Abstract
A highly-sensitive surface fluorescence technique was combined with a powerful method for the oriented reversible immobilization of membrane proteins on oxide surfaces. Affinity and kinetic parameters of the interaction of the receptor and a fluorescent ligand were evaluated. The approach is particularly suited for the study of rapid ligand-receptor interactions where optimized cell volume, flow and injection conditions are required.
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© 2000 Springer Science+Business Media New York
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Schmid, E.L., Hovius, R., Pawlak, M., Ehrat, M., Vogel, H. (2000). Waveguide Fluorosensor for the Detection of Ligand-Receptor Interactions. In: Liron, Z., Bromberg, A., Fisher, M. (eds) Novel Approaches in Biosensors and Rapid Diagnostic Assays. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1231-8_10
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DOI: https://doi.org/10.1007/978-1-4615-1231-8_10
Publisher Name: Springer, Boston, MA
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Online ISBN: 978-1-4615-1231-8
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