Chem. Commun., 1993–1994.
Elming, N., Clauson-Kaas, N., 1952, The Preparation of Pyrroles from Furans, Acta Chem. Scand, 867–874.
Jefford, C.W., De Villedon de Naide, F., Sienkiewicz, K., 1996, The Synthesis of Chiral 1-(lH-Pyrrole) Derivatives, Tetrahedron : Asym., 7, 4, 1069–1076.
8 Bidan, G., Guglielmi, M., 1986, Electrochemical Characteristics of Some N-(Hydroxyalkyl) and N-(Tosylalkyl) Pyrroles ; Electrochemical Behaviour of the Corresponding Polymers, Synth. Metals, 15, 49–58.
Cosnier, S., Lepellec, A., 1999, Poly(pyrrole-biotin) : a New Polymer for Biomolecule Grafting on Electrode Surfaces, Electrochimica Acta, 44, 1833–1836.
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© 2000 Springer Science+Business Media New York
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Perié, K., Strokhin, V., Marks, R.S., Lellouche, JP. (2000). Electropolymerizable Bipyrrole-Biotin: A New Derivative for the Immobilization of Ligands onto Electrode Surfaces. In: Liron, Z., Bromberg, A., Fisher, M. (eds) Novel Approaches in Biosensors and Rapid Diagnostic Assays. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1231-8_16
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DOI: https://doi.org/10.1007/978-1-4615-1231-8_16
Publisher Name: Springer, Boston, MA
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