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Direct 5S rRNA Assay for Microbial Community Characterization

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Thermophiles Biodiversity, Ecology, and Evolution

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Abstract

Research in the geothermal regions of Yellowstone National Park has revealed an abundance of microorganisms that inhabit these extreme environments. The description of these microbial populations has been addressed by a number of approaches. One approach was the cultivation and identification of microorganisms using classical microbiological methods (Brock and Darland, 1971; Brock and Freeze, 1969; Brock et al., 1972). Other approaches use molecular methods that identify microorganisms by sequence analysis of nucleic acids extracted from collected biomass (Stahl et al., 1985), polymerase chain reaction (PCR)-amplified rRNA genes from clones and isolates (Barns et al., 1994; Reysenbach et al., 1994; Saul et al., 1993), and cDNA synthesized from small subunit ribosomal RNA (16S rRNA) extracted from collected biomass (Ward et al., 1990).

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Author information

Authors and Affiliations

  1. Biotechnology, Idaho National Engineering and Environmental Laboratory, Idaho Falls, Idaho, 83415-2203, USA

    D. L. Stoner, D. K. Bulmer & T. E. Ward

  2. Ransom Hill Bioscience, Inc., Ramona, California, 92065, USA

    C. K. Browning & M. T. MacDonell

Authors
  1. D. L. Stoner
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  2. C. K. Browning
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  3. D. K. Bulmer
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  4. T. E. Ward
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  5. M. T. MacDonell
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Editor information

Editors and Affiliations

  1. Portland State University, Portland, Oregon, USA

    Anna-Louise Reysenbach

  2. United States Geological Survey, Reston, Virginia, USA

    Mary Voytek

  3. NASA/Ames Research Center, Moffett Field, California, USA

    Rocco Mancinelli

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Stoner, D.L., Browning, C.K., Bulmer, D.K., Ward, T.E., MacDonell, M.T. (2001). Direct 5S rRNA Assay for Microbial Community Characterization. In: Reysenbach, AL., Voytek, M., Mancinelli, R. (eds) Thermophiles Biodiversity, Ecology, and Evolution. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1197-7_6

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  • DOI: https://doi.org/10.1007/978-1-4615-1197-7_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5436-9

  • Online ISBN: 978-1-4615-1197-7

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