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Electropolymerizable Bipyrrole-Biotin: A New Derivative for the Immobilization of Ligands onto Electrode Surfaces

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Novel Approaches in Biosensors and Rapid Diagnostic Assays

Abstract

A new biotinylated-bipyrrole derivative has been synthesized and its coelectropolymerisation with pyrrole developed for the immobilization of ligands onto electrode surfaces. The biotin-polymeric film was produced on a gold electrode and the FITC-avidin binding to the film surface was demonstrated by fluorescence detection using an epifluorescent microscope.

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BIBLIOGRAPHY

  1. Leipert, D., Heiduschka, P., Mack, J., Egelhaaf, H.J., Oelkrug, D., Jung, G., 1998, Spatially Resolved Immobilization of Peptides by Electrochemical Polymerization after Photolytic Clevage of a Protecting Group. Angew. Chem. Int. Ed. Engl., 37, 17, 2338–2340.

    CAS  Google Scholar 

  2. Cosnier, S., 1998, Fabrication of amperometric biosensors by entrapment of enzymes in functionalized polypyrrole films. Can. J. Chem., 76, 1000–1007.

    Article  CAS  Google Scholar 

  3. Cosnier, S., Galland, B., Gondran, C., Le Pellec, A., 1998, Electrogeneration of Biotinylated Functionalized Polypyrroles for the Simple Immobilization of Enzymes, Electroanalysis, 10, 12, 808–813

    Article  CAS  Google Scholar 

  4. Yang, ST., Witkowski, A., Hutching, R.S., Scott, D.L, Bachas, L.G., 1998, Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide, Electroanalysis, 10, 1, 58–60.

    Article  CAS  Google Scholar 

  5. Torres-Rodriguez, L.M., Roget, A., Billon, M., Livache, T., Bidan, G., 1998, Synthesis of a Biotin Functionalized Pyrrole and its Electropolymerization : Toward a Versatile Avidin Biosensor, J. Chem. Soc. Chem. Commun., 1993–1994.

    Google Scholar 

  6. Elming, N., Clauson-Kaas, N., 1952, The Preparation of Pyrroles from Furans, Acta Chem. Scand, 867–874.

    Google Scholar 

  7. Jefford, C.W., De Villedon de Naide, F., Sienkiewicz, K., 1996, The Synthesis of Chiral 1-(lH-Pyrrole) Derivatives, Tetrahedron : Asym., 7, 4, 1069–1076.

    Article  CAS  Google Scholar 

  8. 8 Bidan, G., Guglielmi, M., 1986, Electrochemical Characteristics of Some N-(Hydroxyalkyl) and N-(Tosylalkyl) Pyrroles ; Electrochemical Behaviour of the Corresponding Polymers, Synth. Metals, 15, 49–58.

    Article  CAS  Google Scholar 

  9. Cosnier, S., Lepellec, A., 1999, Poly(pyrrole-biotin) : a New Polymer for Biomolecule Grafting on Electrode Surfaces, Electrochimica Acta, 44, 1833–1836.

    Article  CAS  Google Scholar 

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© 2000 Springer Science+Business Media New York

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Perié, K., Strokhin, V., Marks, R.S., Lellouche, JP. (2000). Electropolymerizable Bipyrrole-Biotin: A New Derivative for the Immobilization of Ligands onto Electrode Surfaces. In: Liron, Z., Bromberg, A., Fisher, M. (eds) Novel Approaches in Biosensors and Rapid Diagnostic Assays. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1231-8_16

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  • DOI: https://doi.org/10.1007/978-1-4615-1231-8_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5452-9

  • Online ISBN: 978-1-4615-1231-8

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