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Presence of Thermophilic Naegleria Isolates in the Yellowstone and Grand Teton National Parks

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Thermophiles Biodiversity, Ecology, and Evolution

Abstract

During a study of the extremely thermophilic bacteria of Yellowstone, we became aware of the published report (Scaglia et al., 1983) of the isolation of a thermophilic amoeba (Naegleria australiensis) from the bathing pools of a commercial Italian hot spring spa and their observation that some of these newly isolated amoebae strains were as virulent for mice as Naegleria fowleri, the etiological agent of primary amoebic meningoencephalitis in humans (John, 1982; Marciano-Cabral, 1988). Because of the possibility that similar types of thermophilic pathogenic amoebae might be present in the Yellowstone and Grand Teton National Park thermal areas and possible exposure to park visitors and employees, we conducted a survey for Naegleria isolates, concentrated on those pools or outflows where it was known that visitors and employees used these pools for unauthorized bathing and soaking (Whittley, 1995).

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References

  • Boyle, J., Kingston, N., and Jolley, W. 1982. Thermophilic amoebae from Yellowstone Hot Springs. Annual Meeting, Am. Soc. Parasit Ab. 31.

    Google Scholar 

  • Brock, T. D., and Freeze, H. 1969. Thermus aquaticus, gen. n. and sp. n., a nonsporulating extreme thermophile. J. Bacteriol. 98:289–297.

    PubMed  CAS  Google Scholar 

  • Cerva, L. 1969. Amoebic meningoencephalitis: axenic culture of Naegleria. Science 163:576.

    PubMed  CAS  Google Scholar 

  • DeJonckheere, J. 1977. Use of an axenic medium for differentiation between pathogenic and non-pathogenic Naegleria fowleri isolates. Appl. Environ. Microbiol. 33:751–757.

    CAS  Google Scholar 

  • DeJonckheere, J., and Van De Voorde, H. 1977. The distribution of Naegleria fowleri in man-made thermal waters. Am. J. Trop. Med. Hyg. 26:10–15.

    CAS  Google Scholar 

  • DeJonckheere, J. F. 1979. Occurrence of Naegleria and Acanthamoeba in aquaria. Appl. Environ. Microbiol. 38:590–593.

    CAS  Google Scholar 

  • DeJonckheere, J. 1981. Naegleria australiensis sp. nov., another pathogenic Naegleria from water. Protistologica 17:423–429.

    Google Scholar 

  • DeJonckheere, J. F., Aerts, M., and Martinez, A. J. 1983. Naegleria australiensis: Experimental meningoencephalitis in mice. Trans. R. Soc. Trop. Med. Hyg. 77:712–716.

    Article  CAS  Google Scholar 

  • DeJonckheere, J. F., Pernin, P., Scaglia, M., and Michel, R. 1984. A comparative study of 14 strains of Naegleria australiensis demonstrates the existence of a highly virulent subspecies: N. australiensis italica n. ssp. J. Protozool 31:324–331.

    CAS  Google Scholar 

  • Jensen, T., and Dubes, G. R. 1962. Cloning, titration, and differentiation of Acanthamoeba sp. by plating. J. Parasitol. 48:280–286.

    Article  PubMed  CAS  Google Scholar 

  • John, D. T. 1982. Primary amebic meningoencephalitis and the biology of Naegleria fowleri. Annu. Rev. Microbiol 63:101–123.

    Article  Google Scholar 

  • John, D. T., Cole, T. B., and Marciano-Cabral, F. 1984. Sucker-like structure on the pathogenic amoeba Naegleria fowleri. Appl Environ. Microbiol. 47:12–14.

    PubMed  CAS  Google Scholar 

  • John, D. T., and Hoppe, K. L. 1990. Susceptibility of wild mammals to infection with Naegleria fowleri. J. Parasitol. 76:865–868.

    Article  PubMed  CAS  Google Scholar 

  • Kilvington, S., and Beeching, J. 1995. Development of a PCR for identification of Naegleria fowleri from the environment. Appl. Environ. Microbiol 61:3764–3767.

    PubMed  CAS  Google Scholar 

  • Kollars, T. M., and Wilhelm, W. E. 1996. The occurrence of antibodies to Naegleria species in wild mammals. J. Parasitol. 82:73–77.

    Article  PubMed  Google Scholar 

  • Kyle, D. E., and Noblet, G. P. 1985. Vertical distribution of potentially pathogenic free-living amoebae in freshwater lakes. J. Protozool. 32:99–105.

    PubMed  CAS  Google Scholar 

  • Loginova, L. G., and Egorova, L. A. 1975. Thermus ruber obligate thermophilic bacteria in thermal springs of Kamchatka. Mikrobiologiia 44:661–665.

    PubMed  CAS  Google Scholar 

  • Marciano-Cabral, F., 1988. Biology of Naegleria spp. Microbiol. Rev. 52:114–133.

    PubMed  CAS  Google Scholar 

  • Martinez, A. J., and Visvesvara, G. S. 1997. Free-living, amphizoic and opportunistic amebas. Brain Pathol. 7:583–598.

    Article  PubMed  CAS  Google Scholar 

  • Mitchell, L. G., Mutchmor, J. A., and Dolphin, W. D. 1988. Zoology. Menlo Park, CA: Benjamin/Cummings.

    Google Scholar 

  • O’Dell, W. D., and Stevens, A. R. 1973. Quantitative growth in Naegleria in axenic culture. Appl. Microbiol. 25:621–627.

    PubMed  Google Scholar 

  • O’Dell, W. D. 1979. Isolation, enumeration and identification of amoebae from a Nebraska lake. J. Protozool. 26:265–269.

    Google Scholar 

  • Page, F. C. 1967. Taxonomic criteria for limax amoebae, with description of 3 new species of Hartmannella and 3 of Vahlkampfia. J. Bacteriol. 98:289–297.

    Google Scholar 

  • Ramaley, R. F., and Hixson, J. 1970. Isolation of a nonpigmented, thermophilic bacterium similar to Thermus aquaticus. J. Bacteriol. 103:527–528.

    PubMed  CAS  Google Scholar 

  • Ramaley, R. F., and Bitzinger, K. 1975. Types and distribution of obligate thermophilic bacteria in man-made and natural thermal gradients. Appl. Microbiol. 30:152–155.

    PubMed  CAS  Google Scholar 

  • Ramaley, R. F., Bitzinger, K., Carroll, R. M., and Wilson, R. B. 1975. Isolation of a new pink thermophilic bacterium (K-2 Isolate). Int. J. Syst. Bacteriol. 25:357–364.

    Article  Google Scholar 

  • Scaglia, M., Storsselli, M., Grazioli, V., Gatti, S., Bernuzzi, A. M. and DeJonckheere, J. F. 1983. Isolation and identification of pathogenic Naegleria australiensis (Amoebida, Vahlkampfidae) from a spa in Northern Italy. Appl. Environ. Microbiol. 46:1282–1285.

    PubMed  CAS  Google Scholar 

  • Scanlan, R L. 1988. Application of the API ZYM system in the identification of Naegleria isolates. M.S. Thesis, University of Nebraska at Omaha.

    Google Scholar 

  • Stevens, A. R., DeJonckheere, J., and Willaert, E. 1980. Naegleria lovaniensis new species: Isolation and identification of six thermophilic strains of a new species found in association with Naegleria fowleri. Int. J. Parasit. 10:51–64.

    Article  CAS  Google Scholar 

  • Thong, Y. H., and Ferrante, A. 1986. Migration pattern of pathogenic and nonpathogenic Nagleria spp. Infect. Immun. 51:177–180.

    PubMed  CAS  Google Scholar 

  • Whittley, L. H. 1995. Death in Yellowstone. Boulder, CO: Roberts Rinehart.

    Google Scholar 

  • Willaert, E. 1971. Isolement et culture in vitro des amibes du genre Naegleria. Ann. Soc. Beige Med. Trop. 51: 701–708.

    CAS  Google Scholar 

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Ramaley, R.F., Scanlan, P.L., O’Dell, W.D. (2001). Presence of Thermophilic Naegleria Isolates in the Yellowstone and Grand Teton National Parks. In: Reysenbach, AL., Voytek, M., Mancinelli, R. (eds) Thermophiles Biodiversity, Ecology, and Evolution. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1197-7_4

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  • DOI: https://doi.org/10.1007/978-1-4615-1197-7_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5436-9

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