Abstract
The glycerol uptake facilitator of E. coli (GlpF) (Boos et al., 1990) is one of the few known diffusion facilitators in the inner membrane of this bacterium. Glycerol diffuses into the cell through GlpF and is phosphorylated by the glycerol kinase (GlpK), which prevents back-diffusion. Besides glycerol transport, the diffusion of polyols and urea derivatives through GlpF have been reported (Maurel et al., 1994), but none of these substrates are transported in a phosphorylated state. In contrast, it remains unclear whether GlpF allows the passage of water.
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References
Boos W Ehmann U Forkl H Klein W Rimmele M and Postma P 1990 Trehalose transport and metabolism in Escherichia coli J Bacteriol 172: 345061
Borgnia MJ Kozono D Calamita G Maloney PC and Agre P 1999 Functional reconstitution and characterization of AqpZ the E coli water channel protein J Mol Biol 291: 11691179
Cheng A van Hoek AN Yeager M Verkman AS and Mitra AK 1997 Three-dimensional organization of a human water channel Nature 387: 627630
Froger A Tallur B Thomas D and Delamarche C 1998 Prediction of functional residues in water channels and related proteins Protein Sci 7: 14581468
Hasler L Walz T Tittmann P Gross H Kistler J and Engel A 1998 Purified lens major intrinsic protein MIP forms highly ordered tetragonal two-dimensional arrays by reconstitution J Mol Biol 279: 855864
Henderson R Baldwin JM Ceska TA Zemlin F Beckmann E and Downing KH 1990 Model for the structure of Bacteriorhodopsin based on high-resolution electron cryo-microscopy J Mol Biol 213: 899- 929
Henderson R Baldwin JM Downing KH Lepault J and Zemlin F 1986 Structure of purple membrane from Halobacterium halobium : recording measurement and evaluation of electron micrographs at 35 Å resolution Ultramicroscopy 19: 147178
Heymann B and Engel A 2000 Structural Clues in the Sequences of the AquaporinsJ Mol Biol 295: 10391053
Jap BK Zulauf M Scheybani T Hefti A Baumeister W Aebi U and Engel A 1992 2D crystallization: from art to science Ultramicroscopy 46: 4584
Jung JS Preston GM Smith BL Guggino WB and Agre P 1994 Molecular structure of the water channel through aquaporin CHIP The hourglass model J Biol Chem 269: 1464814654
Lagree V Froger A Deschamps S Hubert JF Delamarche C Bonnec G Thomas D Gouranton J and Pellerin I 1999 Switch from an aquaporin to a glycerol channel by two amino acids substitution J Biol Chem 274: 68176819
Li H Lee S and Jap BK 1997 Molecular design of aquaporin-1 water channel as revealed by electron crystallography [letter] [see comments] Nature Struct Biol 4 263265
Maurel C Reizer J Schroeder JI Chrispeels MJ and Saier MH Jr 1994 Functional characterization of the Escherichia coli glycerol facilitator GlpF in Xenopus oocytes J Biol Chem 269: 1186911872
Mitsuoka K Murata K Walz T Hirai T Agre P Heymann JB Engel A and Fujiyoshi Y 1999 The Structure of Aquaporin-1 at 45-# Resolution Reveals Short alpha- Helices in the Center of the Monomer J Struct Biol 128: 3443
Mulders SM Preston GM Deen PM Guggino WB van Os CH and Agre P 1995 Water channel properties of major intrinsic protein of lens J Biol Chem 270: 90109016
Park JH and Saier MH Jr 1996 Phylogenetic characterization of the MIP family of transmembrane channel proteins J Membr Biol 153: 171180
Reizer J Reizer A and Saier MH Jr 1993 The MIP family of integral membrane channel proteins: sequence comparisons evolutionary relationships reconstructed pathway of evolution and proposed functional differentiation of the two repeated halves of the proteins Crit Rev Biochem Mol Biol 28: 235257
Ringler P Borgnia MJ Stahlberg H Maloney PC Agre P and Engel A 1999 Structure of the water channel AqpZ from Escherichia coli revealed by electron crystallography J Mol Biol 291: 11811190
Smith BL and Agre P 1991 Erythrocyte Mr 28000 transmembrane protein exists as a multisubunit oligomer similar to channel proteinsJ Biol Chem 266: 64076415
Voegele RT Sweet GD and Boos W 1993 Glycerol kinase of Escherichia coli is activated by interaction with the glycerol facilitator J Bacteriol 175: 10871094
Walz T Hirai T Murata K Heymann JB Mitsuoka K Fujiyoshi Y Smith BL Agre P and Engel A 1997 The three-dimensional structure of Aquaporin-1 Nature 387: 624627
Walz T Smith BL Agre P and Engel A 1994 The three-dimensional structure of human erythrocyte aquaporin CHIP EMBO J 13: 29852993
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Braun, T. et al. (2000). GLPF: A Structural Variant of the Aquaporin Tetramer. In: Hohmann, S., Nielsen, S. (eds) Molecular Biology and Physiology of Water and Solute Transport. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1203-5_2
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DOI: https://doi.org/10.1007/978-1-4615-1203-5_2
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